Kinetics of spermatogenesis in mammals: seminiferous epithelium cycle and spermatogonial renewal.

Clermont, Y.

doi:10.1152/physrev.1972.52.1.198

Cited by 1,213 publications

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“…In the mouse there are about 10 8 cells in the testis and approximately 2 × 10 4 are thought to be stem cells (Meistrich & van Beek, 1993;Tegelenbosch & de Rooij, 1993). Thus, most germ cells in the seminiferous tubules are mitotic spermatogonia, spermatocytes undergoing meiosis, and highly differentiated terminal stages such as spermatids (Huckins, 1971;Clermont, 1972). From a single stem cell in the rat, theoretically 1024 primary spermatocytes can be formed, but considerably fewer are produced because of cell loss (Russell et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Culture of mouse spermatogonial stem cells

et al. 1998

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Spermatogenesis occurs within the seminiferous tubules of mammals by a complex process that is highly organized, extremely efficient and very productive. At the foundation of this process is the spermatogonial stem cell that is capable of both self-renewal and production of progeny cells, which undergo differentiation over a period of weeks to months in order to generate mature spermatozoa. It had been thought that germ cells survive only a brief period in culture, generally less than a few weeks. However, an accurate assessment of the presence of spermatogonial stem cells in any cell population has only recently become possible with development of the spermatogonial transplantation technique. Using this technique, we have demonstrated that mouse spermatogonial stem cells can be maintained in culture for approximately 4 months and will generate spermatogenesis following transplantation to the seminiferous tubules of an appropriate recipient. Extensive areas of cultured donor cell-derived spermatogenesis are generated in the host, and production of mature spermatozoa occurs. Cultivation of the testis cells on STO feeders is beneficial to stem cell survival. These results provide the first step in establishing a system that will permit spermatogonial stem cells to be cultivated and their number increased in vitro to allow for genetic modification before transplantation to a recipient testis.

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Section: Introductionmentioning

confidence: 99%

Culture of mouse spermatogonial stem cells

et al. 1998

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“…The round haploid spermatids then change their shape dynamically into sperm 2 . The duration of this process from SSCs to sperm takes 35 and 64 days in mice and humans, respectively 3 . The process is not germ cell-autonomous, but requires orchestrated support from the micro-environmental milieu produced by surrounding somatic cells, especially Sertoli and Leydig cells 4,5 .…”

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In vitro production of fertile sperm from murine spermatogonial stem cell lines

et al. 2011

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spermatogonial stem cells (ssCs) are the only stem cells in the body that transmit genetic information to the next generation. The long-term propagation of rodent ssCs is now possible in vitro, and their genetic modification is feasible. However, their differentiation into sperm is possible only under in vivo conditions. Here we show a new in vitro system that can induce full spermatogenesis from ssC lines or any isolated ssCs. The method depends on an organ culture system onto which ssCs are transplanted. The settled ssCs form colonies and differentiate up into sperm. The resultant haploid cells are fertile, and give rise to healthy offspring through micro-insemination. In addition, the system can induce spermatogenesis from ssCs that show spermatogenic failure due to a micro-environmental defect in their original testes. Thus, an in vitro system is established that can be used to correct or manipulate the micro-environmental conditions required for proper spermatogenesis from murine ssC lines.

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“…Les differents types cellulaires qui caracterisent la spermatogenese constituent dans les tubes seminiferes des associations bien definies qui forment le << cycle de I'epithelium seminifere >> dans lequel une sequence cellulaire est repetee & I'infini [1]. Le cycle de I'epithelium seminifere est un phenomene histologique dynamique qui prend place avec le temps en un endroit donne de I'epithelium seminifere.…”

Section: Rappel De La Spermatogeneseunclassified

Histologie testiculaire et études méiotiques dans les stérilités de type non obstructif

Ghalamoun-Slaimi

Guichaoua

2006

Androl.

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Kinetics of spermatogenesis in mammals: seminiferous epithelium cycle and spermatogonial renewal.

Cited by 1,213 publications

References 0 publications

Culture of mouse spermatogonial stem cells

Culture of mouse spermatogonial stem cells

In vitro production of fertile sperm from murine spermatogonial stem cell lines

Histologie testiculaire et études méiotiques dans les stérilités de type non obstructif

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