Knee joint ultrasonography of the ACLT rabbit experimental model of osteoarthritis: relevance and effectiveness in detecting meniscal lesions

Boulocher, C.; Duclos, Marie-Ève; Arnault, Fabien; Roualdes, O.; Fau, Didier; Hartmann, D. J.; Roger, Thierry; Vignon, E.; Viguier, É.

doi:10.1016/j.joca.2007.07.012

Cited by 21 publications

(16 citation statements)

References 36 publications

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“…Moreover, in the 6-24 weeks post-surgical follow-up, the changes such as fibrosis of articular cartilage, loss of full-thickness articular cartilage and osteophyte formation can be observed in this rabbit ACLT model [35]. The imaging changes in ACLT-treated side articular cartilage or meniscus can be detected by ultrasound [36], microtomography (Micro-CT), or magnetic resonance (MR) [37]. In this study, we used ACLT method to establish the animal model of OA.…”

Section: Discussionmentioning

confidence: 83%

Effect of Low-Intensity Pulsed Ultrasound on MMP-13 and MAPKs Signaling Pathway in Rabbit Knee Osteoarthritis

Chen

et al. 2011

Cell Biochem Biophys

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We evaluated the effect of low-intensity pulsed ultrasound (LIPUS) on MMP-13 and MAPKs expression in rabbit knee osteoarthritis (OA). For this purpose, 18 New Zealand white rabbits were randomly and equally divided into O + L, O - L, and SO groups. In O + L group, animals underwent right back leg ACLT operation and LIPUS radiation. In O - L group, animals underwent ACLT but no LIPUS treatment. In SO (control) group, animals underwent sham operation without LIPUS. After 6 weeks, we assessed the pathologic changes in the articular surface of femoral condyle and compared using Mankin scores. Also, expression of type-II collagen, MMP-13, ERK1/2, p38, and JNK was measured by Western blot. Compared with controls, Mankin scores were higher in O + L (P < 0.05)/O - L (P < 0.01) groups. Compared with O + L group, score was higher in O - L group (P < 0.05). Compared with controls, type-II collagen expression was less in O + L/O - L groups, with more significant decrease in O - L group (P < 0.05). Contrarily, expression of MMP-13, p-ERK1/2, and p-p38 was enhanced in O + L/O - L groups as compared with controls, with more significant increase in O - L group (P < 0.01). Compared with O + L group, expression was higher in O - L group (P < 0.05). We, therefore, concluded that LIPUS application promoted cartilage repair in OA through the downregulation of MMP-13, ERK1/2, and p38.

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Section: Discussionmentioning

confidence: 83%

Effect of Low-Intensity Pulsed Ultrasound on MMP-13 and MAPKs Signaling Pathway in Rabbit Knee Osteoarthritis

Chen

et al. 2011

Cell Biochem Biophys

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“…Anterior cruciate ligament transection (ACLT) of the OA rabbit model was performed as described previously (Boulocher et al 2008;Jean et al 2008). Briefly, rabbits were intravenously anesthetized with 3% sodium pentobarbital (1 mL/kg).…”

Section: Surgical Proceduresmentioning

confidence: 99%

Low-Intensity Pulsed Ultrasound Affects Chondrocyte Extracellular Matrix Production via an Integrin-Mediated p38 MAPK Signaling Pathway

Xia

Ren

Lin

et al. 2015

Ultrasound in Medicine & Biology

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“…Rabbits of the control group were not operated on. Rabbits were kept in individual cages and in the same conditions [40,50,51,54]. …”

Section: Methodsmentioning

confidence: 99%

“…To determine the feasibility of a radiographic grading system, three experienced readers reviewed 250 radiographs of rabbit left femorotibial joint from control and CCLT rabbits obtained during previous studies [40,50,51,54]. Subsequently they consensually constructed an original ROA semi-quantitative grading scale that separated joint space narrowing and osteophytosis [35].…”

Section: Methodsmentioning

confidence: 99%

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Radiographic assessment of the femorotibial joint of the CCLT rabbit experimental model of osteoarthritis

et al. 2010

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BackgroundThe purposes of the study were to determine the relevance and validity of in vivo non-invasive radiographic assessment of the CCLT (Cranial Cruciate Ligament Transection) rabbit model of osteoarthritis (OA) and to estimate the pertinence, reliability and reproducibility of a radiographic OA (ROA) grading scale and associated radiographic atlas.MethodsIn vivo non-invasive extended non weight-bearing radiography of the rabbit femorotibial joint was standardized. Two hundred and fifty radiographs from control and CCLT rabbits up to five months after surgery were reviewed by three readers. They subsequently constructed an original semi-quantitative grading scale as well as an illustrative atlas of individual ROA feature for the medial compartment. To measure agreements, five readers independently scored the same radiographic sample using this atlas and three of them performed a second reading. To evaluate the pertinence of the ROA grading scale, ROA results were compared with gross examination in forty operated and ten control rabbits.ResultsRadiographic osteophytes of medial femoral condyles and medial tibial condyles were scored on a four point scale and dichotomously for osteophytes of medial fabella. Medial joint space width was scored as normal, reduced or absent. Each ROA features was well correlated with gross examination (p < 0.001). ICCs of each ROA features demonstrated excellent agreement between readers and within reading. Global ROA score gave the highest ICCs value for between (ICC 0.93; CI 0.90-0.96) and within (ICC ranged from 0.94 to 0.96) observer agreements. Among all individual ROA features, medial joint space width scoring gave the highest overall reliability and reproducibility and was correlated with both meniscal and cartilage macroscopic lesions (rs = 0.68 and rs = 0.58, p < 0.001 respectively). Radiographic osteophytes of the medial femoral condyle gave the lowest agreements while being well correlated with the macroscopic osteophytes (rs = 0.64, p < 0.001).ConclusionNon-invasive in vivo radiography of the rabbit femorotibial joint is feasible, relevant and allows a reproducible grading of experimentally induced OA lesion. The radiographic grading scale and atlas presented could be used as a template for in vivo non invasive grading of ROA in preclinical studies and could allow future comparisons between studies.

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Knee joint ultrasonography of the ACLT rabbit experimental model of osteoarthritis: relevance and effectiveness in detecting meniscal lesions

Cited by 21 publications

References 36 publications

Effect of Low-Intensity Pulsed Ultrasound on MMP-13 and MAPKs Signaling Pathway in Rabbit Knee Osteoarthritis

Effect of Low-Intensity Pulsed Ultrasound on MMP-13 and MAPKs Signaling Pathway in Rabbit Knee Osteoarthritis

Low-Intensity Pulsed Ultrasound Affects Chondrocyte Extracellular Matrix Production via an Integrin-Mediated p38 MAPK Signaling Pathway

Radiographic assessment of the femorotibial joint of the CCLT rabbit experimental model of osteoarthritis

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