Kinetics of nutrient enhanced crude oil degradation by Pseudomonas aeruginosa AKS1 and Bacillus sp. AKS2 isolated from Guwahati refinery, India

Chettri, Bobby; Mukherjee, Arghya; Langpoklakpam, James S.; Chattopadhyay, Dhrubajyoti; Singh, Arvind

doi:10.1016/j.envpol.2016.06.008

Cited by 37 publications

(33 citation statements)

References 40 publications

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“…Several studies found that P. aeruginosa had superiority for crude oil biodegradation [15,17,19] . This might be due to their metabolic diversity, abundance in microbial communities, and their resistance to chemical remediation agents [17,19] .…”

Section: Discussionmentioning

confidence: 99%

“…This combination facilitates more exposure of the absorbed oil to the immobilized bacterial consortium. The adherence of oil to the bacterial consortium coated biocarriers is a simple way to boost the substrate uptake speed [15] . The high rate growth of bacterial consortium in carriers especially sponge could be due to the acceleration of oxygen transfer, providing protection and a favorable niche for bacterial consortium to utilize hydrocarbons [27] .…”

Section: Discussionmentioning

confidence: 99%

“…This might be due to their metabolic diversity, abundance in microbial communities, and their resistance to chemical remediation agents [17,19] . Many studies have mentioned that E. sakazakii and K. oxytoca have the ability to degrade the crude oil and can be used for bioremediation purposes [3,15] . In the present study, we isolated B. bronchispetica from Nile water with the ability to degrade crude oil.…”

Section: Discussionmentioning

confidence: 99%

“…An ideal carrier should be nontoxic, nonpolluting, nonbiodegradable, having high cell mass loading capacity, biologically and chemically stable, having a long shelf life, low cost, diffusible by bacteria, easy to handle from media, and easy to regenerate [5,15] . In this study, loofah, palm leaf raffia, and corn-ground qgualh were selected as carriers from agricultural residues, in parallel with the sponge material.…”

Section: Discussionmentioning

confidence: 99%

“…Besides, immobilization is crucial to improve the survival and retention of bacterial cells [13] . Immobilized cell systems provide high biomass and cell reuse, reducing the costly processes of cell recovery and cell recycle [14,15] . In addition, they have high resistance to toxic chemicals, pH and temperature variation, solvents, and heavy metals and they show a decline in maturation time for some products [5,14] .…”

Section: Introductionmentioning

confidence: 99%

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Bioremediation of oil-contaminated water by bacterial consortium immobilized on environment-friendly biocarriers

Samhan

El‐Liethy

Hemdan

et al. 2017

EPX

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Background: Accidental oil spill in the Nile River represents a public health concern in Egypt. Bioremediation of oil-contaminated water is efficiently competitive and economically effective compared with mechanical and chemical methods. Aim:The current study aimed to; i) assess using environment-friendly materials as carriers for immobilizing oil-degrading bacteria and ii) evaluate applying these biocarriers for removal of oil from water in a batch experiment. Materials and Methods: Four bacterial strains Pseudomonas aeruginosa, Cronobacter sakazakii (recently namedEnterobacter sakazakii), Klebsiella oxytoca and Bordetella bronchispetica were immobilized on grinded corn qgualh, Egyptian loofah, palm leaf raffia and sponge. Immobilizing bacterial consortium was carried out by incubating bacteria with carriers at 37°C with shaking at 120 rpm for three days. Biofilm formed on biocarriers was checked using scanning electron microscopy. Biocarriers with immobilized bacterial consortium were used for removal of oil. Experiments were carried out in flasks each containing 100 ml of mineral salt broth fortified with 1 g/l light crude oil. Flasks were incubated at 37°C with shaking at 120 rpm for nine weeks. Total viable bacterial count and oil and grease were determined weekly. Results: Oil and grease measurements showed a significant removal that reached 68.3, 71.5, 77.7 and 81%for grinded corn qgualh, Egyptian loofah, palm leaf raffia and sponge respectively. Flasks inoculated with sponge biocarriers showed rapid oil removal compared with other biocarriers. Gas chromatography-mass spectrometry (GC-MS) showed a percentage removal that reached 87% for compounds C11 to C32 in case of sponge carrier. Conclusion: Immobilizing bacteria on biocarriers showed a good biofilm formation. Immobilized bacterial consortium showed rapid and efficient oil removal in bioremediation processes. Sponge showed dense bacterial colonization and consequently biofilm formation compared with other biocarriers.

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