Kinetics of the Cationic bio-reduction involved in the biosynthesis of silver nanoparticles from Leea Coccinea Leaves

Abstract

The resistance of microorganisms to conventional antimicrobials is one of the most serious health problems that affect not only the human, but also animals and plants, making the search for antimicrobial active ingredients a priority of global research. Green synthesis of antimicrobial silver nanoparticles (AgNPs) is a simple, cost-effective, rapid, reproducible, and environment friendly alternative for which numerous plant species have been reported for this purpose. Previous studies have shown the potential of Leea coccinea leaves in to the biosynthesis of antimicrobial silver nanoparticles. The current research aimed to study the kinetics of the reaction of synthesis of AgNPs by cationic bio-reduction from this botanical bioresource. A technology for the synthesis of AgNPs was established and the influence of operational parameters such as the bio-reduction conditions and the kinetics of the reaction were studied. AgNPs were characterized at different times by UV-VIS spectroscopic method, scanning electron microscopy, determination of particle size, and Z potential through Dynamic Light Scattering technique (DLS). Addition of tensoactive substances was evaluated for the stabilization of the suspension of nanoparticles. The results showed that spherical AgNPs smaller than 100 nm were obtained, which were visually identified by the formation of a dark brown complex with maximum absorption at 470 nm. Kinetic studies demonstrated the influence of the initial plant material on speed and performance, making evident a complex phenomenology with the possible occurrence of parallel reactions, which points to the possible reaction of different reducing compounds contained in this natural source. Addition of surface agents, such as SDS (0,5 %) or maltose (0,5 %), improved the stabilization in the aqueous medium, suggesting the continuation of studies to develop pharmaceuticals formulations based on AgNPs.

Obtención de nanopartículas de plata soportadas en membrana de cabuya (Furcraea andina) y su acción antimicrobiana frente a Staphylococcus aureus.

“…Constituyen la alternativa de prácticas respetuosas con el ambiente, proporcionando una variante promisoria para resolución de problemas en la búsqueda de nuevas sustancias de alto valor biológico que dilucide las incógnitas relacionadas con la seguridad del hombre y su entorno. (47) Los extractos acuosos de cáscara de mandarina variedad común y King en concentraciones entre 1 a 5 % al adicionar sobre soluciones de nitrato de plata de concentración entre 0.001M a 0.0025 M posee efectos de reducción con la producción de nanopartículas de plata en estado de coloidal diferenciados por el rango promedio de longitud de onda entre 420 a 450 nm de absorción de los coloides probablemente de forma esférica. (20) Las condiciones de recubrimiento de fibra de cabuya se atribuyen al uso de nitrato de plata con concentración 0.0025M con inmersión de 60 minutos, y como agente reductor se pueden usar soluciones al 5% de extracto acuoso de cáscara de mandarina variedad común y 3% de variedad King con tiempos de inmersión de 60 minutos.…”

SYNTHESIS AND CHARACTERIZATION OF SILVER NANOPARTICLES MEDIATED BY THE Mentha piperita LEAVES EXTRACT AND EXPLORATION OF ITS ANTIMICROBIAL ACTIVITIES

“…(One of the most prominent study topics is nanotechnology, which significantly contributes to the production of nanomaterials with distinctive features in the disciplines of science and engineering [10]. Currently, nanotechnology is generating a lot of interest and is being promoted as a potential solution to issues in the scientific and technical fields [11]. The manipulation of matter on molecular, supramolecular and an atomic scale is known as nanotechnology.…”