Kinetics of Phenol Biodegradation by Heavy Metal Tolerant Rhizobacteria Glutamicibacter nicotianae MSSRFPD35 From Distillery Effluent Contaminated Soils

Abstract

Biodegradation of phenol using bacteria is recognized as an efficient, environmentally friendly and cost-effective approach for reducing phenol pollutants compared to the current conventional physicochemical processes adopted. A potential phenol degrading bacterial strain Glutamicibacter nicotianae MSSRFPD35 was isolated and identified from Canna indica rhizosphere grown in distillery effluent contaminated sites. It showed high phenol degrading efficiency up to 1117 mg L –1 within 60 h by the secretion of catechol 1,2-dioxygenase via ortho intradial pathway. The strain MSSRFPD35 possess both the catechol 1,2 dioxygenase and catechol 2,3 dioxygenase coding genes that drive the ortho and meta pathways, but the enzymatic assay revealed that the strain cleaves catechol via ortho pathway. Haldane’s kinetic method was well fit to exponential growth data and the following kinetic parameter was obtained: μ ∗ = 0.574 h –1 , K i = 268.1, K s = 20.29 mg L –1 . The true μ max and S m were calculated as 0.37 h –1 and 73.76 mg L –1 , respectively. The Haldane’s constant values were similar to earlier studies and healthy fitness depicted in correlation coefficient value R 2 of 0.98. Phenol degrading kinetic’s was predicted using Haldane’s model as q max 0.983, K i ′ 517.5 and K s ′ 9.152. Further, MSSRFPD35 was capable of utilizing different monocyclic and polycyclic aromatic hydrocarbons and to degrade phenol in the presence of different heavy metals. This study for the first time reports high phenol degrading efficiency of G. nicotianae MSSRFPD35 in the presence of toxic heavy metals. Thus, the strain G. nicotianae MSSRFPD35 can be exploited for the bioremediation of phenol and its derivatives polluted environments, co-contaminated with heavy metals.

Bioremediation by Cupriavidus metallidurans Strain MSR33 of Mercury-Polluted Agricultural Soil in a Rotary Drum Bioreactor and Its Effects on Nitrogen Cycle Microorganisms

“…Our study is the first report describing mercury-resistant strains from the Glutamicibacter , Planomicrobium and Bergeyella genera. Nevertheless, heavy metal resistance in Glutamicibacter and Planomicrobium strains were described [ 74 , 75 , 76 ]. Mercury-tolerant Bacillus was isolated from mercury-contaminated soils, water, sediments, and High-Arctic snow and freshwater [ 63 , 69 , 71 , 73 , 77 , 78 , 79 ].…”

Deciphering the Multi-Dimensional Abilities of Indigenous Bacteria Enterobacter Cloacae Isolated from Arsenic Contaminated Industrial Sites

“…The speci c growth rate (µ) was calculated from the slope of a semi-logarithmic plot of dry biomass ln(X/X 0 ) vs. time (Duraisamy et al 2020).…”

Utilization of Phenol as Carbon Source by the Thermoacidophilic Archaeon Saccharolobus solfataricus P2 Is Limited by Oxygen Supply and the Cellular Stress Response

“…Additionally, phenolic compounds can result from degradation of natural organic matter in the environment, e.g., from the decomposition of plants and animals ( Anku et al, 2017 ), and are thus ubiquitous in the environment. A number of mesophilic bacteria and yeasts can degrade phenol, e.g., Acinetobacter radioresistens , Glutamicibacter nicotianae , and Candida subhashii ( Filipowicz et al, 2017 ; Duraisamy et al, 2020 ; Liu et al, 2020 ). The mechanism of phenol degradation in mesophilic microorganisms has been thoroughly studied because of their application in bioremediation.…”