Kisspeptin in female reproduction

Lee, Wen-Ling; Lee, Fa‐Kung; Wang, Peng-Hui

doi:10.1097/jcma.0000000000000538

Cited by 3 publications

(3 citation statements)

References 15 publications

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“…ADMA17 can be involved in granulosa cell luteinization and oocyte maturation ( 38 ). Moreover, KISS1 is involved in regulating the gonadotrope axis and the initiation of puberty, timing control of puberty, and regulation of fertility in adulthood mediated by positive and negative feedback regulation on the hypothalamic–pituitary–gonadal (HPG) axis by gonadal steroids ( 56 ). Recently, the protein has been detected to be modulated by leptin and has a role in gonadotropins release ( 57 ).…”

Section: Discussionmentioning

confidence: 99%

Shuttle Transfer of mRNA Transcripts via Extracellular Vesicles From Male Reproductive Tract Cells to the Cumulus–Oocyte Complex in Rabbits (Oryctolagus cuniculus)

et al. 2022

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Semen is known to contain an ovulation-inducing factor (identified as a nerve growth factor, NGF) that shows a significant increase in ovulation after semen deposition in induced ovulatory species. However, the interplay between the male reproductive tract cells and oocyte maturation through messenger RNA (mRNA) cargo is yet to be investigated. Extracellular vesicles (EVs) from the primary culture of rabbit prostate (pEVs), epididymis (eEVs), and testis (tEVs) were isolated to examine their contents for several mRNA transcripts through relative quantitative PCR (RT-qPCR). The expressions of NGF, neurotrophin (NTF3), vascular endothelial growth factor A (VEGFA), A disintegrin and metalloprotease 17 (ADAM17), midkine (MDK), kisspeptin (KISS1), and gonadotrophin-releasing hormone (GNRH1) were examined in isolated EVs. EVs were characterized through transmission electron microscopy. EV uptake by cumulus cell culture was confirmed through microscopic detection of PKH26-stained EVs. Furthermore, the effects of pEVs, eEVs, and tEVs were compared with NGF (10, 20, and 30 ng/ml) supplementation on oocyte in vitro maturation (IVM) and transcript expression. KISS1, NTF3, MDK, ADAM17, GAPDH, and ACTB were detected in all EV types. GNRH1 was detected in tEVs. NGF was detected in pEVs, whereas VEGFA was detected in eEVs. pEVs, eEVs, and 20 ng/ml NGF showed the highest grade of cumulus expansion, followed by tEVs and 10 ng/ml NGF. Control groups and 30 ng/ml NGF showed the least grade of cumulus expansion. Similarly, first polar body (PB) extrusion was significantly increased in oocytes matured with eEVs, pEVs, tEVs, NGF20 (20 ng/ml NGF), NGF10 (10 ng/ml NGF), control, and NGF30 (30 ng/ml NGF). Additionally, the expression of NGFR showed a 1.5-fold increase in cumulus cells supplemented with eEVs compared with the control group, while the expression of PTGS2 (COX2) and NTRK showed 3-fold and 5-fold increase in NGF20-supplemented cumulus-oocyte complexes (COCs), respectively. Oocyte PMP15 expression showed a 1.8-fold increase in IVM medium supplemented with eEVs. Additionally, oocyte NGFR and NTRK expressions were drastically increased in IVM medium supplemented with pEVS (3.2- and 1.6-fold, respectively) and tEVs (4- and 1.7-fold, respectively). This is the first report to examine the presence of mRNA cargo in the EVs of male rabbit reproductive tract cells that provides a model for the stimulation of female rabbits after semen deposition.

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Section: Discussionmentioning

confidence: 99%

Shuttle Transfer of mRNA Transcripts via Extracellular Vesicles From Male Reproductive Tract Cells to the Cumulus–Oocyte Complex in Rabbits (Oryctolagus cuniculus)

et al. 2022

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“…Besides in brain, KiSS/KiSS1R system is widely distributed in peripheral tissues, including gonads, adipose tissue, liver, placenta, pancreas and small bowel, contributing to a wider spectrum of action related to reproduction, fertility, body weight, and energy homeostasis, and cardiovascular system. 7–11 Based on above-mentioned observation, we are happy to introduce the recent article entitled “Role of the kisspeptin/KISS1 receptor system in the testicular development of mice” published in this February issue of the Journal of the Chinese Medical Association . 12…”

mentioning

confidence: 99%

“…6 Besides in brain, KiSS/KiSS1R system is widely distributed in peripheral tissues, including gonads, adipose tissue, liver, placenta, pancreas and small bowel, contributing to a wider spectrum of action related to reproduction, fertility, body weight, and energy homeostasis, and cardiovascular system. [7][8][9][10][11] Based on above-mentioned observation, we are happy to introduce the recent article entitled "Role of the kisspeptin/KISS1 receptor system in the testicular development of mice" published in this February issue of the Journal of the Chinese Medical Association. 12 In the current article, Dr. Chiang et al's 12 group carried out an in vivo observation of cellular pattern of KiSS/KiSS1R in testis tissue of mice model and found that KiSS was identified clearly in the cytoplasm of Leydig cells located adjacent to the seminiferous tubules and KiSS1R was observed in the seminiferous tubules by immunohistochemical staining.…”

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confidence: 99%