Kinship and Y-Chromosome Analysis of 7th Century Human Remains: Novel DNA Extraction and Typing Procedure for Ancient Material

Vaněk, Daniel; Saskova, Lenka; Koch, Hubert

doi:10.3325/cmj.2009.50.286

Cited by 42 publications

(41 citation statements)

References 22 publications

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“…Few ancient data are currently available on Y-haplogroups to confirm this hypothesis, but G2a haplotypes have been found in other prehistoric remains; two ancient DNA studies revealed the presence of G2a in the Czech Republic during the seventh century (31) and in a German sample of a central European Neolithic culture (13), whereas this haplogroup is very rare in these places nowadays (32).…”

Section: Resultsmentioning

confidence: 99%

Ancient DNA reveals male diffusion through the Neolithic Mediterranean route

Lacan

Keyser

Ricaut

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

157

160

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The Neolithic is a key period in the history of the European settlement. Although archaeological and present-day genetic data suggest several hypotheses regarding the human migration patterns at this period, validation of these hypotheses with the use of ancient genetic data has been limited. In this context, we studied DNA extracted from 53 individuals buried in a necropolis used by a French local community 5,000 y ago. The relatively good DNA preservation of the samples allowed us to obtain autosomal, Y-chromosomal, and/or mtDNA data for 29 of the 53 samples studied. From these datasets, we established close parental relationships within the necropolis and determined maternal and paternal lineages as well as the absence of an allele associated with lactase persistence, probably carried by Neolithic cultures of central Europe. Our study provides an integrative view of the genetic past in southern France at the end of the Neolithic period. Furthermore, the Y-haplotype lineages characterized and the study of their current repartition in European populations confirm a greater influence of the Mediterranean than the Central European route in the peopling of southern Europe during the Neolithic transition.

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Section: Resultsmentioning

confidence: 99%

Ancient DNA reveals male diffusion through the Neolithic Mediterranean route

Lacan

Keyser

Ricaut

et al. 2011

Proc. Natl. Acad. Sci. U.S.A.

157

160

View full text Add to dashboard Cite

show abstract

“…Improvements in molecular biological technologies in the last decade have helped overcome some of the limitations that restricted DNA analysis to mitochondrial DNA, which is abundant in mammalian cells. In addition, improvements in forensic genetics (e.g., inhibitor-free DNA extraction, multiplex PCR, short STR amplicons and qPCR) have enabled archaeologists to test nuclear DNA, including Y-chromosome STR typing [10,11].…”

Section: Introductionmentioning

confidence: 99%

“…Currently available techniques for DNA analysis using "forensic" procedures [11] can be combined with the classical anthropological examination, advanced imaging and CT scanning techniques [12] and isotope analysis [13] to improve the quality of information that is retrieved from ancient artifacts. A multidisciplinary approach to examining ancient human remains can help verify the DNA analysis results and interpret the findings more precisely.…”

Section: Introductionmentioning

confidence: 99%

Complex Analysis of 700-Year-Old Skeletal Remains found in an Unusual GraveÂ–Case Report

Brzobohata¹

2015

Anthropol

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Aim: The present study was designed to analyze the 700-year-old human remains from an unusual grave using a combined approach that consisted of anthropological, archaeogenetic, genealogical, mass spectrometry, 3-dimensional (3D) modeling and facial reconstruction methods to confirm or reject several hypotheses about the skeletal remains.Methods: DNA was extracted from the skeleton and amplified using autosomal and Y-chromosome human identification short tandem repeat (STR) kits that were designed for forensic use, and sequence data were obtained from hyper variable region I (HVRI) mtDNA sequencing. Elemental mapping and quantification of investigated elements were performed using laser ablation inductively coupled plasma mass spectrometry (LA-ICP-MS). The Computed Tomography (CT) images of the skull were created in a transversal plane, and the scans were used to create 3D geometric models of the skull. A plastic physical model (a cast) of the skull was produced by rapid prototyping technology, and the model was used for sculptural facial approximation of the studied individual. Results:The Y-chromosome haplogroup of the sample was determined to be E1b1b, and the assigned mtDNA haplogroup was H. LA-ICP-MS and geochemical analysis revealed that the individual consumed plants and meats, except pork. Anthropological examination estimated the age of the individual to be between 45-55 years, and we did not find any traces of disablement or physical anomalies. Interestingly, we were able to produce a facial reconstruction according to the skull. Conclusion:Applying a multidisciplinary approach to the examination of the 14th century material enabled us to retrieve new types of information that helped us to interpret the excavated skeletal remains.

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“…The Y-chromosome haplogroupprediction method used utilizes the finding that the pattern of STR alleles in persons with the same haplogroup is similar (Athey, 2005). This allele frequency approach is not so precise as SNP typing (Karafet 2008) but STR typing not only provides higher robustness for compromised samples but also enables the relatedness of male lineages to be compared (Vanek et al 2009). Another possible DNA-typing technology would be Massive Parallel Sequencing (MPS) (e.g.…”

Section: Dna Researchmentioning

confidence: 99%

“…The samples were then transferred to a 50-mL tube and further chemically cleaned by inversion for 30 seconds in 5% commercial bleach, followed by 2-3 washes in distilled water (molecular biology grade), one thorough wash in 96% ethanol (Vanek et al 2009) and left to air-dry.…”

Section: Cleaningmentioning

confidence: 99%

People “on the Margin”: A Medieval Cemetery in Český Brod – Malechov (Central Bohemia)

Frolík¹,

Stránská²,

Votrubová³

et al. 2017

IANSA

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Kinship and Y-Chromosome Analysis of 7th Century Human Remains: Novel DNA Extraction and Typing Procedure for Ancient Material

Abstract: Application of forensic genetics in archaeology enables retrieving new types of information and helps in data interpretation. The number of successfully typed autosomal and Y-STR loci from ancient specimens in this study is one of the largest published so far for aged samples.

Cited by 42 publications

References 22 publications

Ancient DNA reveals male diffusion through the Neolithic Mediterranean route

Ancient DNA reveals male diffusion through the Neolithic Mediterranean route

Complex Analysis of 700-Year-Old Skeletal Remains found in an Unusual GraveÂ–Case Report

People “on the Margin”: A Medieval Cemetery in Český Brod – Malechov (Central Bohemia)

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