Kiwifruit Transgenics for Osmotin Gene and Inoculation Tests With Botrytis Cinerea and Cadophora Luteo-Olivacea

Rugini, Eddo; Cristofori, Valerio; Martignoni, D.; Gutiérrez-Pesce, P.; Orlandi, Stefania; Brunori, Elena; Biasi, R.; Muleo, R.; Magro, P.

doi:10.17660/actahortic.2011.913.25

Cited by 3 publications

(3 citation statements)

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“…Furthermore, no damage to DNA was observed in comet assay [ 10 ]. Transgenic kiwifruits ( Actinidia deliciosa ) showed resistance towards postharvest damage, improved shelf life and exhibited tolerance to artificial inoculation of fungal diseases (side rot: Cadophora luteo-olivacea and gray mold: Botrytis cinerea ) [ 48 ]. However, in transgenic mustard plants ( Brassica juncea ), osmotin did not completely provide the resistance against blight ( Alterneria blight ), but delayed the appearance of disease symptoms [ 49 ].…”

Section: Biotic Stress Tolerancementioning

confidence: 99%

Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Bashir

Silvestri

Ahmad

et al. 2020

Plants

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Research on biologically active compounds has been increased in order to improve plant protection against various environmental stresses. Among natural sources, plants are the fundamental material for studying these bioactive compounds as their immune system consists of many peptides, proteins, and hormones. Osmotin is a multifunctional stress-responsive protein belonging to pathogenesis-related 5 (PR-5) defense-related protein family, which is involved in inducing osmo-tolerance in plants. In this scenario, the accumulation of osmotin initiates abiotic and biotic signal transductions. These proteins work as antifungal agents against a broad range of fungal species by increasing plasma membrane permeability and dissipating the membrane potential of infecting fungi. Therefore, overexpression of tobacco osmotin protein in transgenic plants protects them from different stresses by reducing reactive oxygen species (ROS) production, limiting lipid peroxidation, initiating programmed cell death (PCD), and increasing proline content and scavenging enzyme activity. Other than osmotin, its homologous proteins, osmotin-like proteins (OLPs), also have dual function in plant defense against osmotic stress and have strong antifungal activity.

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Section: Biotic Stress Tolerancementioning

confidence: 99%

Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Bashir

Silvestri

Ahmad

et al. 2020

Plants

View full text Add to dashboard Cite

show abstract

“…In addition, osmotin has been used to evaluate its role in post-harvest fruit damage by fungal pathogens. The long-term stored kiwifruits of transgenic kiwi plants expressing tobacco osmotin gene exhibited significant resistance to post-harvest damage by Botrytis cinerea and Cadophora luteo-olivacea, which mainly cause gray mould and skin pitting of kiwifruit, respectively (Rugini et al, 2010). Presently, due to the effects of multiple stress factors in plants, the development of tolerance to individual stresses seems insufficient.…”

Section: Role Of Osmotin In Biotic Stress Tolerancementioning

confidence: 99%

Mechanism of tobacco osmotin gene in plant responses to biotic and abiotic stress tolerance: A brief history

Manghwar¹,

Hussain²

2022

Biocell

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“…Pepper osmotin‐like protein 1 (CaOSM1) is an essential component of defense response, cell death, and oxidative burst (Choi du et al., 2013). Transgenic kiwifruit plants overexpressing a tobacco osmotin gene showed resistance to post‐harvest damage, improved shelf life, and tolerance to fungal diseases (Rugini et al., 2011).…”

Section: Introductionmentioning

confidence: 99%

Acetylation of GhCaM7 enhances cotton resistance to Verticillium dahliae

Zhang

et al. 2023

The Plant Journal

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Protein lysine acetylation is an important post-translational modification mechanism involved in cellular regulation in eukaryotes. Calmodulin (CaM) is a ubiquitous Ca 2+ sensor in eukaryotes and is crucial for plant immunity, but it is so far unclear whether acetylation is involved in CaM-mediated plant immunity. Here, we found that GhCaM7 is acetylated upon Verticillium dahliae (V. dahliae) infection and a positive regulator of V. dahliae resistance. Overexpressing GhCaM7 in cotton and Arabidopsis enhances V. dahliae resistance and knocking-down GhCaM7 makes cotton more susceptible to V. dahliae. Transgenic Arabidopsis plants overexpressing GhCaM7 with mutation at the acetylation site are more susceptible to V. dahliae than transgenics overexpressing the wild-type GhCaM7, implying the importance of the acetylated GhCaM7 in response to V. dahliae infection. Yeast two-hybrid, bimolecular fluorescent complementation, luciferase complementation imaging, and coimmunoprecipitation assays demonstrated interaction between GhCaM7 and an osmotin protein GhOSM34 that was shown to have a positive role in V. dahliae resistance. GhCaM7 and GhOSM34 are co-localized in the cell membrane. Upon V. dahliae infection, the Ca 2+ content reduces almost instantly in plants with downregulated GhCaM7 or GhOSM34. Down regulating GhOSM34 enhances accumulation of Na + and increases cell osmotic pressure. Comparative transcriptomic analyses between cotton plants with an increased or reduced expression level of GhCaM7 and wild-type plants indicate the involvement of jasmonic acid signaling pathways and reactive oxygen species in GhCaM7-enabled disease resistance. Together, these results demonstrate the involvement of CaM protein in the interaction between cotton and V. dahliae, and more importantly, the involvement of the acetylated CaM in the interaction.

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Kiwifruit Transgenics for Osmotin Gene and Inoculation Tests With Botrytis Cinerea and Cadophora Luteo-Olivacea

Cited by 3 publications

References 0 publications

Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Osmotin: A Cationic Protein Leads to Improve Biotic and Abiotic Stress Tolerance in Plants

Mechanism of tobacco osmotin gene in plant responses to biotic and abiotic stress tolerance: A brief history

Acetylation of GhCaM7 enhances cotton resistance to Verticillium dahliae

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