Kluyveromyces as promising yeast cell factories for industrial bioproduction: From bio-functional design to applications

Qiu, Yibin; Lei, Peng; Wang, Rui; Sun, Liang; Luo, Zhengshan; Li, Sha; Xu, Hong

doi:10.1016/j.biotechadv.2023.108125

Cited by 8 publications

(7 citation statements)

References 183 publications

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“…K. marxianus has become an attractive non-conventional yeast chassis cell factory due to its excellent industrial properties (Qiu et al 2023 ). Among various products that can be produced by K. marxianus , organic acids have received increasing interest, especially as monomers of bioplastics (Zhang et al 2023 ).…”

Section: Discussionmentioning

confidence: 99%

“…Last but not least, K. marxianus is genetically amenable, which makes it an attractive yeast cell factory. K. marxianus has been used to produce industrial enzymes, fuel ethanol, single-cell proteins, vaccines, the flavoring compound 2-phenylethanol, and lactic acid (Qiu et al 2023 ; Zhou et al 2018 ). For efficient production, it is required that the microbial strains are tolerant to various stress conditions that are relevant to industrial applications, which include high product concentration, low pH, or high temperature (Zhao et al 2016 ; Zhang et al 2023 ).…”

Section: Introductionmentioning

confidence: 99%

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Transcriptome analysis of Kluyveromyces marxianus under succinic acid stress and development of robust strains

Zeng,

Yang,

Wang

et al. 2024

Appl Microbiol Biotechnol

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Kluyveromyces marxianus has become an attractive non-conventional yeast cell factory due to its advantageous properties such as high thermal tolerance and rapid growth. Succinic acid (SA) is an important platform molecule that has been applied in various industries such as food, material, cosmetics, and pharmaceuticals. SA bioproduction may be compromised by its toxicity. Besides, metabolite-responsive promoters are known to be important for dynamic control of gene transcription. Therefore, studies on global gene transcription under various SA concentrations are of great importance. Here, comparative transcriptome changes of K. marxianus exposed to various concentrations of SA were analyzed. Enrichment and analysis of gene clusters revealed repression of the tricarboxylic acid cycle and glyoxylate cycle, also activation of the glycolysis pathway and genes related to ergosterol synthesis. Based on the analyses, potential SA-responsive promoters were investigated, among which the promoter strength of IMTCP2 and KLMA_50231 increased 43.4% and 154.7% in response to 15 g/L SA. In addition, overexpression of the transcription factors Gcr1, Upc2, and Ndt80 significantly increased growth under SA stress. Our results benefit understanding SA toxicity mechanisms and the development of robust yeast for organic acid production. Key points • Global gene transcription of K. marxianus is changed by succinic acid (SA) • Promoter activities of IMTCP2 and KLMA_50123 are regulated by SA • Overexpression of Gcr1, Upc2, and Ndt80 enhanced SA tolerance Graphical Abstract

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of Kluyveromyces marxianus under succinic acid stress and development of robust strains

Zeng,

Yang,

Wang

et al. 2024

Appl Microbiol Biotechnol

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“…The FDA has classified this yeast expression host as GRAS. Compared with other yeast expression systems, K. lactis has the advantages of multicopy gene integration, no background gene expression during Escherichia coli cloning steps, easy genetic manipulation, availability of a fully sequenced genome, and high‐level and scalable expression of recombinant proteins (Qiu et al., 2023). This yeast has been efficiently used for expressing numerous proteins in recent years (Kim & Kim, 2016).…”

Section: Introductionmentioning

confidence: 99%

Bioengineered xylanase from Misiones Argentina rainforest: A bakery enhancement approach

Molina,

Cazzaniga,

Sgroppo

et al. 2024

Journal of Food Science

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In this study, we pursued the heterologous expression of the xylanase gene from Trichoderma atroviride, a native fungus in the province of Misiones, and used it to enhance the textural properties of baked goods through varying enzymatic concentrations. This marks the inaugural exploration into its functionality in the context of bread production. The recombinant xylanase exhibited improved activity, reaching 36,292 U L−1, achieved by supplementing the culture medium with dextrose. Following the optimization of recombinant xylanase concentration, promising results emerged, notably reducing hardness and chewiness parameters of bread significantly. Our findings underscore the potential of this native fungal enzyme for industrial processes, offering a sustainable and efficient means to enhance the quality of baked goods with broad implications for the food industry. No prior research has been documented on the heterologous expression of the xylanase gene derived from T. atroviride, from the Misiones rainforest, expressed in Kluyveromyces lactis.Practical ApplicationThis research, focusing on the isolation and cloning of xylanase enzyme from Trichoderma atroviride, a native fungus in the province of Misiones, offers a valuable tool for improving the texture of bakery products. By optimizing enzyme concentrations, our findings present a practical approach for the food industry, offering a viable solution to improve the overall quality and consumer satisfaction of bakery products.

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“…The nonconventional yeast Kluyveromyces marxianus has attractive characteristics for industrial applications, such as a high growth rate; thermotolerance; a broad spectrum of carbon sources utilization, including xylose, lactose, and inulin; and the ability to produce ethanol, aroma compounds, enzymes, vaccines, bioactive molecules, fructo-oligosaccharides, fatty acids, and single-cell proteins (SCP) [ 1 , 2 , 3 , 4 , 5 ]. SCP, which are the proteins produced by various microorganisms such as bacteria, yeast, algae, and fungi, are a source of easily digestible protein with a balanced amino acid composition.…”

Section: Introductionmentioning

confidence: 99%

Genotypic and Phenotypic Diversity of Kluyveromyces marxianus Isolates Obtained from the Elaboration Process of Two Traditional Mexican Alcoholic Beverages Derived from Agave: Pulque and Henequen (Agave fourcroydes) Mezcal

Lappe-Oliveras,

Avitia,

Sánchez-Robledo

et al. 2023

JoF

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Seven Kluyveromyces marxianus isolates from the elaboration process of pulque and henequen mezcal were characterized. The isolates were identified based on the sequences of the D1/D2 domain of the 26S rRNA gene and the internal transcribed spacer (ITS-5.8S) region. Genetic differences were found between pulque and henequen mezcal isolates and within henequen mezcal isolates, as shown by different branching patterns in the ITS-5.8S phylogenetic tree and (GTG)5 microsatellite profiles, suggesting that the substrate and process selective conditions may give rise to different K. marxianus populations. All the isolates fermented and assimilated inulin and lactose and some henequen isolates could also assimilate xylose and cellobiose. Henequen isolates were more thermotolerant than pulque ones, which, in contrast, presented more tolerance to the cell wall-disturbing agent calcofluor white (CFW), suggesting that they had different cell wall structures. Additionally, depending on their origin, the isolates presented different maximum specific growth rate (µmax) patterns at different temperatures. Concerning tolerance to stress factors relevant for lignocellulosic hydrolysates fermentation, their tolerance limits were lower at 42 than 30 °C, except for glucose and furfural. Pulque isolates were less tolerant to ethanol, NaCl, and Cd. Finally, all the isolates could produce ethanol by simultaneous saccharification and fermentation (SSF) of a corncob hydrolysate under laboratory conditions at 42 °C.

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Kluyveromyces as promising yeast cell factories for industrial bioproduction: From bio-functional design to applications

Cited by 8 publications

References 183 publications

Transcriptome analysis of Kluyveromyces marxianus under succinic acid stress and development of robust strains

Transcriptome analysis of Kluyveromyces marxianus under succinic acid stress and development of robust strains

Bioengineered xylanase from Misiones Argentina rainforest: A bakery enhancement approach

Genotypic and Phenotypic Diversity of Kluyveromyces marxianus Isolates Obtained from the Elaboration Process of Two Traditional Mexican Alcoholic Beverages Derived from Agave: Pulque and Henequen (Agave fourcroydes) Mezcal

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