Kinin-B2 Receptor Activity in Skeletal Muscle Regeneration and Myoblast Differentiation

Alves, Janaina Maria; Martins, Ana; Lameu, Claudiana; Glaser, Talita; Boukli, Nawal M.; Bassaneze, Vinícius; Dariolli, Rafael; Nascimento, Isis C.; Martins, P.C.M.; Souza, Héllio Danny Nóbrega de; Krieger, José Eduardo; Casarini, Dulce Elena; Sales, Vicência; Pesquero, João Bosco; Ulrich, Henning

doi:10.1007/s12015-018-9850-9

Cited by 13 publications

(10 citation statements)

References 38 publications

(49 reference statements)

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“…The B2 receptor is activated by BK and kallidin. Kinin receptors are expressed in a wide variety of cells and tissues [92–96]. The B1 receptor is not highly expressed under normal conditions, but its expression increases in the context of oxidative stress and the presence of pro‐inflammatory factors.…”

Section: Kallikrein‐kinin System In Glioblastoma‐msc Interactionsmentioning

confidence: 99%

Mesenchymal stem cell‐glioblastoma interactions mediated via kinin receptors unveiled by cytometry

Pillat

Oliveira-Giacomelli

Oliveira³

et al. 2021

Cytometry Pt A

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Glioblastoma (GBM) is one of the most malignant and devastating brain tumors. The presence of highly therapy‐resistant GBM cell subpopulations within the tumor mass, rapid invasion into brain tissues and reciprocal interactions with stromal cells in the tumor microenvironment contributes to an inevitable fatal prognosis for the patients. We highlight the most recent evidence of GBM cell crosstalk with mesenchymal stem cells (MSCs), which occurs either by direct cell–cell interactions via gap junctions and microtubules or cell fusion. MSCs and GBM paracrine interactions are commonly observed and involve cytokine signaling, regulating MSC tropism toward GBM, their intra‐tumoral distribution, and immune system responses. MSC‐promoted effects depending on their cytokine and receptor expression patterns are considered critical for GBM progression. MSC origin, tumor heterogeneity and plasticity may also determine the outcome of such interactions. Kinins and kinin‐B1 and ‐B2 receptors play important roles in information flow between MSCs and GBM cells. Kinin‐B1 receptor activity favors tumor migration and fusion of MSCs and GBM cells. Flow and image (tissue) cytometry are powerful tools to investigate GBM cell and MSC crosstalk and are applied to analyze and characterize several other cancer types.

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Section: Kallikrein‐kinin System In Glioblastoma‐msc Interactionsmentioning

confidence: 99%

Mesenchymal stem cell‐glioblastoma interactions mediated via kinin receptors unveiled by cytometry

Pillat

Oliveira-Giacomelli

Oliveira³

et al. 2021

Cytometry Pt A

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“…Studies have found that myogenic lineage progression is controlled by some myogenic regulatory factors (MRFs), mainly containing myogenic factor 5, myogenic differentiation (MyoD), and myogenin (MyoG) . Many other factors influence these MRFs, such as microRNAs (miRNAs), circular RNAs, and receptor proteins …”

Section: Introductionmentioning

confidence: 99%

“…9,10 Many other factors influence these MRFs, such as microRNAs (miRNAs), circular RNAs, and receptor proteins. [11][12][13][14][15][16] MiRNAs, a class of 21-nucleotide noncoding RNAs, regulate multiple biological processes. 17 MiRNAs regulate target genes by binding to the 3 ′ -untranslated region (3′-UTR) of messenger RNAs (mRNAs) to inhibit translation.…”

Section: Introductionmentioning

confidence: 99%

MicroRNA‐323‐3p promotes myogenesis by targeting Smad2

Qin

Sun

Liu

et al. 2019

J of Cellular Biochemistry

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Skeletal muscle is an important and complex organ with multiple biological functions in humans and animals. Proliferation and differentiation of myoblasts are the key steps during the development of skeletal muscle. MicroRNA (miRNA) is a class of 21‐nucleotide noncoding RNAs regulating gene expression by combining with the 3′‐untranslated region of target messenger RNA. Many studies in recent years have suggested that miRNAs play a critical role in myogenesis. Through high‐throughput sequencing, we found that miR‐323‐3p showed significant changes in the longissimus dorsi muscle of Rongchang pigs in different age groups. In this study, we discovered that overexpression of miR‐323‐3p repressed myoblast proliferation and promoted differentiation, whereas the inhibitor of miR‐323‐3p displayed the opposite results. Furthermore, we predicted Smad2 as the target gene of miR‐323‐3p and found that miR‐323‐3p directly modulated the expression level of Smad2. Then luciferase reporter assays verified that Smad2 was a target gene of miR‐323‐3p during the differentiation of myoblasts. These findings reveal that miR‐323‐3p is a positive regulator of myogenesis by targeting Smad2. This provides a novel mechanism of miRNAs in myogenesis.

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“…A recent study from members of our group shows that BK and B2R possibly play a role in the in vitro differentiation of myoblasts towards skeletal muscle (80). In this work, Alves and collaborators report impaired skeletal muscle regeneration of B2R knockout mice that underwent induced lesion.…”

Section: Figure 3 Schematic Representation Of Signalling Cascade Trimentioning

confidence: 62%

“…Our group has studied, over the years, the mechanisms through which KKS (kininkallikrein system and its receptors) modulates key cellular processes in neurons, such as cell proliferation (83) and differentiation (74,76,77,79,135). Moreover, additional unpublished data from our group (80) and by others (102) suggests that BK aids skeletal muscle regeneration and differentiation. Considering these data and the preponderant effects that BK has on cardiovascular homeostasis, this study has sought understanding a possible impact of BK over the maturation of cardiomyocytes differentiated from hiPSC sources.…”

Section: Effect Of Modulation Of B2r On the Molecular Maturation Of Hmentioning

confidence: 95%

Kinin B2-Receptor in human iPSC differentiation into cardiomyocytes

Góes¹

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Doenças cardiovasculares são responsáveis por quase um terço de todas as mortes globais anualmente, e por isto o sistema cardiovascular é amplamente estudado.Cardiomiócitos derivados a partir de células-tronco pluripotentes induzidas humanas (hiPSC-CM) emergiram como uma promissora tecnologia para modelagem de doenças cardíacas e terapia personalizada. No entanto, desafios acerca de sua maturação funcional e molecular ainda são enfrentados. Além disso, protocolos de diferenciação geralmente levam à obtenção de populações heterogêneas contendo células com fenótipos similares aos de cardiomiócitos nodais, atriais e ventriculares sendo, portanto, inapropriadas para fins terapêuticos. A bradicinina (BK) é um peptídio vasoativo que exerce importantes papeis fisiológicos no sistema cardiovascular, além de ter sido previamente descrita como importante para a diferenciação neuronal, de queratinócitos e de músculo esquelético. Este projeto foi realizado em colaboração com a empresa PluriCell Biotech, uma startup especializada na produção e diferenciação de hiPSC-CM, e buscou (1) caracterizar a expressão gênica e proteíca de marcadores moleculares de maturação e de especificação de subtipos cardíacos durante a diferenciação; (2) avaliar a funcionalidade elétrica de hiPSC-CM por meio da caracterização de seus potenciais de ação (PAs) e (3) Investigar se o progresso da diferenciação de hiPSC-CM é regulado por bradicinina por meio do receptor B2 (B2R). Nossos resultados validaram o modelo que propõe um switch na expressão das isoformas funcionais de troponina I esquelética (ssTnI) e cardíaca (cTnI), durante o desenvolvimento e diferenciação celular, pelo menos parcialmente. Além disso, tempo prolongado em cultura resultou em maiores níveis de expressão do marcador ventricular MLC2v, assim como maiores frequências de PAs com morfologias similares a de cardiomiócitos ventriculares. Análise eletrofisiológica de hiPSC-CM revelam a existência de uma população mista contendo PAs correspondentes aos subtipos nodais, atriais e ventriculares, assim como pronunciada automaticidade e outros atributos típicos de cardiomiócitos imaturos, como baixa amplitude e devagar velocidade de despolarização. Estes resultados são coerentes com os de outros grupos que ainda não foram totalmente bem-sucedidos em diferenciar células cardíacas maduras similares a cardiomiócitos nativos a partir de células-troncos pluripotentes. Após mostrar que as hiPSC-CM expressam receptores B2 funcionais e responsivos, submetemos o receptor a uma ativação crônica com BK 10µM e BK 1µM ou inibição crônica com Firazyr 5µM + BK. Apesar da modulação do B2R não ter interferido de forma negativa no rendimento da diferenciação ou na morfologia celular, análise de expressão gênica e proteica de ssTnI e cTnI e do marcador ventricular MLC2v não revelou resultados significativos em comparação aos controles não-tratados. Isto sugere que a BK não interfere na maturação e especificação de subtipos cardíacos em hiPSC-CM, apesar de não podermos ignorar o fato de que ela poderia estar ...

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Kinin-B2 Receptor Activity in Skeletal Muscle Regeneration and Myoblast Differentiation

Cited by 13 publications

References 38 publications

Mesenchymal stem cell‐glioblastoma interactions mediated via kinin receptors unveiled by cytometry

Mesenchymal stem cell‐glioblastoma interactions mediated via kinin receptors unveiled by cytometry

MicroRNA‐323‐3p promotes myogenesis by targeting Smad2

Kinin B2-Receptor in human iPSC differentiation into cardiomyocytes

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