Untitled

Ho, Jason Yen-Ping; Chen, Ming‐Jer; Yi, Yu-Chiao; Guu, Hwa-Fen; Ho, Esther Shih-Chu

doi:10.1023/a:1025476910771

Cited by 42 publications

(20 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…It has been reported that a preincubation period between oocyte retrieval and injection in ICSI cycles improved the percentage of mature oocytes [5, 6], the fertilization rate [1, 6, 7], and the embryo quality [1, 2]. A long oocyte preincubation (9–11 hours) prior to ICSI is thought to have bad effects on embryo quality [2], probably due to oocyte ageing.…”

Section: Introductionmentioning

confidence: 99%

“…Although it has been shown that incubation of oocytes for 2–6 h prior to IVF ( In Vitro Fertilization) improves fertilization and pregnancy rates [ 9 – 12 ], there are some conflicting results regarding the timing of ICSI. It has been reported that a preincubation period between oocyte retrieval and injection in ICSI cycles improved the percentage of mature oocytes [ 5 , 6 ], the fertilization rate [ 1 , 6 , 7 ], and the embryo quality [ 1 , 2 ]. A long oocyte preincubation (9–11 hours) prior to ICSI is thought to have bad effects on embryo quality [ 2 ], probably due to oocyte ageing.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection

Patrat

Kaffel

Delaroche

et al. 2012

Obstetrics and Gynecology International

View full text Add to dashboard Cite

Objectives. To analyze the impact of oocyte denudation and microinjection timings on intracytoplasmic sperm injection (ICSI) outcomes. Study Design. We included ICSI cycles with the following parameters: rank 1 or 2, female age <36 years, male factor infertility, long protocol using GnRH agonist and rFSH for ovarian stimulation, and use of freshly ejaculated sperm (n = 110). Several ICSI parameters were analyzed according to the time between oocyte retrieval and denudation (T 1) and the time between denudation and ICSI (T 2) using a statistical logistic regression analysis. Results. Neither T 1 nor T 2 had a significant influence on the Metaphase II (MII) rate but the fertilisation rate (FR) showed a significant improvement when T 1 was longer (optimal results at T 1 = 3 hours) while FR significantly decreased with the increase of T 2. Optimal implantation (IR) and pregnancy (PR) rates were obtained when T 1 was around 2 hours. Conclusion. Incubation of oocytes around 2 hours between retrieval and denudation may not increase MII rate but appears to lead to the optimal combination of FR and IR.

show abstract

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection

Patrat

Kaffel

Delaroche

et al. 2012

Obstetrics and Gynecology International

View full text Add to dashboard Cite

show abstract

“…Although being of paramount clinical importance, the timing of fertilization remains a subject of controversy. Clinical data support the notion that a pre-incubation interval between oocyte retrieval and ICSI improves the outcome of IVF treatment [ 12 , 14 , 32 , 33 ]. However, other studies recommended limiting the cultivation period to prevent post-ovulatory aging of the oocyte [ 34 , 35 ].…”

Section: Discussionmentioning

confidence: 79%

“…However, immature oocytes, which spontaneously extruded PB in vitro, have been successfully used in fertility treatment [ 6 – 10 ]. Cumulative evidence suggests that the developmental potential of late-maturing oocytes could be better exploited if the timing of ICSI is adjusted to their developmental stage [ 2 – 5 , 11 – 14 ].…”

Section: Introductionmentioning

confidence: 99%

Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes

Holubcová¹,

Kyjovská²,

Martonová³

et al. 2019

J Assist Reprod Genet

View full text Add to dashboard Cite

Propose The presence of metaphase II (MII) spindle together with the polar body (PB) indicates completion of oocyte maturation. This study was designed to explore if spindle imaging can be used to optimize timing of intracytoplasmic sperm injection (ICSI). Methods The study involved 916 oocytes from 234 conventionally stimulated ICSI cycles with an unexpectedly poor ovarian response. All PB-displaying oocytes were subjected to polarized light microscopy (PLM) prior to ICSI. When MII spindle was absent in the majority of oocytes, ICSI was postponed and performed after additional spindle imaging. Fertilization, embryo development, and clinical outcome were evaluated with respect to the observed spindle pattern. Results The visible spindle was absent in 32.64% of PB-displaying oocytes. The late-maturing oocytes extruding PB in vitro were less likely to exhibit a spindle signal than in vivo matured MII oocytes (38.86% vs. 89.84%). When fertilization was postponed, 59.39% of initially spindle-negative oocytes developed detectable MII spindle. Spindled eggs had significantly higher developmental potential, and the presence of the spindle has been identified as an independent measure for predicting the formation of the blastocyst. Embryos derived from spindle-positive oocytes also showed a higher chance to implant and develop to term. Notably, 11 children were conceived by finely timed fertilization of late-maturing oocytes which are normally discarded. Conclusions The study confirms the prognostic value of spindle imaging and demonstrates that immature oocytes can be clinically utilized and give rise to live births when the timing of ICSI is adjusted to their developmental stage. Electronic supplementary material The online version of this article (10.1007/s10815-018-1393-0) contains supplementary material, which is available to authorized users.

show abstract

“…Previous substantial studies have examined the optimal time for oocyte denuding and ICSI. While it remains controversial, it has been reported that a 2–6 h interval between oocyte retrieval and ICSI can improve oocyte maturation, fertilization, embryo quality and even pregnancy rate [ 26 – 28 ]. This optimal timing is probably associated with the spindle presence in the oocyte [ 29 , 30 ].…”

Section: Discussionmentioning

confidence: 99%

Perinatal and neonatal outcomes of pregnancies after early rescue intracytoplasmic sperm injection in women with primary infertility compared with conventional intracytoplasmic sperm injection: a retrospective 6-year study

Xiong

Sun

et al. 2020

BMC Pregnancy Childbirth

View full text Add to dashboard Cite

Background: Early rescue intracytoplasmic sperm injection (ICSI) has been used in clinic as appropriate currently. While the outcomes of children born after this method were not well assessed. The purpose of this study was to evaluate the effect of early rescue ICSI on women with primary infertility. Methods: Fresh embryo transfer cycles after rescue (n = 214) and conventional (n = 546) ICSI were retrospectively evaluated from women with primary infertility who underwent their first assisted reproductive technology cycles at our center in 2012-2017. The conventional ICSI group was subdivided into ICSI-1 (semen suitable for in vitro fertilization, IVF) and ICSI-2 (poor semen quality) to minimize bias from differences in semen quality. Pregnancy, delivery and neonatal outcomes were compared between groups. Results: There was a higher rate of polyspermy and a lower rate of top-quality embryos (TQE) on day 3 for oocytes subject to rescue ICSI compared with conventional ICSI. This reduced the total number of TQE and the number of TQE transferred in the rescue ICSI group. There was no significant difference between groups in clinical pregnancy, ongoing pregnancy, early miscarriage and live birth. For pregnant women, gestational age, route of delivery, risk of preterm birth and gestational diabetes mellitus were also comparable. Neonatal outcomes including sex ratio, birth weight, neonatal intensive care unit admission and birth defects were also similar after rescue and conventional ICSI. Moreover, no differences were observed with the different ICSI subgroups. Conclusions: For women with primary infertility who have a high risk of IVF fertilization failure (FF), rescue ICSI provides a safe and efficient alternative to minimize FF after initial IVF, but results in fewer TQE on day 3.

show abstract

Untitled

Abstract: The preincubation of oocytes for at least 2.5 h is beneficial to both IVF and ICSI outcomes by increasing the nuclear maturity of oocytes.

Cited by 42 publications

References 33 publications

Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection

Optimal Timing for Oocyte Denudation and Intracytoplasmic Sperm Injection

Egg maturity assessment prior to ICSI prevents premature fertilization of late-maturing oocytes

Perinatal and neonatal outcomes of pregnancies after early rescue intracytoplasmic sperm injection in women with primary infertility compared with conventional intracytoplasmic sperm injection: a retrospective 6-year study

Contact Info

Product

Resources

About