Klebsiella jilinsis 2N3 promotes maize growth and induces resistance to northern corn leaf blight

Zhang, Meng; Zhang, Cheng; Zhang, Sisheng; Yu, Huilin; Pan, Hongyu; Zhang, Hao

doi:10.1016/j.biocontrol.2021.104554

Cited by 16 publications

(9 citation statements)

References 55 publications

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“…Defensive enzymes in plants, such as superoxide dismutase (SOD), peroxidase (POD), and catalase (CAT), have been confirmed to be involved in defense responses against plant pathogens [ 34 ]. Here, the activities of these enzymes in potato plant leaves were measured after being pretreated with FLBS, BCS, or FL.…”

Section: Resultsmentioning

confidence: 99%

Bacillus velezensis Strain HN-Q-8 Induced Resistance to Alternaria solani and Stimulated Growth of Potato Plant

Bai

Zhang

et al. 2023

Biology

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Bacillus velezensis HN-Q-8, isolated in our previous study, has an antagonistic effect on Alternaria solani. After being pretreated with a fermentation liquid with HN-Q-8 bacterial cell suspensions, the potato leaves inoculated with A. solani displayed smaller lesion areas and less yellowing than the controls. Interestingly, the activity levels of superoxide dismutase, peroxidase, and catalase in potato seedlings were enhanced by the addition of the fermentation liquid with bacterial cells. Additionally, the overexpression of key genes related to induced resistance in the Jasmonate/Ethylene pathway was activated by the addition of the fermentation liquid, suggesting that the HN-Q-8 strain induced resistance to potato early blight. In addition, our laboratory and field experiments showed that the HN-Q-8 strain can promote potato seedling growth and significantly increase tuber yield. The root activity and chlorophyll content of potato seedlings were significantly increased along with the levels of indole acetic acid, gibberellic acid 3, and abscisic acid upon addition of the HN-Q-8 strain. The fermentation liquid with bacterial cells was more efficient in inducing disease resistance and promoting growth than bacterial cell suspensions alone or the fermentation liquid without bacterial cells. Thus, the B. velezensis HN-Q-8 strain is an effective bacterial biocontrol agent, augmenting the options available for potato cultivation.

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Section: Resultsmentioning

confidence: 99%

Bacillus velezensis Strain HN-Q-8 Induced Resistance to Alternaria solani and Stimulated Growth of Potato Plant

Bai

Zhang

et al. 2023

Biology

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“…The qRT-PCR program used was as follows: 95 • C for 2 min, followed by 32 cycles of 95 • C for 30 s, 60 • C for 30 s, 72 • C for 30 s and 78 • C for 11 s. The melting curve cycle program was as follows: 95 • C for 10 s, 60 • C for 1 min, and 97 • C for 1 s. The relative levels of expression of the genes were determined using the 2 −∆∆CT method, and the 16S rDNA gene was used as a reference gene. Data are reported on a logarithmic scale as a relative gene expression ratio (RQ) after calibration on values obtained at B. mojavensis D50 that was cultured in basic medium [31]. The experiments were replicated three times and repeated three times.…”

Section: B Mojavensis D50 Biofilm-related Analysis Of Differentially ...mentioning

confidence: 99%

“…The experiments were replicated three times and repeated three times. The biochemical growth parameters of tomato plant were measured as described by Zhang et al [31].…”

Section: Effect Of Metal Ions and Culture Conditions On The Biocontro...mentioning

confidence: 99%

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Zheng

Sun

et al. 2023

JoF

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Bacillus mojavensis D50, a biocontrol strain, is used to prevent and treat the fungal plant pathogen Botrytis cinerea. Bacillus mojavensis D50’s biofilms can affect its colonization; thus, the effects of different metal ions and culture conditions on biofilm formation were determined in this study. The results of medium optimization showed that Ca2+ had the best ability to promote biofilm formation. The optimal medium composition for the formation of biofilms contained tryptone (10 g/L), CaCl2 (5.14 g/L), and yeast extract (5.0 g/L), and the optimal fermentation conditions included pH 7, a temperature of 31.4 °C, and a culture time of 51.8 h. We found that the antifungal activity and abilities to form biofilms and colonize roots were improved after optimization. In addition, the levels of expression of the genes luxS, SinR, FlhA, and tasA were up-regulated by 37.56-, 2.87-, 12.46-, and 6.22-fold, respectively. The soil enzymatic activities which related biocontrol-related enzymes were the highest when the soil was treated by strain D50 after optimization. In vivo biocontrol assays indicated that the biocontrol effect of strain D50 after optimization was improved.

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“…Klebsiella pneumoniae NG14 has nitrogen-fixing activity and colonizes the root surface and vascular tissue of rice ( Liu et al, 2011 ). Klebsiella jilinsis 2N3 induces resistance to corn leaf blight caused by Setosphaeria turcica , a hemibiotrophic ascomycete fungal pathogen, and promotes maize growth ( Zhang et al, 2021 ). In addition, Bacillus cereus Sneb 560, B. subtilis Sneb 815, Pseudomonas putida Sneb 821, P. fluorescens Sneb 825, and Serratia proteamaculans Sneb 821 isolated from the rhizosphere soil show strong larvicidal and nematicidal activity in vitro and promote higher root and shoot growth compared to the control when tomato seeds were treated in pot experiments ( Zhao et al, 2018 ).…”

Section: Introductionmentioning

confidence: 99%

Biological Control Efficacy and Action Mechanism of Klebsiella pneumoniae JCK-2201 Producing Meso-2,3-Butanediol Against Tomato Bacterial Wilt

et al. 2022

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Bacterial wilt caused by Ralstonia solanacearum is a fatal disease that affects the production of tomatoes and many other crops worldwide. As an effective strategy to manage bacterial wilt, biological control agents using plant growth-promoting rhizobacteria (PGPR) are being developed. In this study, we screened 2,3-butanediol (BDO)-producing PGPR to control tomato bacterial wilt and investigated the action mechanism of the disease control agent. Of the 943 strains isolated from soil, Klebsiella pneumoniae strain JCK-2201 produced the highest concentration of 2,3-BDO. The culture broth of K. pneumoniae JCK-2201 did not show any direct activity on R. solanacearum in vitro, but a 100-fold dilution effectively controlled tomato bacterial wilt with a control value of 77% in vivo. Fermentation utilizing K. pneumoniae JCK-2201 was optimized to produce 48 g/L of meso-2,3-BDO, which is 50% of the sucrose conversion efficiency. In addition, the control efficacy and mechanism of meso-2,3-BDO produced by JCK-2201 in tomato bacterial wilt were determined by comparative analysis with Bacillus licheniformis DSM13 producing meso-2,3-BDO and B. licheniformis DSM13 ΔalsS that did not produce 2,3-BDO, as the step of converting pyruvate to α-acetolactate was omitted. Tomato seedlings treated with the K. pneumoniae JCK-2201 (500-fold dilution) and B. licheniformis DSM13 (100-fold dilution) culture broth produced meso-2,3-BDO that significantly reduced R. solanacearum-induced disease severity with control values of 55% and 63%, respectively. The formulated meso-2,3-BDO 9% soluble concentrate (SL; 1,000-fold dilution) showed 87% control against tomato bacterial wilt in the field condition. Klebsiella pneumoniae JCK-2201 and B. licheniformis DSM13 treatment induced the expression of plant defense marker genes, such as LePR1, LePR2, LePR5, LePR3, and PI-II, in the salicylic acid and jasmonic acid signaling pathways at 4 days after inoculation. These results show that 2,3-BDO-producing bacteria and 2,3-BDO are potential biological control agents that act through induction of resistance for controlling tomato bacterial wilt.

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Klebsiella jilinsis 2N3 promotes maize growth and induces resistance to northern corn leaf blight

Cited by 16 publications

References 55 publications

Bacillus velezensis Strain HN-Q-8 Induced Resistance to Alternaria solani and Stimulated Growth of Potato Plant

Bacillus velezensis Strain HN-Q-8 Induced Resistance to Alternaria solani and Stimulated Growth of Potato Plant

Adding Metal Ions to the Bacillus mojavensis D50 Promotes Biofilm Formation and Improves Ability of Biocontrol

Biological Control Efficacy and Action Mechanism of Klebsiella pneumoniae JCK-2201 Producing Meso-2,3-Butanediol Against Tomato Bacterial Wilt

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