Abstract:We studied the effect of optical clearing (OC) by glycerol, Omnipaque TM (iohexol), and polyethylene glycol on human skin in vivo using a portable Raman spectroscopy system. Kinetic curves were determined for different concentrations of these optical clearing agents (OCAs). The three agents enhanced OC, showing non-monotonic behavior for serial concentrations. The dehydration mechanism also caused a decay in OC during the initial 10−20 min. Furthermore, characteristic Raman signals could be used to identify di… Show more
“…These results demonstrated the apparently enhanced effect of glycerol on the molecular bands of the laser induced plasma. After being immersed with glycerol, the tissue dehydration was induced by the osmotic properties of glycerol [14]. According to Fick's second law of diffusion, due to tissue dehydration, the agent concentration decreases for glycerol and increases for water linearly from the surface to deeper sites of the tissue [15].…”
We studied the effect of optical clearing (OC) by glycerol on laser-induced tissue plasma using the immersion method. The results demonstrated the apparently enhanced effect of glycerol on the molecular spectra of the laser induced plasma. The OC is more sensitive to the molecular bands than atomic lines. After tissue immersion in the glycerol, the electron density of tissue plasma is decreased. The laser plasma temperature of the glycerol treated tissue is higher than for virgin fresh tissue. The tissue plasma after the glycerol application is still in the local thermal equilibrium plasma state. This work presents a new perspective for OC application that can extend from tissue better imaging quality to improvement of laser plasma generation.
“…These results demonstrated the apparently enhanced effect of glycerol on the molecular bands of the laser induced plasma. After being immersed with glycerol, the tissue dehydration was induced by the osmotic properties of glycerol [14]. According to Fick's second law of diffusion, due to tissue dehydration, the agent concentration decreases for glycerol and increases for water linearly from the surface to deeper sites of the tissue [15].…”
We studied the effect of optical clearing (OC) by glycerol on laser-induced tissue plasma using the immersion method. The results demonstrated the apparently enhanced effect of glycerol on the molecular spectra of the laser induced plasma. The OC is more sensitive to the molecular bands than atomic lines. After tissue immersion in the glycerol, the electron density of tissue plasma is decreased. The laser plasma temperature of the glycerol treated tissue is higher than for virgin fresh tissue. The tissue plasma after the glycerol application is still in the local thermal equilibrium plasma state. This work presents a new perspective for OC application that can extend from tissue better imaging quality to improvement of laser plasma generation.
“…The mean alveolar number in humans is 274-790 million with the mean size of a single alveolus of 4.2 × 10 6 μm 3 or approximately 200 μm in diameter (Ochs et al 2004). Air-filled lungs present significant challenges for optical imaging including optical coherence tomography (OCT) (Quirk et al 2014;Ding et al 2021), confocal and two-photon microscopy, and Raman spectroscopy, because of the large refractive-index mismatch between alveoli walls and the enclosed air-filled region, major challenges are associated with the improvement of imaging resolution even further to the subcellular level (Yu et al 2021;Lin et al 2020;Yanina et al 2020;Jaafar et al 2021;Genina 2022).…”
Optical clearing of the lung tissue aims to make it more transparent to light by minimizing light scattering, thus allowing reconstruction of the three-dimensional structure of the tissue with a much better resolution. This is of great importance for monitoring of viral infection impact on the alveolar structure of the tissue and oxygen transport. Optical clearing agents (OCAs) can provide not only lesser light scattering of tissue components but also may influence the molecular transport function of the alveolar membrane. Air-filled lungs present significant challenges for optical imaging including optical coherence tomography (OCT), confocal and two-photon microscopy, and Raman spectroscopy, because of the large refractive-index mismatch between alveoli walls and the enclosed air-filled region. During OCT imaging, the light is strongly backscattered at each air-tissue interface, such that image reconstruction is typically limited to a single alveolus. At the same time, the filling of these cavities with an OCA, to which water (physiological solution) can also be attributed since its refractive index is much higher than that of air will lead to much better tissue optical transmittance. This review presents general principles and advances in the field of tissue optical clearing (TOC) technology, OCA delivery mechanisms in lung tissue, studies of the impact of microbial and viral infections on tissue response, and antimicrobial and antiviral photodynamic therapies using methylene blue (MB) and indocyanine green (ICG) dyes as photosensitizers.
Background Objectives: Newly developed in vivo skin and skull optical clearing techniques can greatly improve the optical imaging performance, showing great advantages and clinical prospects. However, there is a poor understanding of in vivo optical clearing-induced changes in the skin and skull. Materials and Methods: Here, we employed in vivo skin/skull optical clearing techniques to improve the optical coherence tomography (OCT) imaging quality. And we also used polarization-sensitive OCT to monitor the dynamic changes in the polarization characteristics of the skin and skull during in vivo optical clearing processes. Two-photon imaging was used to evaluate changes in tissue barrier function and structure. Additionally, Raman spectra were employed for assessing the changes of each component in the skin and skull before and after optical clearing treatment. Results: The results indicated that the polarization states of the skin and skull were altered with the usages of optical clearing agents. And the barrier permeability and collagen fiber distribution of them became disordered. Furthermore, the Raman spectra of tissue demonstrated that the applications of in vivo tissue optical clearing methods could lead to the reduction of proteins, lipids, and inorganic salts in these two organs. Interestingly, after recovery treatment, the structure and function of the skin and skull could almost recover to the initial states.
Conclusion:In vivo tissue optical clearing can lead to changes in the structure and function of tissue, which was reversible to some extent. This study plays an important role in revealing the underlying mechanisms of tissue optical clearing techniques; moreover, it is conducive to the development and optimization of a novel in vivo tissue optical clearing approaches in future.
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