Kinetin-induced Meiotic-Prophase Acceleration and Stasis in Tradescantia Anthers cultured on Media Deficient in Sugar

Walker, G. W. R.; Dietrich, J.

doi:10.1038/192889b0

Cited by 10 publications

(5 citation statements)

References 8 publications

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“…Clutter and Sussex (1965) suggested that a high level of sucrose brings about an enhancement u of respiratory rates in cultured fern leaves. This suggestion is in agreement with the observations of Walker and Dietrich (1961) who found a stimulation of the division rate in Tradescantia sp. anthers supplied with sucrose.…”

Section: Discussionsupporting

confidence: 92%

In vitro studies on the induction of sporogenous tissue on leaves of cinnamon fern. I. Environmental factors

Harvey¹,

Caponetti²

1972

Can. J. Bot.

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Intact, set III, cinnamon fern cataphyll and frond primordia, which were shown to have no predisposition to fertility in situ, produced sporangia when excised and cultured under sterile conditions in Knudson's medium supplemented with various levels of sucrose and maintained on 11 different regimens of light, darkness, and temperature for 10 weeks. Increasing levels of sucrose resulted in increased fertility under all environmental conditions, but the highest percentage of fertility was obtained under conditions of continuous dark at 26 °C. As the length of the light phase of the photoperiods decreased, a progressive increase in induction of fertile leaves was observed, suggesting that periods of long light exposure are inhibitory to the initiation of sporangia. Conversely, as the light intensity was increased, an inhibition of sporophyll differentiation occurred. Sporangia excised from dark-induced sporophylls and cultured in the light produced viable spores which germinated yielding haploid gametophytes that ultimately produced sporophytes.

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Section: Discussionsupporting

confidence: 92%

In vitro studies on the induction of sporogenous tissue on leaves of cinnamon fern. I. Environmental factors

Harvey¹,

Caponetti²

1972

Can. J. Bot.

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“…Enhancement of meiosis by cytokinin has also been reported in microspore mother cells of Tradescantia paludosa (WALKER and DIETRICH 1961). In this case kinetin stimulates the passage of meiotic cells through prophase and, under some conditions, reduces the time between the second meiotic division and the following mitotic division.…”

Section: Meiosismentioning

confidence: 90%

Functions of Hormones at the Cellular Level of Organization

Evans¹

1984

Hormonal Regulation of Development II

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“…From the experimental standpoint, the reported observations were limited in two ways: (1) Evi-dence for synthesis was obtained by exposing intact anthers to labeled precursors, and isolating the meiotic ceils (microsporocytes) for analysis. Since the level of isotope incorporation by the microsporocytes was much below that of the adjacent somatic tissues, contamination could not be ruled out; (2) Meiotic upsets induced by reagents inhibitory to RNA or protein synthesis could not be unequivocally attributed to interference with macromolecular syntheses in the meiotic cells either because of the possibility that these reagents acted through the adjacent somatic tissues or because similar upsets by other types of reagents had been reported (13).…”

Section: Introductionmentioning

confidence: 99%

SYNTHESIS OF MESSENGER-LIKE RIBONUCLEIC ACID AND PROTEIN DURING MEIOSIS IN ISOLATED CELLS OF TRILLIUM ERECTUM

Hotta

Stern

1963

The Journal of Cell Biology

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The synthcsis of RNA and protein by culturcs of isolated microsporocytes has been demonstratcd. The variation in capacities of such cultures to perform syntheses is a function of meiotic stage and parallels the pattern of changes observed for microsporocytes in situ. A principal feature of this pattern is the induction of syntheses during pachytenc and diplotcne, stages at which the chromosomcs are partly contracted. By use of Actinomycin D, chloramphenicol, pulsc-labeling with P32-phosphate, and nucleotidc analyses of RNA digests, part of the RNA synthesized has becn shown to corrcspond to messenger RNA. Analysis of reaction rates and the ovcrlappings of protcin and RNA synthcsis indicates that the sprcad of cytological events in Trillium is not purely a function of the low temperature at which it occurs but, presumably, ariscs from a complcment of regulatory devices which govern the periodic onset of reactions within the cells. The main conclusion drawn from the whole of these studies is that the sequence of morphological changes associated with chromosomc contraction and movement during meiosis is accompanied by a set of gene transcriptions. Although comparatively few genes are presumed to be active during meiosis, thc action of such gcnes may be cssential to a translation of some of the information cmbodying the mciotic sequence which has been stored in the gcnome in thc course of evolution.

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Kinetin-induced Meiotic-Prophase Acceleration and Stasis in Tradescantia Anthers cultured on Media Deficient in Sugar

Cited by 10 publications

References 8 publications

In vitro studies on the induction of sporogenous tissue on leaves of cinnamon fern. I. Environmental factors

In vitro studies on the induction of sporogenous tissue on leaves of cinnamon fern. I. Environmental factors

Functions of Hormones at the Cellular Level of Organization

SYNTHESIS OF MESSENGER-LIKE RIBONUCLEIC ACID AND PROTEIN DURING MEIOSIS IN ISOLATED CELLS OF TRILLIUM ERECTUM

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