Kinetics study of the localization and quantitation of target antigens of immunoglobulin a antibodies in acquired and congenital toxoplasmosis

Kumolosasi, Endang; Bonhomme, A.; Beorchia, A.; Foudrinier, F.; Marx, C; Pluot, M; Jm, Pinon

doi:10.1007/s004360050136

Cited by 2 publications

(2 citation statements)

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“…The reactivity of the recombinant protein Rop2 196-561 with the acutephase immunoglobulins is not unexpected. Rop2 is a T. gondii protein detected in all three parasite stages (25), and rhoptries are secretory organelles of antigens with IgA reactivity during acute, chronic, and congenital stages in human T. gondii infection (16). Moreover, intestinal and serum IgA antibodies from orally infected mice were shown to react against antigens comigrating with the 55-and 60-kDa rhoptry proteins (7).…”

Section: Discussionmentioning

confidence: 99%

“…Chardes et al (7) used Western blotting to analyze sera, intestinal secretions, and milk from mice orally infected with T. gondii cysts, finding specific IgA reactivity in intestinal secretions against proteins comigrating with p22, p30 (SAG1), p28 (GRA4), and the 55-and 60-kilodalton rhoptry proteins, among others. In addition, the cellular distribution of IgA epitopes on tachyzoites has been analyzed in the course of human acute, chronic, and congenital toxoplasmosis, showing high rhoptry immunolabeling in all cases (16).…”

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confidence: 99%

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Detection of HumanToxoplasma-Specific Immunoglobulins A, M, and G with a RecombinantToxoplasma gondiiRop2 Protein

Martin

Arcavi

Santillán

et al. 1998

Clin Diagn Lab Immunol

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The Toxoplasma gondii rhoptry protein Rop2 was expressed in Escherichia coli as a fusion protein containing 44 kDa of the 55-kDa mature Rop2, supplied with six histidyl residues at the N-terminal end (Rop2196–561). Humoral response during Toxoplasma infection of humans was analyzed by immunoglobulin G (IgG), IgA, and IgM enzyme-linked immunosorbent assay with Rop2196–561 as the antigen substrate. The analyzed sera were divided according to T. gondii-specific serological tests (IgG, IgA, or IgM indirect immunofluorescence and IgA or IgM immunosorbent agglutination assay) as group A (IgG+ IgA− IgM−;n = 35), group B (IgG+ IgA+IgM+; n = 21), group C (IgG+IgA+ IgM−; n = 5), and group D (IgG+ IgA− IgM+;n = 16). Twenty-six T. gondii-seronegative sera from individuals with other infections were also included (group E). Anti-Rop2 IgG antibodies were detected in 82.8% of group A sera and in 97.6% of the sera with acute-phase marker immunoglobulins (groups B, C, and D). The percentage of IgA antibody reactivity against Rop2196–561 was 17.1% in group A, 50% in group D, and 80.8% in groups B and C. The percentage of IgM antibody reactivity was 0% in groups A and C and 62% in groups B and D. Sera from group E failed to show IgA, IgM, or IgG antibody reactivity. Since T. gondii Rop2 elicits a strong humoral response from an early stage of infection, it is suggested that recombinant Rop2196–561 would be suitable for use in diagnostic systems, in combination with other T. gondii antigens, to detect specific IgG, IgA, and IgM antibodies.

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Detection of HumanToxoplasma-Specific Immunoglobulins A, M, and G with a RecombinantToxoplasma gondiiRop2 Protein

Martin

Arcavi

Santillán

et al. 1998

Clin Diagn Lab Immunol

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Performance of a Western Blot Assay to Compare Mother and Newborn Anti- Toxoplasma Antibodies for the Early Neonatal Diagnosis of Congenital Toxoplasmosis

Robert‐Gangneux¹,

Commere²,

Tourte-Schaefer³

et al. 1999

European Journal of Clinical Microbiology & Infectious Dise

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The aim of this study was to retrospectively evaluate the performance of a Western blot assay to compare mother and newborn anti-Toxoplasma gondii antibodies for the early neonatal diagnosis of congenital toxoplasmosis. Since specific anti-Toxoplasma IgM or IgA is detected inconstantly at birth in the neonate, the diagnosis of congenital toxoplasmosis is often delayed until 6-9 months, after IgG titers have been observed persistently. In this study, 81 paired samples from 60 mother/child pairs were tested for IgG and IgM patterns. All mothers had (or were strongly suspected to have) acquired toxoplasmosis during pregnancy. Specific IgM and IgA were simultaneously detected by immunocapture tests, and IgG was titrated. A serological and clinical follow-up of infants was conducted during the first year of life until the diagnosis of congenital toxoplasmosis could be either confirmed or ruled out. Seventeen of the 60 newborns were congenitally infected. Specific IgM or IgA was detected by immunocapture at birth in 76.5% and 70.6% of cord sera from infected neonates, respectively, with an equal specificity of 77.5%. Comparative Western blot allowed the detection of neosynthesized IgG and IgM in the cord blood of 50% and 78.6% of infected infants, respectively, with a specificity of 100%. The combination of IgA and IgM immunocapture tests, the analysis of IgG and IgM Western blot patterns, and the combination of both techniques allowed the detection of 94%, 94%, and 100% of cases within the first 3 months of life, respectively. In conclusion, Western blotting seems to be a useful complementary tool for the early postnatal diagnosis of congenital toxoplasmosis.

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Kinetics study of the localization and quantitation of target antigens of immunoglobulin a antibodies in acquired and congenital toxoplasmosis

Cited by 2 publications

References 15 publications

Detection of HumanToxoplasma-Specific Immunoglobulins A, M, and G with a RecombinantToxoplasma gondiiRop2 Protein

Detection of HumanToxoplasma-Specific Immunoglobulins A, M, and G with a RecombinantToxoplasma gondiiRop2 Protein

Performance of a Western Blot Assay to Compare Mother and Newborn Anti- Toxoplasma Antibodies for the Early Neonatal Diagnosis of Congenital Toxoplasmosis

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