Kinetische Untersuchung zur Hydratisierung von Acrolein

Arntz, D.; Haas, Th.; Muller, Alfred; Wiegand, N. H.

doi:10.1002/cite.330630714

Cited by 35 publications

(20 citation statements)

References 2 publications

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“…1,3-Propanediol can be produced by either chemical synthesis or microbial fermentation. Current commercial routes to produce 1,3-propanediol are chemical synthetical methods from acrolein or ethylene oxide (Arntz et al 1991;Besson et al 2003;Knifton et al 2004). It has been also reported that glycerol can be converted to 1,3-propanediol via selective dehydroxylation (Wang et al 2003).…”

Section: Introductionmentioning

confidence: 97%

Isolation and characterization of microorganisms able to produce 1,3-propanediol under aerobic conditions

Hao

Lin

Zheng

et al. 2008

World J Microbiol Biotechnol

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Microbial fermentation under strictly anaerobic conditions has been conventionally used for the production of 1,3-propanediol, a key raw material required for the synthesis of polytrimethylene terephthalate (PTT) and other polyester fibers. In the current study, we have identified eight strains of microorganism which are able to produce 1,3-propanediol under aerobic condition. Those strains were isolated from garden soil, which were enriched by culturing in LB medium with glycerol added under aerobic condition. The identities of those strains were established based on their 16S rRNA sequences and physiological characteristics. Results indicated 6 strains are Citrobacter freundii and 2 strains are Klebsiella pneumoniae subsp Penumoniae. One of Klebsiella pneumoniae subsp Penumoniae strains, designated as TUAC01, demonstrated comparable levels of 1,3-propanediol oxidoreductase, glycerol dehydratase and glycerol dehydrogenase activity to the anaerobic microorganisms described in the literature. Accordingly, in larger scales (5 l) fed-batch culture the TUAC01 strain showed a remarkable 1,3-propanediol producing potency under aerobic conditions. 60.1 g/l of 1,3-propanediol was yield after 42 h incubation in an agitating bioreactor; and in air-lift bioreactor 66.3 g/l of 1,3-propanediol was yield after 58.5 h incubation. The aerobic ferment process, reduced the product cost and made the biological method of 1,3-propanediol production more attractive.

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Section: Introductionmentioning

confidence: 97%

Isolation and characterization of microorganisms able to produce 1,3-propanediol under aerobic conditions

Hao

Lin

Zheng

et al. 2008

World J Microbiol Biotechnol

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“…1,3-Propanediol can be produced either by chemical synthesis or by microbial fermentation. Current commercial routes to produce 1,3-propanediol are chemical synthetic methods using acrolein or ethylene oxide as substrates [2,13]. In resent years the advantage of utilizing inexpensive, renewable resource such as glycerol as the substrate, biological route to produce 1,3-propanediol has become increasingly attractive.…”

Section: Introductionmentioning

confidence: 99%

Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01

Hao

Wang

Tian

et al. 2008

J Ind Microbiol Biotechnol

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Glycerol can be biologically converted to 1,3-propanediol, a key raw material required for the synthesis of polytrimethylene terephthalate and other polyester fibers. In 1,3-propanediol synthesis pathway, 3-hydroxypropionaldehyde (3-HPA) was an inhibitory intermediary metabolite. The accumulation of 3-HPA in broth would cause an irreversible cessation of the fermentation process. With the object of reducing 3-HPA level in the fermentation broth, dhaT gene which encodes 1,3-propanediol oxidoreductase (PDOR) was cloned and over expressed in 1,3-propanediol producing bacterium Klebsiella pneumoniae TUAC01. dhaT gene was linked downstream of the ptac promoter in an expressing vector pDK6 to form plasmid pDK-dhaT. The newly formed pDK-dhaT was transformed to K. pneumoniae TUAC01. Under the inducement of IPTG, PDOR was over-expressed when the constructed strain was cultured on an LB medium or a fermentation medium. A 5 L scale-up fermentation experiment was done to test the 3-HPA accumulation in broth, with the initial substrate glycerol 30 g/L; the peak levels of 3-HPA in broth were 7.55 and 1.49 mmol/L for control host strain and the constructed strain, respectively. In 50 g/L initial glycerol experiment, the peak level of 3-HPA in broth was 12.57 and 2.02 mmol/l for the control host strain and the constructed strain, respectively. Thus the fermentation cessation caused by the toxicity of 3-HPA was alleviated in the constructed strain.

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“…This process consists of a three-step reaction: propylene is oxidized to acrolein, acrolein is hydrated to 3-hydroxypropionaldehyde, which then is reduced to PDO (Arntz 1991 The key step in this process is the hydratation of acrolein, where various by-products are generated, and the quality of PDO is dependent on the hydroxylation of acrolein. So a high-performance catalyst which can selectively hydrate acrolein to form 3-hydroxypropionaldehyde is important for the industrial application of this process.…”

Section: Degussa Process Using Propylene As a Feedstockmentioning

confidence: 99%

Microbial 1,3-Propanediol, Its Copolymerization with Terephthalate, and Applications

Liu

Zhou

et al. 2009

Microbiology Monographs

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Poly(trimethylene terephthalate) (PTT) fibers, as a new type of polyester, are characterized by much better resilience and stress/recovery properties than poly(ethylene terephthalate) (PET) and poly(butylene terephthalate) (PBT). PPT chains are much more angularly structured than PET and PBT chains and such chains can be stretched by up to 15% with a reversible recovery (Ward et al. 1976). These properties make PTT highly suitable for uses in fiber, carpet, textile, film, and engineering thermoplastics applications. 1,3-Propanediol (PDO), as one of the polyester raw materials for PTT, has also attracted interest.

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Kinetische Untersuchung zur Hydratisierung von Acrolein

Cited by 35 publications

References 2 publications

Isolation and characterization of microorganisms able to produce 1,3-propanediol under aerobic conditions

Isolation and characterization of microorganisms able to produce 1,3-propanediol under aerobic conditions

Decrease of 3-hydroxypropionaldehyde accumulation in 1,3-propanediol production by over-expressing dhaT gene in Klebsiella pneumoniae TUAC01

Microbial 1,3-Propanediol, Its Copolymerization with Terephthalate, and Applications

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