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Yahiro, Kazutoyo; Takahama, Tetsusi; Jai, S D; Park, Yong Soo; Okabe, Mitsuyasu

doi:10.1023/a:1018374428391

Cited by 32 publications

(5 citation statements)

References 2 publications

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“…In addition, the advantage of the yeast-like, single cell growth of U. maydis will facilitate a scale-up in larger stirred tank bioreactors. For A. terreus , air lift bioreactors were shown to yield better fermentation results than stirred tank reactors [39], most likely due the sensibility of A. terreus mycelia to hydromechanical stress [40]. In contrast, interaction between cellulases and their substrates requires complete suspension of the cellulose and, therefore, high power input [41].…”

Section: Resultsmentioning

confidence: 99%

Biomass pretreatment affects Ustilago maydis in producing itaconic acid

et al. 2012

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BackgroundIn the last years, the biotechnological production of platform chemicals for fuel components has become a major focus of interest. Although ligno-cellulosic material is considered as suitable feedstock, the almost inevitable pretreatment of this recalcitrant material may interfere with the subsequent fermentation steps. In this study, the fungus Ustilago maydis was used to produce itaconic acid as platform chemical for the synthesis of potential biofuels such as 3-methyltetrahydrofuran. No studies, however, have investigated how pretreatment of ligno-cellulosic biomass precisely influences the subsequent fermentation by U. maydis. Thus, this current study aims to first characterize U. maydis in shake flasks and then to evaluate the influence of three exemplary pretreatment methods on the cultivation and itaconic acid production of this fungus. Cellulose enzymatically hydrolysed in seawater and salt-assisted organic-acid catalysed cellulose were investigated as substrates. Lastly, hydrolysed hemicellulose from fractionated beech wood was applied as substrate.ResultsU. maydis was characterized on shake flask level regarding its itaconic acid production on glucose. Nitrogen limitation was shown to be a crucial condition for the production of itaconic acid. For itaconic acid concentrations above 25 g/L, a significant product inhibition was observed. Performing experiments that simulated influences of possible pretreatment methods, U. maydis was only slightly affected by high osmolarities up to 3.5 osmol/L as well as of 0.1 M oxalic acid. The production of itaconic acid was achieved on pretreated cellulose in seawater and on the hydrolysed hemicellulosic fraction of pretreated beech wood.ConclusionThe fungus U. maydis is a promising producer of itaconic acid, since it grows as single cells (yeast-like) in submerged cultivations and it is extremely robust in high osmotic media and real seawater. Moreover, U. maydis can grow on the hemicellulosic fraction of pretreated beech wood. Thereby, this fungus combines important advantages of yeasts and filamentous fungi. Nevertheless, the biomass pretreatment does indeed affect the subsequent itaconic acid production. Although U. maydis is insusceptible to most possible impurities from pretreatment, high amounts of salts or residues of organic acids can slow microbial growth and decrease the production. Consequently, the pretreatment step needs to fit the prerequisites defined by the actual microorganisms applied for fermentation.

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Section: Resultsmentioning

confidence: 99%

Biomass pretreatment affects Ustilago maydis in producing itaconic acid

et al. 2012

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“…To overcome this issue, air lift reactors represent a suitable alternative for IA production, as it provides good mixing and oxygen transfer rates due to the liquid recirculation [145,153]. Using glucose as carbon source, Yahiro et al produced 63.7 g L -1 of IA in an air lift reactor after seven days of cultivation [154]. Other fermentation strategies are reported in literature, e.g., a fed-batch cultivation as reported by Krull et al [155].…”

Section: Itaconic Acidmentioning

confidence: 99%

“…Using glucose as carbon source, Yahiro et al. produced 63.7 g L −1 of IA in an air lift reactor after seven days of cultivation 154. Other fermentation strategies are reported in literature, e.g., a fed‐batch cultivation as reported by Krull et al.…”

Section: Organic Acids Production and Their Applicationsmentioning

confidence: 99%

Fungal‐Based Biorefinery: From Renewable Resources to Organic Acids

Varriale

Ulber

2023

ChemBioEng Reviews

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Biorefineries are facilities in which lignocellulosic biomasses are converted in a wide range of bioproducts facilitating the transition from the use of petrochemical resources to renewable ones. Organic acids are considered very attractive for their utilization in different industrial areas as building blocks or as final bioproducts leading to a considerable market growth. They are metabolites which are naturally produced by microbials. The production of these molecules by filamentous fungi are attracting more attention due to their ability to hydrolyze lignocellulosic biomasses and to contextually produce different organic acids. Contrarily to a lot of other microorganisms, fungi have the ability to ferment pentoses, broadening the substrate utilization. The integrated use of lignocellulosic biomasses as material input and fungi as biocatalyst can contribute to make biorefineries more successful. This review gives an overview about the lignocellulosic biomass structure and hydrolysis, fungal morphology, and how they are connected. Further, it describes some relevant organic acids with regard to their processes, biocatalysts, industrial applications, and market considerations.

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“…Nemestóthy et al [50] have shown the importance of better oxygen mass transfer: increasing oxygen supply rate and stirring A. terreus in bioreactors under submerged conditions increased the acid production from 1.27 g/L day to 4-26 g/L day. Following this line of studies, air-lift bioreactors have shown advantages in comparison with stirred bioreactors as the itaconic acid production increased from 0.48g/L h to 0.64 g/L h at lower power input [51]. An effective method for increasing itaconic acid production by in-situ reactive acid extraction was recently proposed [52].…”

Section: Aspergillus Terreus As Itaconic Acid Producermentioning

confidence: 99%

Aspegillus terreus: From Soil to Industry and Back

2020

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Aspergillus terreus is an important saprophytic filamentous fungus that can be found in soils. Like many other soil microorganisms, A. terreus demonstrates multiple functions and offers various important metabolites, which can be used in different fields of human activity. The first application of A. terreus on an industrial level is the production of itaconic acid, which is now considered as one of the most important bioproducts in the Green Chemistry field. The general schemes for itaconic acid production have been studied, but in this mini-review some lines of future research are presented based on analysis of the published results. A. terreus is also intensively studied for its biocontrol activity and plant growth-promoting effect. However, this microorganism is also known to infect important crops such as, amongst others, rice, wheat, potato, sugar cane, maize, and soybean. It was suggested, however, that the balance between positive vs. negative effects is dependent on the soil-plant-inoculant dose system. A. terreus has frequently been described as an important human pathogen. Therefore, its safety manipulation in biotechnological processes for the production of itaconic acid and some drugs and its use in soil-plant systems should be carefully assessed. Some suggestions in this direction are discussed, particularly concerning the uses in crop production.

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Cited by 32 publications

References 2 publications

Biomass pretreatment affects Ustilago maydis in producing itaconic acid

Biomass pretreatment affects Ustilago maydis in producing itaconic acid

Fungal‐Based Biorefinery: From Renewable Resources to Organic Acids

Aspegillus terreus: From Soil to Industry and Back

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