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Gariou-Papalexiou, Angeliki; Gourzi, Polyxeni; Delprat, Alejandra; Kritikou, Dimitra; Rapti, Katerina; Chrysanthakopoulou, B; Mintzas, Anastassios C.; Zacharopoulou, A.

doi:10.1023/a:1020959608886

Cited by 28 publications

(13 citation statements)

References 71 publications

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“…Later comparative studies using biochemical and morphological markers showed a remarkable conservation of linked gene clusters among a wider phylogenetic range of higher Diptera 124–126 . In situ hybridization of well characterized genes on polytene chromosomes further supported the extensive conservation of linkage groups in Tephitidae 70, 117, 127–131 . On the other hand, the differences identified in the structure of the 3L and 5L polytene arms of C .…”

Section: Resultsmentioning

confidence: 78%

“…fasciventris to the reference map of C . capitata 67, 117 revealed significant similarities. Characteristic structures such as the distal ends (telomeres) and the centromeric region of each chromosome seem identical between the two species.…”

Section: Resultsmentioning

confidence: 90%

“…melanogaster white gene Bactrocera tryoni genomic 67-5L 65-5L 120, 147 sxl The orthologue of the D . melanogaster sex lethal gene Bactrocera oleae cDNA 79-5R 79-5R 117, 148 st The orthologue of the D . melanogaster scarlet gene Bactrocera tryoni genomic 83-6L 83-6L 117, 149

Figure 6 In situ hybridization on the salivary gland polytene chromosomes of Ceratitis fasciventris .…”

Section: Resultsmentioning

confidence: 99%

“…melanogaster sex lethal gene Bactrocera oleae cDNA 79-5R 79-5R 117, 148 st The orthologue of the D . melanogaster scarlet gene Bactrocera tryoni genomic 83-6L 83-6L 117, 149

Figure 6 In situ hybridization on the salivary gland polytene chromosomes of Ceratitis fasciventris . ( a ) hybridization site of the hsp70 probe; ( b ) hybridization site of the w probe; ( c ) hybridization site of the sxl probe and ( d ) hybridization site of the st probe.…”

Section: Resultsmentioning

confidence: 99%

“…capitata at putative corresponding polytene chromosome regions 70, 117, 120, 121 . The localization of the sxl and s t genes supports the conservation of the 5R and 6L polytene chromosome arms between the two species, as it was deduced by banding pattern similarities (Supplementary Figs S6 and S7).…”

Section: Resultsmentioning

confidence: 99%

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The chromosomes and the mitogenome of Ceratitis fasciventris (Diptera: Tephritidae): two genetic approaches towards the Ceratitis FAR species complex resolution

Drosopoulou

Pantelidou

Gariou-Papalexiou

et al. 2017

Sci Rep

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Ceratitis fasciventris is a serious agricultural pest of the Tephritidae family that belongs to the African Ceratitis FAR species complex. Species limits within the FAR complex are obscure and multidisciplinary approaches have attempted to resolve phylogenetic relationships among its members. These studies support the existence of at least three additional species in the complex, C. anonnae, C. rosa and C. quilicii, while they indicate the presence of two structured populations (F1 and F2) within the C. fasciventris species. In the present study we present the mitotic karyotype, polytene chromosome maps, in situ hybridization data and the complete mitochondrial genome sequence of an F2 population of C. fasciventris. This is the first polytene chromosome map and complete mitogenome of a member of the FAR complex and only the second reported for the Ceratitis genus. Both polytene chromosomes and mitochondrial sequence could provide valuable information and be used as reference for comparative analysis among the members of the complex towards the clarification of their phylogenetic relationships.

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Section: Resultsmentioning

confidence: 78%

Section: Resultsmentioning

confidence: 90%

Figure 6 In situ hybridization on the salivary gland polytene chromosomes of Ceratitis fasciventris .…”

Section: Resultsmentioning

confidence: 99%

“…melanogaster sex lethal gene Bactrocera oleae cDNA 79-5R 79-5R 117, 148 st The orthologue of the D . melanogaster scarlet gene Bactrocera tryoni genomic 83-6L 83-6L 117, 149

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

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The chromosomes and the mitogenome of Ceratitis fasciventris (Diptera: Tephritidae): two genetic approaches towards the Ceratitis FAR species complex resolution

Drosopoulou

Pantelidou

Gariou-Papalexiou

et al. 2017

Sci Rep

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cDNA cloning, characterization, and developmental expression of the 20S proteasome α5 subunit in the Mediterranean fruit fly Ceratitis capitata

Verras

Gourzi

Kalosaka

et al. 2007

Arch Insect Biochem Physiol

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In the present study, we report the cDNA cloning, characterization, and developmental expression of the 20S proteasome alpha5 subunit from the Mediterranean fruit fly Ceratitis capitata (medfly). Using an RT-PCR fragment that corresponds to the amino-terminal region of the Drosophila melanogaster 20S proteasome alpha5 subunit, we isolated a 987-bp cDNA that encodes the complete coding region of the medfly ortholog, which was named CcPSMA5. CcPSMA5 consists of 241 amino acids and has a predicted molecular weight of 26.4 kDa and pI 4.75. Comparison of the CcPSMA5 amino acid sequence with the sequences of all known 20S proteasome alpha5 subunits from different organisms indicated that the medfly 20S proteasome alpha5 subunit has the strongest homology to that of Drosophila. In situ hybridization showed that the CcPSMA5 gene is mapped in the region 44B of chromosome 4. Northern blot hybridization analysis showed that the CcPSMA5 mRNA has a size of approximately 1.2 kb. High levels of the CcPSMA5 mRNA were detected in freshly laid eggs, indicating that they were maternally deposited. The mRNA expression pattern during medfly development suggests that the CcPSMA5 gene is upregulated before mid-embryogenesis and at the onset of metamorphosis.

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Isolation and characterization of microsatellite markers from the Mediterranean fruit fly, Ceratitis capitata: cross-species amplification in other Tephritidae species reveals a varying degree of transferability

et al. 2009

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The Mediterranean fruit fly, Ceratitis capitata, is a pest of major economic importance and has become a model for the development of SIT control programs for insect pests. Significant information has been accumulated on classical and population genetics of this species during the past 2 decades. However, the availability of molecular markers is limited. Here, we present the isolation and characterization of 159 microsatellite clones and the development of 108 polymorphic microsatellite markers for this insect pest. Mapping by in situ hybridization to polytene chromosomes of 21 microsatellite clones enriched the cytogenetic map that was previously constructed by our group. The enriched map provides a large number of STSs for future genome mapping projects. Cross-species amplification of these microsatellite loci in 12 Tephritidae species and sequence analysis of several amplification products indicated a varying degree of transferability and their possible usefulness as molecular and genetic markers in these species where genetic and molecular tools are limited.

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Cited by 28 publications

References 71 publications

The chromosomes and the mitogenome of Ceratitis fasciventris (Diptera: Tephritidae): two genetic approaches towards the Ceratitis FAR species complex resolution

The chromosomes and the mitogenome of Ceratitis fasciventris (Diptera: Tephritidae): two genetic approaches towards the Ceratitis FAR species complex resolution

cDNA cloning, characterization, and developmental expression of the 20S proteasome α5 subunit in the Mediterranean fruit fly Ceratitis capitata

Isolation and characterization of microsatellite markers from the Mediterranean fruit fly, Ceratitis capitata: cross-species amplification in other Tephritidae species reveals a varying degree of transferability

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