Kisspeptina: efeito na maturação in vitro de ovócitos bovinos

Abstract

This study aimed to evaluate different concentrations of kisspeptin, as well as the interaction of kisspeptin and FSH/LH in vitro maturation and oocyte competence in cattle. In Experiment 1 was determined the minimum concentration of Kisspeptin (Kp) to be used, and in Experiment 2 was evaluated its interection with FSH and LH. The oocytes were collected in a commercial slaughterhouse and only Grade I oocytes were utilized. The oocytes were cultured in TCM-199 medium with bicarbonate plus 10% FBS, sodium pyruvate (22µg/mL), amikacin (83mg/mL), FSH (0.5µg/mL), with different concentrations of Kp, the treatments were: FSH + 0M Kp-10;