Kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7

Kuttiraja, Mathiyazhakan; Dhouha, Ayed; Tyagi, Rajeshwar Dayal

doi:10.1016/j.biortech.2016.09.015

Cited by 32 publications

(26 citation statements)

References 33 publications

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“…Table 9 indicates lipid productivity and lipid yield obtained in this study and that reported in the literature. The lipid yield and productivity observed in purified glycerol was comparable to crude glycerol for Y. lipolytica SKY7 [18]. Lipid productivity recorded in this study was comparable to that of T. oleaginous grown on crude glycerol (with high soap content) [3] and was higher than genetically engineered Y. lipolytica grown on glucose [24], C. curvatus and L. starkeyi grown on crude glycerol [13] [23].…”

Section: Comparison Of Yield Coefficientssupporting

confidence: 59%

“…Irrespective of trace elements, lipid productivity was higher during the initial 16-32-h period because during this Glycerol consumption (g/L) period, membrane lipids develop with biomass growth [18]. While during later stages of fermentation, lipid was accumulated as intracellular lipids in cytosol.…”

Section: Variation Of Biomass Lipid and Ca Productivitiesmentioning

confidence: 99%

“…Fermentations were carried out in stirred tank fermenters (SARTORIUS BIOSTAT, USA and LABFORS 3, INFORS AG, Switzerland) equipped with accessories and programmable logic control (PLC) system. The inoculum size of 6.25% (v/v) was chosen as it is the optimum value reported for Y. lipolytica SKY7 [18]. During the fermentation, DO was maintained between 25 and 40% saturation by adjusting the agitation rate (250-600 rpm) and airflow rate (1-2.5 L/ min).…”

Section: Fermentation Operationmentioning

confidence: 99%

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Optimization of trace elements in purified glycerol for microbial lipid and citric acid production by Yarrowia lipolytica SKY7

Kumar

Yellapu

Tyagi

et al. 2020

Syst Microbiol and Biomanuf

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Purified glycerol obtained after acid treatment of crude glycerol solution was used as the carbon source for lipid and citric acid production using Y. lipolytica SKY7. Although purified glycerol was high in phosphorus, it was important to investigate the impact of fortification of trace elements in the medium on cell growth, lipid and citric acid (CA) production. When all the trace elements (including phosphates and sulfates) required for growth and lipid production were added to the purified glycerol medium, high biomass (51.67 g/L) and lipid concentration (19.47 g/L) were observed at 96 h of fed-batch fermentation with low CA concentration of 5.42 g/L. The purified glycerol medium without additional trace elements gave low biomass (27.67 g/L), lipid concentration (9.35 g/L) at 80 h of fed-batch fermentation, but gave high CA concentration (24.51 g/L). When purified glycerol was provided with only sulfates or all elements except KH 2 PO 4 , low biomass (32.59 g/L and 38.52 g/L) and citric acid concentration (1 g/L and 2.42 g/L) were obtained at 96 h.

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Section: Comparison Of Yield Coefficientssupporting

confidence: 59%

Section: Variation Of Biomass Lipid and Ca Productivitiesmentioning

confidence: 99%

Section: Fermentation Operationmentioning

confidence: 99%

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Optimization of trace elements in purified glycerol for microbial lipid and citric acid production by Yarrowia lipolytica SKY7

Kumar

Yellapu

Tyagi

et al. 2020

Syst Microbiol and Biomanuf

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“…An abundant carbon source can accelerate microbial growth. However, overabundance of carbon sources is not conducive to bacterial reproduction [ 20 ]. Additionally, glycerol was utilized after glucose was exhausted.…”

Section: Resultsmentioning

confidence: 99%

Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis

Chen

et al. 2017

Microb Cell Fact

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BackgroundPoly-gamma-glutamic acid (γ-PGA) is a promising macromolecule with potential as a replacement for chemosynthetic polymers. γ-PGA can be produced by many microorganisms, including Bacillus species. Bacillus licheniformis CGMCC2876 secretes γ-PGA when using glycerol and trisodium citrate as its optimal carbon sources and secretes polysaccharides when using glucose as the sole carbon source. To better understand the metabolic mechanism underlying the secretion of polymeric substances, SWATH was applied to investigate the effect of glucose on the production of polysaccharides and γ-PGA at the proteome level.ResultsThe addition of glucose at 5 or 10 g/L of glucose decreased the γ-PGA concentration by 31.54 or 61.62%, respectively, whereas the polysaccharide concentration increased from 5.2 to 43.47%. Several proteins playing related roles in γ-PGA and polysaccharide synthesis were identified using the SWATH acquisition LC–MS/MS method. CcpA and CcpN co-enhanced glycolysis and suppressed carbon flux into the TCA cycle, consequently slowing glutamic acid synthesis. On the other hand, CcpN cut off the carbon flux from glycerol metabolism and further reduced γ-PGA production. CcpA activated a series of operons (glm and epsA-O) to reallocate the carbon flux to polysaccharide synthesis when glucose was present. The production of γ-PGA was influenced by NrgB, which converted the major nitrogen metabolic flux between NH4 + and glutamate.ConclusionThe mechanism by which B. licheniformis regulates two macromolecules was proposed for the first time in this paper. This genetic information will facilitate the engineering of bacteria for practicable strategies for the fermentation of γ-PGA and polysaccharides for diverse applications.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0642-8) contains supplementary material, which is available to authorized users.

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“…6 It is also easy to culture with a short doubling time. Additionally, Y. lipolytica is well characterized especially for lipid and lipase production, 7,8 and has been shown to be amicable to genetic modication, opening up a wide repertoire of possible applications. 9 Several studies have looked into bulk industrial products such as biodiesel and citric acid as possible products of Y. lipolytica.…”

Section: Introductionmentioning

confidence: 99%

Valorization of papaya fruit waste through low-cost fractionation and microbial conversion of both juice and seed lipids

Han

Park

2018

RSC Adv.

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Kinetics of lipid production at lab scale fermenters by a new isolate of Yarrowia lipolytica SKY7

Cited by 32 publications

References 33 publications

Optimization of trace elements in purified glycerol for microbial lipid and citric acid production by Yarrowia lipolytica SKY7

Optimization of trace elements in purified glycerol for microbial lipid and citric acid production by Yarrowia lipolytica SKY7

Effect of glucose on poly-γ-glutamic acid metabolism in Bacillus licheniformis

Valorization of papaya fruit waste through low-cost fractionation and microbial conversion of both juice and seed lipids

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