Klebsiella pneumoniae ST147 Coproducing NDM-7 Carbapenemase and RmtF 16S rRNA Methyltransferase in Minnesota

Lee, Chang Seop; Vasoo, Shawn; Hu, Fupin; Patel, Robin; Doi, Yohei

doi:10.1128/jcm.01404-14

Cited by 39 publications

(30 citation statements)

References 9 publications

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“…All seven isolates from Afghanistan, including three with rmtF, carried bla NDM in addition to a methylase gene (Table 2). K. pneumoniae producing both RmtF and NDM in isolates with links to India has been previously described (15,16), and our data support the observation that isolates carrying both carbapenemases and 16S methylases are widespread in this region (17)(18)(19). rmtH was found only in isolates from patients treated for injuries sustained during combat operations in Iraq.…”

supporting

confidence: 90%

Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin

et al. 2016

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c 16S rRNA methyltransferases confer resistance to most aminoglycosides, but discriminating their activity from that of aminoglycoside-modifying enzymes (AMEs) is challenging using phenotypic methods. We demonstrate that arbekacin, an aminoglycoside refractory to most AMEs, can rapidly detect 16S methyltransferase activity in Enterobacteriaceae with high specificity using the standard disk susceptibility test. Aminoglycosides play an important role in treating serious infections caused by Gram-positive and -negative organisms, with amikacin, gentamicin, and tobramycin being of particular clinical value (1). In Gram-negative organisms, resistance is primarily mediated by aminoglycoside-modifying enzymes (AMEs), which inactivate the antibiotic through modification of the -OH or -NH 2 groups (2). AMEs are divided into three classes: the ATP (and/or GDP)-dependent aminoglycoside phosphotransferases (APHs), the acetyl-coenzyme A (CoA)-dependent aminoglycoside acetyltransferases (AACs), and the ATP-dependent aminoglycoside nucleotidyltransferases (ANTs) (2). Different classes have different antibiotic substrates, but no single AME confers resistance to all aminoglycosides. In the past decade, transmissible 16S rRNA methyltransferases (16S RMTases) have emerged as a new and worrisome source of aminoglycoside resistance that operates by reducing the affinity of nearly all aminoglycosides for 16S rRNA via ribosomal methylation (3). Since the presence of multiple AMEs can confer resistance to all clinically relevant aminoglycosides, discriminating between AME RMTase activity and 16S RMTase activity using phenotypic methods can be challenging (3).Arbekacin is a semisynthetic aminoglycoside derived from dibekacin (4). It is licensed only in Japan, where it is used to treat septicemia and pneumonia caused by methicillin-resistant Staphylococcus aureus (MRSA). However, it also has broad antibacterial activity against Gram-negative bacteria, including strains that are resistant to amikacin, gentamicin, and tobramycin (5). Recently, the U.S. Food and Drug Administration approved arbekacin for use under a protocol at the Walter Reed National Military Medical Center to treat infections for which no other antibiotic is available. Importantly, arbekacin is refractory to nearly all AMEs with the exception of some members of the AAC(6=)-I family and the bifunctional enzyme AAC(6=)/APH(2==) produced by some strains of S. aureus and enterococci (3,4,(6)(7)(8). However, even though these enzymes acetylate arbekacin, the antibiotic still retains some antimicrobial activity (9). We exploited this characteristic to develop a rapid method to detect 16S RMTase activity in Enterobacteriaceae using arbekacin-impregnated susceptibility disks.125 Escherichia coli, 74 Klebsiella pneumoniae, and 22 other Enterobacteriaceae isolates, including Enterobacter (n ϭ 4), Morganella (n ϭ 2), Proteus (n ϭ 3), Providencia (n ϭ 6), Serratia (n ϭ

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confidence: 90%

Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin

et al. 2016

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“…This is more likely, as screening for these determinants is yet to be incorporated into routine screening panels, and Enterobacteriaceae with such characteristic determinants were not targeted or resistance was viewed as being conferred by aminoglycosidesmodifying enzymes. The emergence of K. pneumoniae co-producing NDM-1 and RmtF methyltransferase was reported recently in the United States [12]. Similarly, K. pneumoniae accounted for the highest RmtF production and RmtF and NDM-1 co-production in this study.…”

supporting

confidence: 79%

Incidence of the novel rmtF and rmtG methyltransferases in carbapenem-resistant Enterobacteriaceae from a hospital in India

Filgona

Banerjee

Anupurba

2015

J Infect Dev Ctries

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“…All of the isolates were positive for bla NDM-7 according to PCR and sequencing results ( Table 2). Enterobacteriaceae with bla NDM-7 are relatively uncommon, having only been previously described in E. coli from France (26), Germany (27), India (28), the United States (29), and Japan (30) and in K. pneumoniae from the United States (29), Spain (31), and Denmark (32). The single biggest risk factor in all patients was a history of recent travel to India with two exceptions; the patient in the French case had previously traveled to Burma, while no connection with the Indian subcontinent was established among the Spanish patients (26,31).…”

Section: Resultsmentioning

confidence: 99%

“…contained the 50-kb plasmid, which was positive for bla NDM and was typed with the IncX3 replicon ( Table 1). The international case reports of E. coli with bla NDM-7 belonged to various sequence types, including ST167 from France (26), ST599 from Germany (27), ST617 from the United States (29), and ST648 from Japan (30). The K. pneumoniae isolates from the United States and Denmark were typed as ST147 (29,32), while the Spanish isolates belonged to ST437 (31).…”

Section: Resultsmentioning

confidence: 99%

“…The international case reports of E. coli with bla NDM-7 belonged to various sequence types, including ST167 from France (26), ST599 from Germany (27), ST617 from the United States (29), and ST648 from Japan (30). The K. pneumoniae isolates from the United States and Denmark were typed as ST147 (29,32), while the Spanish isolates belonged to ST437 (31). The conjugative plasmid with bla NDM-7 from the French isolate was untypeable (26), and bla NDM-7 from the German E. coli isolate was flanked upstream by IS5 and ISAba125 and downstream by ble MBL and was located on a 60-kb IncX3 plasmid (27).…”

Section: Resultsmentioning

confidence: 99%

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Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada

Chen

Peirano

Lynch

et al. 2016

Antimicrob Agents Chemother

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g Enterobacteriaceae with bla NDM-7 are relatively uncommon and had previously been described in Europe, India, the United States, and Japan. This study describes the characteristics of Enterobacteriaceae (Klebsiella pneumoniae Epidemiological and molecular studies are required to better understand the dynamics of transmission, the risk factors, and the reservoirs for bacteria harboring bla NDM-7 . To the best of our knowledge, this is the first report of S. marcescens and E. hormaechei with bla NDM-7 .

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Klebsiella pneumoniae ST147 Coproducing NDM-7 Carbapenemase and RmtF 16S rRNA Methyltransferase in Minnesota

Cited by 39 publications

References 9 publications

Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin

Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin

Incidence of the novel rmtF and rmtG methyltransferases in carbapenem-resistant Enterobacteriaceae from a hospital in India

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada

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Klebsiella pneumoniae ST147 Coproducing NDM-7 Carbapenemase and RmtF 16S rRNA Methyltransferase in Minnesota

Cited by 39 publications

References 9 publications

Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin

Detecting 16S rRNA Methyltransferases in Enterobacteriaceae by Use of Arbekacin

Incidence of the novel rmtF and rmtG methyltransferases in carbapenem-resistant Enterobacteriaceae from a hospital in India

Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla NDM-7 in Enterobacteriaceae from Calgary, Canada

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Molecular Characterization by Using Next-Generation Sequencing of Plasmids Containing bla _NDM-7 in Enterobacteriaceae from Calgary, Canada