Knock-down of a RING finger gene confers cold tolerance

Fang, Huiying; Meng, Qingling; Zhang, Hongsheng; Huang, Ji

doi:10.1080/21655979.2015.1131368

Cited by 15 publications

(6 citation statements)

References 32 publications

(32 reference statements)

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“…OsSRFP1 is known to negatively regulate abiotic stresses particularly cold, salt and oxidative stress via enhancing ROS level in rice (Fang et al . 2015; Fang et al . 2016).…”

Section: Resultsmentioning

confidence: 99%

“…To understand the molecular mechanism of increased ROS level and lower antioxidant activities in the heat sensitive mutants, we tested relative expression level of some stress responsive genes namely SODA , SODB , CATA , CATB and OsSRFP1 (Fang et al . 2015; Fang et al . 2016; Zhao et al .…”

Section: Discussionmentioning

confidence: 99%

“…OsSRFP1 has been reported to be negatively involved in salt and cold tolerance in rice via positively regulating H 2 O 2 level (Fang et al . 2015; Fang et al . 2016).…”

Section: Discussionmentioning

confidence: 99%

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Agronomic, physiological and molecular characterization of rice mutants revealed key role of ROS and catalase in high temperature stress tolerance

Zafar

Hameed

Ashraf

et al. 2019

Preprint

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Plants adapt to harsh environments particularly high temperature stress by regulating their physiological and biochemical processes, which are key tolerance mechanisms. Thus, identification of heat-tolerant rice genotypes and reliable selection indices are crucial for rice improvement programs. Here, we evaluated the response of a rice mutant population for high-temperature stress at the seedling and reproductive stages based on agronomic, physiological and molecular traits. The estimate of variance components revealed significant differences (P<0.001) among genotypes, treatments and their interaction for almost all traits. Principal component analysis showed significant diversity among the genotypes and traits under high-temperature stress. The mutant ‘HTT-121’ was identified as the most heat tolerant mutant with higher grain yield, panicle fertility, cell membrane thermo-stability (CMTS) and antioxidant enzyme levels under heat stress conditions. Various seedling-based morpho-physiological traits (leaf fresh weight, relative water contents, malondialdehyde, CMTS) and biochemical traits (superoxide dismutase, catalase and hydrogen peroxide) explained variations in grain yield that could be used as selection indices for heat tolerance in rice at early growth stages. Notably, heat sensitive mutants showed a significant accumulation of ROS level, reduced activities of catalase and upregulation of OsSRFP1 expression under heat stress, suggesting their key role in regulating heat tolerance in rice. The heat-tolerant mutants identified in this study could be used in breeding programs and the development of mapping populations to unravel the underlying genetic architecture for heat-stress adaptability.Summary text for table of contentsHeat stress probably due to changing climate scenario has become a serious threat for global rice production. On the other side, efforts to develop high yielding cultivars have led to the reduced genetic variability to withstand harsh environmental conditions. This study aimed to identify novel heat tolerant mutants developed through gamma irradiation which will provide a unique genetic resource for breeding programs. Further, we have identified reliable selection indices for screening heat-tolerant rice germplasm at early growth stages.

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“…OsSRFP1 is known to negatively regulate abiotic stresses particularly cold, salt and oxidative stress via enhancing ROS level in rice (Fang et al . 2015; Fang et al . 2016).…”

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Agronomic, physiological and molecular characterization of rice mutants revealed key role of ROS and catalase in high temperature stress tolerance

Zafar

Hameed

Ashraf

et al. 2019

Preprint

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“…The Oryza sativa stress-related RING finger protein 1 (OsSRFP1) is an unusual E3-ubiquitin ligase that shows both transcriptional and post-translational activity in plant response to cold stress ( Fang et al, 2015 , 2016 ). The OsSRFP1 expression level is induced by cold, PEG-simulated drought, salt, H 2 O 2 , and ABA, and its overexpression in rice results in a hypersensitive phenotype and yield penalty although the opposite is observed for RNAi silencing lines under cold stress conditions.…”

Section: E3-ubiquitin Ligases In Temperature Stress Responsesmentioning

confidence: 99%

Modulation of Abiotic Stress Responses in Rice by E3-Ubiquitin Ligases: A Promising Way to Develop Stress-Tolerant Crops

et al. 2021

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Plants are unable to physically escape environmental constraints and have, therefore, evolved a range of molecular and physiological mechanisms to maximize survival in an ever-changing environment. Among these, the post-translational modification of ubiquitination has emerged as an important mechanism to understand and improve the stress response. The ubiquitination of a given protein can change its abundance (through degradation), alter its localization, or even modulate its activity. Hence, ubiquitination increases the plasticity of the plant proteome in response to different environmental cues and can contribute to improve stress tolerance. Although ubiquitination is mediated by different enzymes, in this review, we focus on the importance of E3-ubiquitin ligases, which interact with the target proteins and are, therefore, highly associated with the mechanism specificity. We discuss their involvement in abiotic stress response and place them as putative candidates for ubiquitination-based development of stress-tolerant crops. This review covers recent developments in this field using rice as a reference for crops, highlighting the questions still unanswered.

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“…Transgenic RNAi silenced OsSRFP1 mutant plants obtained enhanced salinity and cold tolerance due to increased antioxidant capacity. It is also proposed as potential candidate target for CRISPRi applications [148,149].…”

Section: Genome Editing Applications For Abiotic Stress Tolerance In mentioning

confidence: 99%

Molecular Abiotic Stress Tolerans Strategies: From Genetic Engineering to Genome Editing Era

Meriç¹,

Ayan²,

Atak³

2021

Abiotic Stress in Plants

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In last decades, plants were increasingly subjected to multiple environmental abiotic stress factors as never before due to their stationary nature. Excess urbanization following the intense industrial applications introduced combinations of abiotic stresses as heat, drought, salinity, heavy metals etc. to plants in various intensities. Technological advancements brought novel biotechnological tools to the abiotic stress tolerance area as an alternative to time and money consuming traditional crop breeding activities as well as they brought vast majority of the problem themselves. Discoveries of single gene (as osmoprotectant, detoxyfying enzyme, transporter protein genes etc.) and multi gene (biomolecule synthesis, heat shock protein, regulatory transcription factor and signal transduction genes etc.) targets through functional genomic approaches identified abiotic stress responsive genes through EST based cDNA micro and macro arrays. In nowadays, genetic engineering and genome editing tools are present to transfer genes among different species and modify these target genes in site specific, even single nuclotide specific manner. This present chapter will evaluate genomic engineering approaches and applications targeting these abiotic stress tolerance responsive mechanisms as well as future prospects of genome editing applications in this field.

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Knock-down of a RING finger gene confers cold tolerance

Cited by 15 publications

References 32 publications

Agronomic, physiological and molecular characterization of rice mutants revealed key role of ROS and catalase in high temperature stress tolerance

Agronomic, physiological and molecular characterization of rice mutants revealed key role of ROS and catalase in high temperature stress tolerance

Modulation of Abiotic Stress Responses in Rice by E3-Ubiquitin Ligases: A Promising Way to Develop Stress-Tolerant Crops

Molecular Abiotic Stress Tolerans Strategies: From Genetic Engineering to Genome Editing Era

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