Knock-down of aryl hydrocarbon receptor (AhR) affects the lncRNA-mediated response of porcine granulosa cells (AVG-16 cell line) to 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD).

Świgońska, Sylwia; Nynca, Anna; Molcan, Tomasz; Jablonska, Monika; Ciereszko, Renata E.

doi:10.1016/j.anireprosci.2023.107277

Cited by 4 publications

(1 citation statement)

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“…The assembled transcripts were compared with reference annotation using Gffcompare (version 0.12.6; https://doi.org/10.12688/f1000research.2 3297.1, accessed on 5 September 2022). To predict novel lncRNAs, transcripts with class code "x", "o", "i", "u" and "j" were retained [41]. Next, the transcripts with length < 200 nt and exon number < 2 were removed as a potential bias, and coding potential of the remained transcripts was evaluated by means of TransDecoder (version 5.5.0, accessed on 5 September 2022).…”

Section: Identification Of Lncrnamentioning

confidence: 99%

Molecular Action of Tamoxifen in the Ovaries of Rats with Mammary Neoplasia

Nynca,

Swigonska,

Molcan

et al. 2023

IJMS

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Tamoxifen (TAM) is a drug commonly used in patients with breast cancer. The anticancer effect of TAM occurs via its ability to antagonize estrogen-dependent growth of mammary epithelial cells. Previously, we demonstrated that TAM prevented the chemotherapy-induced loss of ovarian follicular reserves in both cancer-free rats and rats with cancer. Such follicular loss is a main cause of infertility in young women treated for cancer. The current study was undertaken to discover the molecules and intracellular pathways involved in the action of TAM in the ovaries of rats with mammary tumors. To meet this goal we used transcriptomic (RNA-Seq) and proteomic (2D-DIGE/MS) approaches. TAM inhibited the expression of genes and lncRNAs involved in ovarian steroidogenesis. Moreover, TAM altered the expression of genes related to primordial follicle activation or arrest. In addition, proteomic screening indicated the importance of basic metabolic processes in the ovarian actions of TAM. Although simple extrapolation of these data to humans is not possible, the results of this study emphasize the need to explore the ability of TAM to affect ovarian function in women undergoing cancer treatment.

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Section: Identification Of Lncrnamentioning

confidence: 99%