Kisspeptin expression in mouse Leydig cells correlates with age

Abstract

Our results suggest that kisspeptin10 does not affect steroidogenesis in adult Leydig cells, but its pattern of expression follows the stages of testicular development. Future studies should determine if kisspeptin regulates testicular development during puberty.

Kisspeptin and Testicular Function—Is It Necessary?

“…KISS1R localisation was not reported. In contrast, Wang et al [42] observed both KISS1 and KISS1R expression in adult mouse Leydig cells.…”

“…Some of these differences observed may be due to variation between species, age differences within the species and the different experimental methods employed [45]. [12,[19][20][21]42], round [12], and elongated spermatids [20]. • KISS1: primordial germ cells [19], and epididymal epithelium [20].…”

“…• kp10: addition of LH had no effect on T production [20,42] [19][20][21]42] and monkeys [18], and KISS1R in Leydig cells of mice [12,42]; 4: KISS1 in primordial germ cells of mice [19] and KISSR in proliferating germ cells of frogs [24]; 5: KISS1/KISS1R in developing spermatids and spermatocytes of mice [12,20], monkeys [17] and frogs [24]; 6: KISS1R in Sertoli cells of frogs [24] and monkeys [17,18]; 7: KISS1R in peritubular myoid cells of frogs [24]; 8: KISS1 in epididymal epithelium of mice [20]. Purple Box: 1: GnRH, GnIH and GnRH-R modulate KISS1 in an autocrine or paracrine manner [19]…”

Kisspeptins: New Local Modulators of Testis Activity

“…In addition, the existence of mutual enhancement between kisspeptin and estradiol may be suggested since Kp-10 increases the expression of ERα/ß and 17β-estradiol increases the expression rate of Gpr54 [19][20] (Figure 2). Similarly than frog, also in mouse the expression profile of kiss1 and ERα fully parallel during postnatal testis development [16]. Consistently, in hypothalamic GT1-7 cell lines kisspeptin induces the expression of ERα and ERβ, whereas estradiol induces that of Gpr54 [22].…”

“…Besides local kisspeptin activity, the secretion of kisspeptin produced by testis has been recently demonstrated in mouse [14]. However, beside the localization and the aforementioned observations, in testis explants and in immortalized or primary Leydig cell culture kisspeptin does not affect steroid biosynthesis [15][16]. By contrast, in rhesus monkey direct effect on testosterone biosynthesis has been demonstrated [17].…”