Kinetics of osmoregulation in the crab Carcinus maenas

Siebers, D.; Lucu, Čedomil; Sperling, K. R.; Eberlein, K.

doi:10.1007/bf00366739

Cited by 106 publications

(26 citation statements)

References 28 publications

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“…2). These data correspond to increased metabolic activity in shore crabs inhabiting dilute media, indicated by increased 02 uptake and elevated levels of serum protein and hemocyanin (Theede, 1964;Siebers et al, 1972). In this context, thickening of the gill epithelia due to surface enlargements was described for the fiddler crab Uca pugilator upon transfer to low salinity by Graszynski & Drews (1981).…”

Section: Activation and Inhibition Of Na-k-atpase By Namentioning

confidence: 86%

“…It is known that after sudden environmental salinity change, inorganic hemolymph constituents and osmotic pressure in C. maenas attain their new steady-state levels within 1 to 2 days {Siebers et al, 1972). This relatively fast response may also be based on the substrate activation and inhibition properties of the monovalent cation pump.…”

Section: Activation and Inhibition Of Na-k-atpase By Namentioning

confidence: 99%

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Regulation of sodium in the shore crabCarcinus maenas, adapted to environments of constant and changing salinities

Siebers

Winkler

Leweck

et al. 1983

Helgolander Meeresunters

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The activity of Na-K-ATPase was determined in the posterior gills of the shore crab Carcinus maenas during a period following transfer from 35 to 10 %o salinity and vice versa at 15 °C. After transfer from high to low salinity, Na-K-ATPase activity increased from 3.2 to 7.0 ~tmoles Pimg protein -1 h-1 within a period of 2 to 3 weeks. Transfer of crabs from low to high salinity resulted in reduction of activity from 7.4 to 4.5 ~moles Pimg protein -1 h -1 within about the same period. The relatively slow response following salinity change indicates that the amounts of Na-K-ATPase in the gills may play a role in hyperionic Na regulation in relatively constant brackish-water environments. Instant responses to salinity result from activation and inhibition of Na-K-ATPase activity by Na. Gill Na-K-ATPase is activated by the Na concentration of the incubation medium to attain a steep maximum at about 75 mM Na, which corresponds to the lowest environmental Na levels tolerated by C. maenas equivalent to a salinity of ca 6 %°. Activity greatly decreased towards higher Na levels, equivalent to the salinity of normal sea water, at which hyperregulation no longer occurs. Selective addition of either Na or C1 to brackish water of 9 %0 S resulted in effective hyperregulation of the non-increased ion, and passive distribution between medium and blood of the increased ion. These data indicate that under appropriate conditions the normally coupled transport of Na and Ct may be uncoupled and take place independently of each other.

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Section: Activation and Inhibition Of Na-k-atpase By Namentioning

confidence: 86%

Section: Activation and Inhibition Of Na-k-atpase By Namentioning

confidence: 99%

Regulation of sodium in the shore crabCarcinus maenas, adapted to environments of constant and changing salinities

Siebers

Winkler

Leweck

et al. 1983

Helgolander Meeresunters

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“…Finally, a defined amount of trimethylsilyl-mannitol was added as internal standard (Clamp et al, 1967;Vance and Sweeley, 1967;Bhakti et al, 1970). For gas chromatography of the carbohydrate derivates at different temperature programs see Siebers et al (1972).…”

Section: Analysis Of Dissolved Neutral Carbohydratesmentioning

confidence: 99%

The Development of a Natural Plankton Population in an Outdoor Tank with Nutrient-Poor Sea Water. II. Changes in Dissolved Carbohydrates and Amino Acids

Brockmann¹,

Eberlein²,

Junge³

et al. 1979

Mar. Ecol. Prog. Ser.

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Interrelations between plankton communities and dissolved carbohydrates and amino acids were investigated under near-natural conditions in sea water enclosed in plastic tanks. In summer 1972 the development of a natural plankton population was followed in a 3-m3 plastic tank for 28 d. In the course of this experiment, concentrations of dissolved neutral carbohydrates and free amino acids were determined. Results are in the range of published data for the open sea with respect to concentrations (0.2-2.5 pnoles dm-3 total sugar; 0.2-3.1 pnoles dm3 total amino acids) and qualitative composition. A plankton succession was observed during the experiment; this was accompanied by distinct alterations in the concentrations of dissolved amino acids and carbohydrates. Glucose and lysine occurred in highest concentrations. Maximum rate of increase was 29 nmoles dm3 h-' for glucose, and 25 nmoles dm3 h-' for lysine. The rates of decrease are in the same range as bacterial uptake rates determined by various authors employing tracer methods. Numerous positive, highly significant correlations suggest heteropolysaccharides as one source of individual carbohydrates. Relations between certain species within the plankton succession and occurrence of dissolved organic substances were observed. Significant positive correlations existed between glucose and diatoms as well as between glucose, galactose, mannose, arabinose and ribose and phytoplankton biomass. There were also several significant positive correlations of amino acids, especially of valine, leucine and isoleucine with other biological parameters.

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“…Further enhancement of nutrients had no effect. The nutrient solution was composed according to the hemolymph concentration of the six most frequent substances (amino acids and glucose) in (7. maenas (Siebers et al, 1972). The results obtained indicate that the isolated gill organ can generate potential differences of active nature, utilizing its own sources of energy or by additional use of externally available nutrients.…”

Section: Potential Differences Across Isolated Perfused Anterior and mentioning

confidence: 85%

“…For experiments testing the influence of added nutrients on establishment of the potential difference, artificial nutrients were dissolved in 50 % sea water according to Siebers et al (1972). The final concentrations were {raM): NH4 C1 100, glucose 100, glycine 30, proline 20, taurine 20, lysine 20.…”

Section: Potential Differences Of Isolated Perfused Gillsmentioning

confidence: 99%

The role of the transbranchial potential difference in hyperosmotic regulation of the shore crabCarcinus maenas

Winkler

1986

Helgolander Meeresunters

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When isolated gills of the shore crab Carcinus maenas were bathed and perfused with identical solutions on both sides (50 % sea water), a spontaneous transepithelial potential difference (PD) of some millivolts (hemolymph side negative) was established. This PD is of active nature and requires the metabolism of the living cell, since it uses its own sources of energy in addition to organic nutrients offered in the flow of artificial hemolymph. Addition of sodium cyanide and dinitrophenole to bathing and perfusion medium resulted in reversible breakdown of PDs in a concentration-dependent mode. In posterior gills of C. maenas, the potential differences were more negative compared to data measured in anterior gills of the same individuals. These results are correlated with higher specific activities of Na-K-ATPase in posterior gills. Experiments with triamterene indicate that sodium uptake in C. maenas is sensitive to this diuretic drug, when applied on the apical side of the epithelial cell. The results obtained show that active uptake of sodium from medium to blood across the g~s is performed by a complex mechanism including participation of several basal and apical transport steps.

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Kinetics of osmoregulation in the crab Carcinus maenas

Cited by 106 publications

References 28 publications

Regulation of sodium in the shore crabCarcinus maenas, adapted to environments of constant and changing salinities

Regulation of sodium in the shore crabCarcinus maenas, adapted to environments of constant and changing salinities

The Development of a Natural Plankton Population in an Outdoor Tank with Nutrient-Poor Sea Water. II. Changes in Dissolved Carbohydrates and Amino Acids

The role of the transbranchial potential difference in hyperosmotic regulation of the shore crabCarcinus maenas

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