Klebsiella variicola and Klebsiella quasipneumoniae with capacity to adapt to clinical and plant settings

Martı́nez-Romero, Esperanza; Rodríguez-Medina, Nadia; Beltrán-Rojel, Marilú; Toribio-Jiménez, Jeiry; Garza–Ramos, Ulises

doi:10.21149/8156

Cited by 22 publications

(24 citation statements)

References 39 publications

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“…The fimbriae fimH is a characteristic common among these bacterial species, and urease is a characteristic present in K. quasipneumoniae , K. variicola and K. pneumoniae isolates and absent in K. quasivariicola . These data correlate with a recent in silico analysis of K. quasipneumoniae and K. variicola genomes which identified a mosaic distribution of different virulence-associated genes that would allow it to adapt to clinical settings [43] . In the case of K. quasivariicola , the eventual description of more isolates and genomes may later permit us to detail the potential setting for this bacterial species.…”

Section: Discussionsupporting

confidence: 85%

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Garza–Ramos

Barrios-Camacho

Moreno-Dominguez

et al. 2018

New Microbes and New Infections

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Klebsiella spp. isolates from community-acquired infections were characterized. A total of 39 Klebsiella spp. isolates were obtained from outpatients at four rural hospitals in Mexico (2013–2014). The biochemical tests identified all as being K. pneumoniae. The molecular multiplex-PCR test identified 36 (92.4%) K. pneumoniae isolates and one (2.5%) K. variicola isolate, and phylogenetic analysis of the rpoB gene identified two isolates (5.1%) belonging to K. quasipneumoniae subsp. quasipneumoniae and K. quasivariicola. The last one was confirmed by phylogenetic analysis of six-loci concatenated genes. Mostly the isolates were multidrug resistant; however, a minority were extended-spectrum β-lactamase producing (10.2%). The extended-spectrum β-lactamase CTX-M-15 gene was identified in these isolates. Analysis of biofilm production and the hypermucoviscosity phenotype showed a total of 35 (92.3%) and seven (17.9%) of the isolates were positive for these phenotypes respectively. The K2 (4/39, 10.2%), K5 (2/39, 5.1%) and K54 (1/39, 2.5%) serotypes were identified in seven (17.9%) of the isolates, and only 28.5% (2/7) hypermucoviscous isolates were positive for the K2 and K5 serotypes. In general, the sequence type (ST) analysis and phylogenetic analysis of seven multilocus sequence typing loci were heterogeneous; however, ST29 was the most prevalent ST in the analysed isolates, accounting for 19% (4/21) of the total isolates. Two of the four ST29 isolates had the hypermucoviscosity phenotype. The virulence factors for fimbriae were the most prevalent, followed by siderophores. Community-acquired infections are caused by various species from Klebsiella genus, with different profiles of antibiotic resistance and heterogeneous virulence factors.

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Section: Discussionsupporting

confidence: 85%

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Garza–Ramos

Barrios-Camacho

Moreno-Dominguez

et al. 2018

New Microbes and New Infections

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“…K. variicola is a nitrogen-fixing species closely related to K. pneumoniae (36, 37) which rarely fixes nitrogen. The former has been isolated from plants such as maize, sugarcane and banana, as a plant-growth-promoting root endophyte (inside plant tissues).…”

Section: Discussionmentioning

confidence: 99%

Nitrogen-fixing Klebsiella variicola in feces from herbivorous tortoises

Montes-Grajales

Jiménez

Rogel

et al. 2019

Preprint

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2 25 Abstract 26 Animals feeding on plants (herbivorous) may have nutritional deficiencies and 27 use bacterial nitrogen fixation in guts to compensate unbalanced diets with high carbon 28 and low nitrogen. Using the acetylene reduction assay we searched for nitrogen fixation 29 in the feces from several herbivorous animals in captivity. We detected acetylene 30 reduction in feces from two African spurred tortoises, Centrochelys sulcata and in feces 31 from six Gopherus berlandieri tortoises and isolated nitrogen-fixing klebsiellas from 32 them. Additionally, we performed a gut metagenomic study with Illumina sequencing 33 from a healthy Mexican G. berlandieri tortoise, and the nif genes identified in the feces 34 microbiome matched those from Klebsiella variicola. Fecal bacterial composition from 35 tortoises was similar to that reported from other reptilian guts. 36 3 37 Introduction 38 Nitrogen fixation is a unique biochemical process carried out by nitrogenases 39 encoded by nif genes, which are found in only a few prokaryotes (1). Nitrogen fixation 40 is energetically expensive and nitrogen-fixing bacteria are in many cases minor 41 components of the microbial community that nevertheless provide a valuable ecological 42 service (2). Nitrogen-fixing bacteria are called diazotrophs (3). 43 When associated with nitrogen-fixing bacteria, insects or plants may inhabit in 44 nitrogen poor conditions. Termites contain nitrogen-fixing bacteria in their guts that 45 allow them to grow in wood, similarly wood eating beetles contain nitrogen-fixing 46 bacteria (4). Nitrogen fixation by Klebsiella variicola occurs in the fungal garden of 47 ants (5). A novel betaproteobacterium capable of fixing nitrogen is a recently described 48 symbiont that is transmitted by eggs of carmine cochineals which feed on cacti as the 49 Gopherus berlandieri tortoise does (6). Cacti have high sugar content and are nitrogen 50 poor, conditions that would be favorable for nitrogen fixation in animals that use cacti 51 for food, such as the carmine cochineal or the G. berlandieri tortoise. 52 G. berlandieri, is distributed from North Mexico (states of Coahuila, Nuevo 53 Leon and Tamaulipas) to South-central Texas in United States (7). This species 54 generally maintains restricted mobility by living for prolonged times in the same 55 burrow (8) and its diet is mainly composed of grasses, weeds and cacti. On the other 56 hand, the African spurred tortoise, Centrochelys sulcata, which is also vegetarian, lives 57 in West Africa (9). 58 Metagenomic analyses have contributed to gain a better insight on taxonomic 59 composition and functions of the gut microbiota of various species, including non-60 model organisms, such as sea turtles (10). Gut microbiota may confer adaptability to 61 herbivorous tortoises such as G. berlandieri and C. sulcata naturally exposed to low 4 62 nitrogen-content diets. The aim of this research was to identify nitrogen-fixing species 63 in the gut microbiota of G. berlandieri and C. sulcata. In addition we present one G...

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“…Their use in agriculture should not be encouraged. Among the nitrogen-fixing bacteria isolated from cereals there are human pathogens or potential human pathogens ( Berg et al, 2005 ), such as Burkholderia cepacia and Klebsiella variicola ( Rosenblueth et al, 2004 , 2011 ; Kutter et al, 2006 ; Martínez-Romero et al, 2018 ). B. cepacia complex (BCC) includes seventeen species, some of them responsible for potentially lethal pulmonary infection in immuno-compromised or cystic fibrosis patients, and others are causative agents of infection in animals and plants ( Sawana et al, 2014 ).…”

Section: Diazotrophs May Be Human Pathogensmentioning

confidence: 99%

Nitrogen Fixation in Cereals

et al. 2018

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Cereals such as maize, rice, wheat and sorghum are the most important crops for human nutrition. Like other plants, cereals associate with diverse bacteria (including nitrogen-fixing bacteria called diazotrophs) and fungi. As large amounts of chemical fertilizers are used in cereals, it has always been desirable to promote biological nitrogen fixation in such crops. The quest for nitrogen fixation in cereals started long ago with the isolation of nitrogen-fixing bacteria from different plants. The sources of diazotrophs in cereals may be seeds, soils, and even irrigation water and diazotrophs have been found on roots or as endophytes. Recently, culture-independent molecular approaches have revealed that some rhizobia are found in cereal plants and that bacterial nitrogenase genes are expressed in plants. Since the levels of nitrogen-fixation attained with nitrogen-fixing bacteria in cereals are not high enough to support the plant’s needs and never as good as those obtained with chemical fertilizers or with rhizobium in symbiosis with legumes, it has been the aim of different studies to increase nitrogen-fixation in cereals. In many cases, these efforts have not been successful. However, new diazotroph mutants with enhanced capabilities to excrete ammonium are being successfully used to promote plant growth as commensal bacteria. In addition, there are ambitious projects supported by different funding agencies that are trying to genetically modify maize and other cereals to enhance diazotroph colonization or to fix nitrogen or to form nodules with nitrogen-fixing symbiotic rhizobia.

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Klebsiella variicola and Klebsiella quasipneumoniae with capacity to adapt to clinical and plant settings

Cited by 22 publications

References 39 publications

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Phenotypic and molecular characterization of Klebsiella spp. isolates causing community-acquired infections

Nitrogen-fixing Klebsiella variicola in feces from herbivorous tortoises

Nitrogen Fixation in Cereals

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