Kinetics Study of Antimicrobial Peptide, Melittin, in Simultaneous Biofilm Degradation and Eradication of Potent Biofilm Producing MDR Pseudomonas aeruginosa Isolates

Khozani, Reyhaneh Shams; Shahbazzadeh, Delavar; Harzandi, Naser; Feizabadi, Mohammad Mehdi; Bagheri, Kamran Pooshang

doi:10.1007/s10989-018-9675-z

Cited by 27 publications

(15 citation statements)

References 42 publications

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“…Another study reported that melittin inhibited biofilm production and destroyed bacterial biofilms [53]. A recent survey implied that melittin was able to penetrate biofilm layers of P. aeruginosa gradually and to kill biofilm-residing bacteria kinetically by disrupting the bacterial membrane [61]. Collectively, these shreds of evidence suggest that melittin can decrease biofilm formation, biofilm biomass, and the viability of bacteria within biofilms in a time-and concentration-dependent manner.…”

Section: Discussionmentioning

confidence: 98%

Pan-Drug Resistant Acinetobacter baumannii, but Not Other Strains, Are Resistant to the Bee Venom Peptide Melittin

et al. 2020

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Acinetobacter baumannii is a prevalent pathogen in hospital settings with increasing importance in infections associated with biofilm production. Due to a rapid increase in its drug resistance and the failure of commonly available antibiotics to treat A. baumannii infections, this bacterium has become a critical public health issue. For these multi-drug resistant A. baumannii, polymyxin antibiotics are considered the only option for the treatment of severe infections. Concerning, several polymyxin-resistant A. baumannii strains have been isolated over the last few years. This study utilized pan drug-resistant (PDR) strains of A. baumannii isolated in Brazil, along with susceptible (S) and extreme drug-resistant (XDR) strains in order to evaluate the in vitro activity of melittin, an antimicrobial peptide, in comparison to polymyxin and another antibiotic, imipenem. From a broth microdilution method, the determined minimum inhibitory concentration showed that S and XDR strains were susceptible to melittin. In contrast, PDR A. baumannii was resistant to all treatments. Treatment with the peptide was also observed to inhibit biofilm formation of a susceptible strain and appeared to cause permanent membrane damage. A subpopulation of PDR showed membrane damage, however, it was not sufficient to stop bacterial growth, suggesting that alterations involved with antibiotic resistance could also influence melittin resistance. Presumably, mutations in the PDR that have arisen to confer resistance to widely used therapeutics also confer resistance to melittin. Our results demonstrate the potential of melittin to be used in the control of bacterial infections and suggest that antimicrobial peptides can serve as the basis for the development of new treatments.

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Section: Discussionmentioning

confidence: 98%

Pan-Drug Resistant Acinetobacter baumannii, but Not Other Strains, Are Resistant to the Bee Venom Peptide Melittin

et al. 2020

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“…The melittin peptide of bee venom exhibit antibacterial activity, prevents MRSA systemic infections and initiates the wound healing process in MRSA-infected mice model (Choi et al, 2015). Khozani et al (2019) studied the efficiency of melittin and found that it degraded about 90-95% of P. aeruginosa biofilm biomass at 50 µg concentrations during 24 h. Human cathelicidin LL-37 inhibits bacterial adhesion and biofilm mass of S. epidermidis ATCC 35984 at a very low concentration (Hell et al, 2010). In addition to anti-biofilm activity, LL-37 exhibits immunomodulatory activity such as cellular recruitment (Tjabringa et al, 2006), enhances host adaptive immune responses (Diamond et al, 2009), and modulates inflammatory responses (Mookherjee et al, 2006).…”

Section: Antimicrobial Peptidesmentioning

confidence: 99%

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Mishra¹,

Panda²,

et al. 2020

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Pathogenic microorganisms and their chronic pathogenicity are significant concerns in biomedical research. Biofilm-linked persistent infections are not easy to treat due to resident multidrug-resistant microbes. Low efficiency of various treatments and in vivo toxicity of available antibiotics drive the researchers toward the discovery of many effective natural anti-biofilm agents. Natural extracts and natural product-based antibiofilm agents are more efficient than the chemically synthesized counterparts with lesser side effects. The present review primarily focuses on various natural anti-biofilm agents, i.e., phytochemicals, biosurfactants, antimicrobial peptides, and microbial enzymes along with their sources, mechanism of action via interfering in the quorum-sensing pathways, disruption of extracellular polymeric substance, adhesion mechanism, and their inhibitory concentrations existing in literature so far. This study provides a better understanding that a particular natural anti-biofilm molecule exhibits a different mode of actions and biofilm inhibitory activity against more than one pathogenic species. This information can be exploited further to improve the therapeutic strategy by a combination of more than one natural anti-biofilm compounds from diverse sources.

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“…Antimicrobial peptides (AMPs) has been a current and highlighted research area because they are not considered drugs and generally do not generate bacterial resistance (Malekkhaiat Häffner & Malmsten, 2019). Generally, AMPs are biologically active with very low concentrations against pathogens such as Escherichia coli, Staphylococcus aureus and Candida albicans (Lorenzón et al, 2012), being also effective in multidrug-resistant (MDR) bacteria that belong to the ESKAPE pathogen group (Shams Khozani et al, 2019; Yin et al, 2020). These types of peptides were also studied in a large group of Gram-positive and Gram-negative bacteria, exhibiting positive results on in vitro (Price et al, 2019) and in vivo (Yang et al, 2019) studies.…”

Section: Introductionmentioning

confidence: 99%

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile²¹)-Ha antimicrobial peptide application as a feed additive

Roque‐Borda

Silva

Crusca

et al. 2021

Preprint

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Microencapsulation is a potential biotechnological tool, which can overcome AMPs instabilities and reduce toxic side effects. Thus, this study evaluates the antibacterial activities of the Ctx(Ile21)-Ha antimicrobial peptide against MDR and non-resistant bacteria, develop and characterize peptide-loaded microparticles coated with HPMCAS and HPMCP. Ctx(Ile21)-Ha microencapsulation was performed by ionic gelation with high-efficiency, maintaining the physical-chemical stability. Ctx(Ile21)-Ha coated-microparticles were characterized, and their hemolytic activity assay demonstrated that hemolysis was decreased up to 95% compared to single molecule. In addition, in vitro release control profile simulating different portions of gastrointestinal tract was performed and showed the microcapsules ability to protect the peptide and release it in the intestine, aimed pathogens location, mainly by Salmonella sp. Therefore, use of microencapsulated Ctx(Ile21)-Ha can be allowed as an antimicrobial controller in monogastric animal production, being a valuable option for molecules with low therapeutic indexes or high hemolytic rates.

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Kinetics Study of Antimicrobial Peptide, Melittin, in Simultaneous Biofilm Degradation and Eradication of Potent Biofilm Producing MDR Pseudomonas aeruginosa Isolates

Cited by 27 publications

References 42 publications

Pan-Drug Resistant Acinetobacter baumannii, but Not Other Strains, Are Resistant to the Bee Venom Peptide Melittin

Pan-Drug Resistant Acinetobacter baumannii, but Not Other Strains, Are Resistant to the Bee Venom Peptide Melittin

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile²¹)-Ha antimicrobial peptide application as a feed additive

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Kinetics Study of Antimicrobial Peptide, Melittin, in Simultaneous Biofilm Degradation and Eradication of Potent Biofilm Producing MDR Pseudomonas aeruginosa Isolates

Cited by 27 publications

References 42 publications

Pan-Drug Resistant Acinetobacter baumannii, but Not Other Strains, Are Resistant to the Bee Venom Peptide Melittin

Pan-Drug Resistant Acinetobacter baumannii, but Not Other Strains, Are Resistant to the Bee Venom Peptide Melittin

Natural Anti-biofilm Agents: Strategies to Control Biofilm-Forming Pathogens

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile21)-Ha antimicrobial peptide application as a feed additive

Contact Info

Product

Resources

About

Alginate-based microparticles coated with HPMCP/AS cellulose-derivatives enable the Ctx(Ile²¹)-Ha antimicrobial peptide application as a feed additive