Kinetics of oxidation of glutathione by an octahedral cobalt(III) complex with phenolate–amide–amine coordination

Nayak, Suprava; Reddy, K. V.; Dash, Anadi C.

doi:10.1007/s11243-013-9787-4

Cited by 2 publications

(3 citation statements)

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“…These latter complexation reactions were significantly slower than the electron transfer reactions [201]. In another study, the reduction of the cobalt(III) complex, [Co III (HL)]•9H 2 O (where H4L = 1,8-bis(2-hydroxybenzamido)-3,6-diazaoctane) by GSH was studied by conventional spectrophotometry in acidic media [202]. The final products were determined to be the corresponding Co(II) complex and GSSG [202].…”

Section: Spectroscopic Studies In Water Pbs Buffer and An Aqueous Smentioning

confidence: 99%

Synthesis, characterization, DNA binding, topoisomerase inhibition, and apoptosis induction studies of a novel cobalt(III) complex with a thiosemicarbazone ligand

Beebe¹,

Celestine

Bullock

et al. 2020

Journal of Inorganic Biochemistry

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× 10 5 and 1.6 × 10 4 M −1 , respectively). Topoisomerase inhibition assays and cleavage studies proved that complex 4 was an efficient catalytic inhibitor of human topoisomerases I and IIα. Based upon the results obtained from the 3-(4,5-dimethylthiazol-2-yl)-5-(3carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) assay on 4T1-luc metastatic mammary breast cancer cells (IC 50 = 34.4 ± 5.2 μM when compared to IC 50 = 13.75 ± 1.08 μM for the control, cisplatin), further investigations into the molecular events initiated by exposure to complex 4 were investigated. Studies have shown that complex 4 activated both the apoptotic and autophagic signaling pathways in addition to causing dissipation of the mitochondrial membrane potential (ΔΨ m). Furthermore, activation of cysteine-aspartic proteases3 (caspase 3) in a time-and concentration-dependent manner coupled with the ΔΨ m , studies implicated the intrinsic apoptotic pathway as the major regulator of cell death mechanism.

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Section: Spectroscopic Studies In Water Pbs Buffer and An Aqueous Smentioning

confidence: 99%

Synthesis, characterization, DNA binding, topoisomerase inhibition, and apoptosis induction studies of a novel cobalt(III) complex with a thiosemicarbazone ligand

Beebe¹,

Celestine

Bullock

et al. 2020

Journal of Inorganic Biochemistry

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“…The bands at 1346 cm -1 (w) and 1305 cm -1 are ascribed to the O-H bending and νstr(C=O) of co-ordinated phenolic groups and those at 833 and 750 cm -1 are assigned to the (1,2)disubstituted aromatic moiety. The bands at 1091, 1026, 914 cm -1 (CH2-rocking) and prominent peaks at 1487 and 1383 cm -1 due to CH2 deformation vibration are concomitant with the unsymmetrical array of diene spacer unit [32].…”

Section: Esi-ms Spectramentioning

confidence: 95%

“…In continuation of our earlier work on the redox reaction of GSH and cobalt(III) complex of hexadentate phenol-amide ligand [32], we report herein the synthesis and characterization of a Co(III) complex, [Co III (HL)(H2O)] with a pentadentate ligand 1,5-bis(2-hydroxybenzamido)-3-aza pentane (H3L) and its redox activity with GSH. The objective behind this study is to explain the ligand substitution dynamics of [Co III (HL)] with GSH, to distinguish the reactive species, characterize reaction products and to establish the rate law and construe a plausible mechanism.…”

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confidence: 96%

Synthesis and Characterization of Cobalt(III) Complex of 1,5-bis(2-Hydroxybenzamido)-3- aza pentane and its Interaction with Glutathione: A Kinetic and Spectrophotometric Study

Aravind

Brahma

Nayak³

2019

Asian J. Chem.

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A new octahedral cobalt(III) complex of 1,5-bis(2-hydroxybenzamido)-3-aza pentane was synthesized and characterized by elemental analysis, UV-visible, IR, mass spectrometry and thermal analysis methods. Oxidative behaviour of the complex towards the physiologically abundant tripeptide, glutathione(GSH) was studied by UV-visible and FT-NMR techniques. Kinetics of electron transfer between the complex and GSH was studied at 25.0 ≤ t/°C ≤ 45.0, 0.025 ≤ [H+]/mol dm-3 ≤ 0.20 and I = 1.0 mol dm-3 (NaClO4). NMR spectra of the reaction mixture were taken as a function of time. Changes observed in the NMR-spectrum during the period of scanning are the initial disappearance of the fine structure and subsequent broadening of the signals of GSH protons. Peaks at (δ, ppm): 2.93 and 2.98, characteristics of Cys-CH2 δ protons in GSH get disappear slowly and new peaks develop at (δ, ppm): 3.33 and 3.38, corresponding to resonating frequencies of Cys-CH2 δ protons in GSSG. Likewise, the peak at (δ, ppm):2.93, characteristic of Cys-CH2 δ proton in GSH is replaced by the resurgence of two peaks at (δ, ppm): 4.3 and 4.1 ppm, commensurate with the resonating frequencies of same set of protons as that of GSH but in a dimeric environment. The time dependent NMR simulation of the reaction reveals that GSH reduces the CoIII moiety successfully, while it gets converted into the dimer, GSSG. The relatively fast transformation of GSH to GSSG as evident from both electronic spectra and NMR studies together with the activation parameters obtained from the temperature dependence of reaction rate suggest that there is no involvement of any bridging group for electron transfer and the redox process may be taking place via outer sphere mechanism.

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Kinetics of oxidation of glutathione by an octahedral cobalt(III) complex with phenolate–amide–amine coordination

Cited by 2 publications

References 21 publications

Synthesis, characterization, DNA binding, topoisomerase inhibition, and apoptosis induction studies of a novel cobalt(III) complex with a thiosemicarbazone ligand

Synthesis, characterization, DNA binding, topoisomerase inhibition, and apoptosis induction studies of a novel cobalt(III) complex with a thiosemicarbazone ligand

Synthesis and Characterization of Cobalt(III) Complex of 1,5-bis(2-Hydroxybenzamido)-3- aza pentane and its Interaction with Glutathione: A Kinetic and Spectrophotometric Study

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