Kinetics of (Na+,K+)-ATPase of human erythrocyte membranes

Peter, Horst W.; Wolf, Hans

doi:10.1016/0005-2736(72)90072-7

Cited by 20 publications

(5 citation statements)

References 31 publications

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“…This result has been confirmed by Hexum, Samson & Himes (1970) and Skou (1974). On the other hand, Peter & Wolf (1972) reported that increasing K raised the ATP affinity with no effect on the rate at saturating ATP concentrations. Work on the intact red cell has, however, been negative.…”

Section: Ko+ep -E2k-e +Kisupporting

confidence: 53%

The interaction of potassium ions and ATP on the sodium pump of resealed red cell ghosts.

Eisner¹,

Richards²

1981

The Journal of Physiology

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SUMMARY1. Ouabain-sensitive K or Rb influx was measured into ghosts resealed to contain ATP concentrations of 1 ,uM-3 mm and no K.2. Increasing ATP from 1 to 100#sM, at saturating external K, increased K influx about twentyfold while having no effect on the ratio of ouabain-sensitive K influx to ouabain-sensitive ATPase activity.3. Increasing external K decreased the apparent affinity for ATP. Similarly increasing ATP decreased the apparent affinity for external K.4. The K influx can be empirically described as: influx = VmaxK2/(K + Kapp)2. Increasing ATP increased Vmax and (Kapp)2 by the same amount.5. These results are consistent with a consecutive model for the Na pump in which an ATP-dependent reaction follows a K-activated dephosphorylation.

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Section: Ko+ep -E2k-e +Kisupporting

confidence: 53%

The interaction of potassium ions and ATP on the sodium pump of resealed red cell ghosts.

Eisner¹,

Richards²

1981

The Journal of Physiology

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“…As the first product to be released shows linear noncompetitive inhibition and the second product linear competitive inhibition it can be suggested that P, is released before ADP (Hsu et al 1966), This is in agreement with the findings from kinetic studies of a microsomal brain preparation (Hexum et al 1970), Contrariwise most investigations of animal NaK ATPases where labelling technique has been used propose a mechanism where one or two phosphorylated complexes are involved and where ADP is released before P. (Post et al 1965, Sen et al 1969, Lindenmayer et al 1968, Peter and Wolf 1972, Schoner et al, (1966), however, suggested that if the stable acylphosphate exists, this phosphate intermediate would not participate in the Na and K 1,0 1,5 (MgATP) Figure 6, ADP and AMP release at different MgATP concentrations (in mM) in presence or absence of'5 mM /",, In the upper part of tiie figure v is expressed in /imol ADP x (mg protein x min)"' and in the lower part in //mol AMP x (mg protein x min)"'.…”

Section: Ea-epq Eqsupporting

confidence: 69%

ADP, AMP and P_i interactions with a (Na⁺+ K⁺+ Mg²⁺)‐dependent ATPase in sugar beet roots

Lindberg

Björklund

1977

Physiologia Plantarum

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A dithiothreitol treated membrane fraction of sugar beet roots contains (Na++ K++ Mg2+)‐ATPase activity. Product inhibition of the ATPase activity was studied. This led to the following conclusions: 1) ADP is a competitive inhibitor to MgATP in presence or absence of Na+ and/or K+. 2) ADP does not interact with the Na or K site. 3) AMP has little influence on the velocity. 4) Little AMP is released, and the ratio between ADP and Pi release is approximately 1. 5) Pi is a weak noncompetitive inhibitor to MgATP. 6) Pi is probably released before ADP from the Enzyme–MgATP complex.

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“…A linear relation still held when the cellular Nat content was increased to the saturating concentrations at different ATP contents. The linear aspects of these plots are apparently different from that reported for the Na+,K+-ATPase activity as a function of the ATP concentration (Peter and Wolf, 1972). The latter workers showed a Michaelis-Menten-like relation when the ATP concentrations applied were relatively high.…”

Section: Discussionmentioning

confidence: 63%

Rb⁺ influx in response to changes in energy generation: Effect of the regulation of the ATP content of HELa cells

Ikehara

Yamaguchi

Hosokawa

et al. 1984

Journal Cellular Physiology

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The effect of changes in energy metabolism on Rb+ influx was studied in HeLa cells. Irrespective of whether ATP production was controlled by varying the activity of glycolysis or of oxidative metabolism on addition of certain combinations of glucose, carbonylcyanide m-chlorophenylhydrazone, monoiodoacetic acid, and quercetin, Rb+ influx changed as a linear function of the ATP content, which varied in a wide range up to the normal level (15-20 nmol/mg protein or 3-4 mM). The difference between results obtained by these procedures was not significant. As the intracellular Na+ content varied at different ATP contents, the Na+ content was adjusted to similar levels by chilling the cells with varying ATP contents. However, a linear relation was still observed. A similar dependence was also obtained for cytoplasmic ATP, which would be more closely connected with the Na,K-pump than total ATP. The ratio of ouabain-sensitive Rb+ influx to the corresponding part of lactate production was nearly 2 in the presence of 2 mM glucose. From these results it is concluded that (1) active Rb+ influx, which is chiefly maintained by energy generated through glycolysis, can also be supported by oxidative metabolism; (2) Rb+ influx is regulated linearly as a function of the cellular ATP content up to the control level; but does not increase if ATP is raised still further; and (3) 2 Rb+ ions move concomitantly at the expense of one ATP molecule.

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Kinetics of (Na+,K+)-ATPase of human erythrocyte membranes

Cited by 20 publications

References 31 publications

The interaction of potassium ions and ATP on the sodium pump of resealed red cell ghosts.

The interaction of potassium ions and ATP on the sodium pump of resealed red cell ghosts.

ADP, AMP and P_i interactions with a (Na⁺+ K⁺+ Mg²⁺)‐dependent ATPase in sugar beet roots

Rb⁺ influx in response to changes in energy generation: Effect of the regulation of the ATP content of HELa cells

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Kinetics of (Na+,K+)-ATPase of human erythrocyte membranes

Cited by 20 publications

References 31 publications

The interaction of potassium ions and ATP on the sodium pump of resealed red cell ghosts.

The interaction of potassium ions and ATP on the sodium pump of resealed red cell ghosts.

ADP, AMP and Pi interactions with a (Na++ K++ Mg2+)‐dependent ATPase in sugar beet roots

Rb+ influx in response to changes in energy generation: Effect of the regulation of the ATP content of HELa cells

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ADP, AMP and P_i interactions with a (Na⁺+ K⁺+ Mg²⁺)‐dependent ATPase in sugar beet roots

Rb⁺ influx in response to changes in energy generation: Effect of the regulation of the ATP content of HELa cells