Klebsiella pneumoniae outer membrane vesicles induce strong IL-8 expression via NF-κB activation in normal pulmonary bronchial cells

Li, Ping; Peng, Tingxiu; Xiang, Tianxin; Luo, Wanying; Liao, Wenjian; Wei, Dan-Dan; Luo, Shuai; He, Zhiyong; Liu, Peng; Zhang, Wei; Liu, Yang

doi:10.1016/j.intimp.2023.110352

Cited by 1 publication

(1 citation statement)

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“…Significantly, OMVs have exhibited a range of beneficial effects, including antitumor and immunostimulatory properties, opening avenues for their application in tumor immunotherapy. 19,20 Vaccines made from Neisseria meningitidis-derived OMVs were approved many years ago for preventing human meningococcal disease. 21 Several recent studies have shown the great potential of various bacterial-derived OMVs in tumor therapy, drug delivery, and vaccine development.…”

Section: Introductionmentioning

confidence: 99%

Parabacteroides distasonis-Derived Outer Membrane Vesicles Enhance Antitumor Immunity Against Colon Tumors by Modulating CXCL10 and CD8+ T Cells

Liang,

Li,

Yang

et al. 2024

DDDT

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Given the potent immunostimulatory effects of bacterial outer membrane vesicles (OMVs) and the significant anti-colon tumor properties of Parabacteroides distasonis (Pd), this study aimed to elucidate the role and potential mechanisms of Pd-derived OMVs (Pd-OMVs) against colon cancer. Methods: This study isolated and purified Pd-OMVs from Pd cultures and assessed their characteristics. The effects of Pd-OMVs on CT26 cell uptake, proliferation, and invasion were investigated in vitro. In vivo, a CT26 colon tumor model was used to investigate the anti-colon tumor effects and underlying mechanisms of Pd-OMVs. Finally, we evaluated the biosafety of Pd-OMVs. Results: Purified Pd-OMVs had a uniform cup-shaped structure with an average size of 165.5 nm and a zeta potential of approximately −9.56 mV, and their proteins were associated with pathways related to immunity and apoptosis. In vitro experiments demonstrated that CT26 cells internalized the Pd-OMVs, resulting in a significant decrease in their proliferation and invasion abilities. Further in vivo studies confirmed the accumulation of Pd-OMVs in tumor tissues, which significantly inhibited the growth of colon tumors. Mechanistically, Pd-OMVs increased the expression of CXCL10, promoting infiltration of CD8 + T cells into tumor tissues and expression of pro-inflammatory factors TNF-α, IL-1β, and IL-6. Notably, Pd-OMVs demonstrated a high level of biosafety. Conclusion: This paper elucidates that Pd-OMVs can exert significant anti-colon tumor effects by upregulating the expression of the chemokine CXCL10, thereby increasing the infiltration of CD8 + T cells into tumors and enhancing antitumor immune responses. This suggests that Pd-OMVs may be developed as a novel nanoscale potent immunostimulant with great potential for application in tumor immunotherapy. As well as developed as a novel nano-delivery carrier for combination with other antitumor drugs.

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Section: Introductionmentioning

confidence: 99%