Kinetics of styrene biodegradation by Pseudomonas sp. E-93486

Abstract

The research into kinetics of styrene biodegradation by bacterial strain Pseudomonas sp. E-93486 coming from VTT Culture Collection (Finland) was presented in this work. Microbial growth tests in the presence of styrene as the sole carbon and energy source were performed both in batch and continuous cultures. Batch experiments were conducted for initial concentration of styrene in the liquid phase changed in the range of 5–90 g m−3. The Haldane model was found to be the best to fit the kinetic data, and the estimated constants of the equation were: μm = 0.1188 h−1, KS = 5.984 mg l−1, and Ki = 156.6 mg l−1. The yield coefficient mean value for the batch culture was 0.72 gdry cells weight (gsubstrate)−1. The experiments conducted in a chemostat at various dilution rates (D = 0.035–0.1 h−1) made it possible to determine the value of the coefficient for maintenance metabolism md = 0.0165 h−1 and the maximum yield coefficient value . Chemostat experiments confirmed the high value of yield coefficient observed in the batch culture. The conducted experiments showed high activity of the examined strain in the styrene biodegradation process and a relatively low sensitivity to inhibition of its growth at higher concentrations of styrene in the solution. Such exceptional features of Pseudomonas sp. E-93486 make this bacterial strain the perfect candidate for technical applications.

Agro-process intensification: soilborne micro-bioreactors with nitrogen fixing bacterium Azospirillum brasilense as self-sustaining biofertiliser source for enhanced nitrogen uptake by plants

“…Styrene monomer is biodegradable and is consequently excreted in urine from the body as water soluble metabolites [ 47 ]. The metabolic pathways, genetic and physiological aspects of degradation have been well established [48][49][50]. Although several large epidemiological studies (see, for example, [ 51 ]) did not support a link between styrene and cancer, according to the US National Toxicology Programme styrene is reasonably anticipated to be a human carcinogen.…”

Start a Research on Biopolymer Polyhydroxyalkanoate (PHA): A Review

“…An exception is P. oleovorans (ATCC 8062) where its cultivation on 4-hydroxyhexanoic acid resulted in a copolymer predominated by 3HB (92.4 mol%) [56]. Pseudomonads are also well-known for their bioremediation properties including the biodegradation recalcitrant and/or toxic aromatic carbon substrates [98], and have been successfully applied in the treatment of contaminated effluents, exhaust gas and soils [99][100][101]. Recent studies demonstrated that aromatic-degraders P. putida F1 (DSM 6899), P. putida mt-2 (NCIMB 10432), and P. putida CA-3 (NCIMB 41162) could bioconvert toxic pollutants benzene, toluene, ethylbenzene, xylene (BTEX) and styrene to mcl-PHA [86] [76,77,80], which offers the potential benefit to off-set waste treatment cost through PHA recovery.…”

Bioconversion of Styrene to Poly(hydroxyalkanoate) (PHA) by the New Bacterial Strain <i>Pseudomonas putida</i> NBUS12

“…Significant efforts have been made to isolate styrene-degrading bacterial strains, such as members from Pseudomonas , Rhodococcus , Bacillus , and Xanthobacter (41), and apply them to the bioremediation of styrene-contaminated effluents, off-gas, and soils (7, 8, 12). …”