Klf4 is required for germ-layer differentiation and body axis patterning during<i>Xenopus</i>embryogenesis

Cao, Qing; Zhang, Xuena; Lü, Lei; Yang, Lu; Gao, Jimin; Gao, Yan; Ma, Haihua; Cao, Ying

doi:10.1242/dev.082024

Cited by 14 publications

(32 citation statements)

References 45 publications

(42 reference statements)

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“…The embryos injected with Klf6 mRNA developed a slightly reduced head and significantly decreased posterior structure (Fig. 4E,L), a phenotype resembling Klf4 gain of function (Cao et al, 2012). Overexpression of Klf7 caused dramatic shortening of A-P axis, increased size of cement gland, reduced head structures, heavily pigmented belly protrusion in tail bud embryos, and incomplete closure of neural tube (Fig.…”

Section: Gain Of Function Analyses On Klf Factorsmentioning

confidence: 90%

“…Previous studies have demonstrated that dorsal genes are regulated by different upstream signals, for example the Wnt and nodal pathways. Moreover, Klf factors are also involved in the regulation of Wnt, TGFb, or nodal signaling (Zhang et al, 2006;Boon et al, 2007;Truty et al, 2009;Cao et al, 2012;Sellak et al, 2012;Spittau and Krieglstein, 2012;Dionyssiou et al, 2013;Yi et al, 2014). It might be expected that Klf factors regulate gene expression in the dorsoventral patterning by means of regulation of Wnt or TGFb signaling.…”

Section: Discussionmentioning

confidence: 99%

“…Data from Cao et al (2012). OE, overexpression; KD, knockdown; #, down-regulated expression; ", up-regulated expression; !, unchanged expression; ᭛, expanded expression; NA, not available.…”

Section: Klf8mentioning

confidence: 99%

“…Localized to neural folds, cement gland, trigeminal placode lung primordium, and duodenum and stomach (Cao et al, 2012).…”

Section: Klf4mentioning

confidence: 99%

“…In Xenopus blastula and gastrula embryos, proteins like Ectodermin (Dupont et al, 2005), Foxi1 (Mir et al, 2007), or Sox3 (Zhang and Klymkowsky, 2007) function to maintain the regional identity of germ layers by means of mediating the signals mentioned above. Moreover, our previous studies have manifested that the pluripotency factors Pou5f1 and Klf4 regulate germ layer differentiation through the control of the both maternal and zygotic signals that promote germ layer formation and axis patterning (Cao et al, 2006(Cao et al, , 2007(Cao et al, , 2012.…”

Section: Introductionmentioning

confidence: 99%

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Kruppel‐like factor family genes are expressed during Xenopus embryogenesis and involved in germ layer formation and body axis patterning

Gao

Cao

Lü

et al. 2015

Developmental Dynamics

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Background: Kruppel-like factors (Klfs) are a family of transcription factors consisting of 17 members in mammals, Klf1-Klf17, which are involved in fundamental cellular physiological procedures, such as cell proliferation, differentiation, and apoptosis. However, their functions in embryonic development have been poorly understood. Our previous study has demonstrated that the pluripotency factor Klf4 participates in germ layer formation and axis patterning of Xenopus embryos by means of the regulation of key developmental signals. In the present study, we further investigated comprehensively the expression and functions of the klf family genes, klf2, klf5, klf6, klf7, klf8, klf11, klf15, and klf17, during the embryogenesis of Xenopus laevis. Results: Spatio-temporal expression analyses demonstrate that these genes are transcribed both maternally and zygotically in Xenopus embryos, and during organogenesis and tissue differentiation, they are localized to a variety of placodes and tissues. Gain and loss of function studies manifest that Klf factors play different roles in germ layer formation and body axis patterning. Moreover, each Klf factor exhibits distinct regulatory effects on the expression of genes that are essential for germ layer formation and body axis patterning. Conclusions: These results suggest that Klf factors are involved in the fine-tuning of these genes during early embryogenesis. Developmental Dynamics 244:1328-1346, 2015. V C 2015 Wiley Periodicals, Inc.

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Section: Gain Of Function Analyses On Klf Factorsmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Klf8mentioning

confidence: 99%

“…Localized to neural folds, cement gland, trigeminal placode lung primordium, and duodenum and stomach (Cao et al, 2012).…”

Section: Klf4mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Kruppel‐like factor family genes are expressed during Xenopus embryogenesis and involved in germ layer formation and body axis patterning

Gao

Cao

Lü

et al. 2015

Developmental Dynamics

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An Introduction to Cellular Reprogramming: The Plasticity of Cell Fates and Identities

Smith

Borowski

Laning³

2014

Chemical Biology in Regenerative Medicine

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Germ layer formation during Xenopus embryogenesis: the balance between pluripotency and differentiation

Cao

2015

Sci. China Life Sci.

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The African clawed frog, Xenopus laevis, has long been a model animal for the studies in the fields of animal cloning, developmental biology, biochemistry, cell biology, and physiology. With the aid of Xenopus, major molecular mechanisms that are involved in embryonic development have been understood. Germ layer formation is the first event of embryonic cellular differentiation, which is induced by a few key maternal factors and subsequently by zygotic signals. Meanwhile, another type of signals, the pluripotency factors in ES cells, which maintain the undifferentiated state, are also present during early embryonic cells. In this review, the functions of the pluripotency factors during Xenopus germ layer formation and the regulatory relationship between the signals that promote differentiation and pluripotency factors are discussed. Xenopus, germ layer formation, pluripotency factors, ES cells Citation:Cao Y. Germ layer formation during Xenopus embryogenesis: the balance between pluripotency and differentiation. Sci China Life Sci, 2015, 58: 336-342, doi: 10.1007/s11427-015-4799-2The understanding of the process of embryonic development is not only a field of basic research. The underlying mechanisms also provide critical clues to the causes of many human diseases, for instances, congenital birth defects or cancers. Based on the studies on typical model animals using molecular and biochemical strategies during recent two or three decades, we can now understand roughly the key molecular events that underlie the framework of early embryonic development, e.g., germ layer induction, dorsoventral patterning, etc. Here I will summarize and discuss some research progress in germ layer formation during Xenopus embryogenesis, in particular the functions of pluripotency factors in controlling germ layer development.

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Klf4 is required for germ-layer differentiation and body axis patterning duringXenopusembryogenesis

Cited by 14 publications

References 45 publications

Kruppel‐like factor family genes are expressed during Xenopus embryogenesis and involved in germ layer formation and body axis patterning

Kruppel‐like factor family genes are expressed during Xenopus embryogenesis and involved in germ layer formation and body axis patterning

An Introduction to Cellular Reprogramming: The Plasticity of Cell Fates and Identities

Germ layer formation during Xenopus embryogenesis: the balance between pluripotency and differentiation

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