Kinetics of inhibition of methane oxidation by nitrate, nitrite, and ammonium in a humisol

Dunfield, Peter F.; Knowles, Roger

doi:10.1128/aem.61.8.3129-3135.1995

Cited by 224 publications

(145 citation statements)

References 33 publications

(77 reference statements)

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“…Kinetic calculations suggest that Methylocystis could be responsible for the observed CH 4 oxidation activity. The K mðappÞ values measured in the surface 20 cm of the meadow soil (137^300 nM) were similar to those measured in a periodically £ooded humisol in Canada (100^280 nM) [41] and in Methylocystis strain LR1 isolated from this humisol (as low as 60^250 nM under certain growth conditions) [31,42]. Soils which are net CH 4 sources, such as peats and rice ¢elds, generally display much lower a⁄nity for CH 4 (i.e.…”

Section: Discussionsupporting

confidence: 68%

Activity and community structure of methane-oxidising bacteria in a wet meadow soil

Horz

2002

FEMS Microbiology Ecology

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The structure and activity of the methane-oxidising microbial community in a wet meadow soil in Germany were investigated using biogeochemical, cultivation, and molecular fingerprinting techniques. Both methane from the atmosphere and methane produced in anaerobic subsurface soil were oxidised. The specific affinity (first-order rate constant) for methane consumption was highest in the top 20 cm of soil and the apparent half-saturation constant was 137^300 nM CH 4 , a value intermediate to measured values in wetland soils versus well-aerated upland soils. Most-probable-number (MPN) counting of methane-oxidising bacteria followed by isolation and characterisation of strains from the highest positive dilution steps suggested that the most abundant member of the methane-oxidising community was a Methylocystis strain (10 5^1 0 7 cells g 31 d.w. soil). Calculations based on kinetic data suggested that this cell density was sufficient to account for the observed methane oxidation activity in the soil. DNA extraction directly from the same soil samples, followed by PCR amplification and comparative sequence analyses of the pmoA gene, also detected Methylocystis. However, molecular community fingerprinting analyses revealed a more diverse and dynamic picture of the methane-oxidising community. Retrieved pmoA sequences included, besides those closely related to Methylocystis spp., others related to the genera Methylomicrobium and Methylocapsa, and there were differences across samples which were not evident in MPN analyses. ß

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Section: Discussionsupporting

confidence: 68%

Activity and community structure of methane-oxidising bacteria in a wet meadow soil

Horz

2002

FEMS Microbiology Ecology

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“…These findings do not concur with a study by (Gauci & Chapman, 2006) that was unable to detect any effect of sulfur deposition on CH 4 oxidation, despite a decrease in CH 4 emission. Inhibition of CH 4 oxidation from different nitrogen compounds, predominantly NH 4 1 , is well known from both upland (Dunfield & Knowles, 1995;Saari et al, 1997;Gulledge & Schimel, 1998;Wang & Ineson, 2003) and wetland soils (Crill et al, 1994;Kravchenko, 2002). However, the mire in this study is severely nitrogen limited.…”

Section: Effects On Potential Ch 4 Oxidationmentioning

confidence: 99%

Production and oxidation of methane in a boreal mire after a decade of increased temperature and nitrogen and sulfur deposition

Eriksson

Öquist

Nilsson

2010

Global Change Biology

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Natural wetlands are the single largest source of atmospheric methane (CH 4 ). Both a changed climate and deposition of anthropogenic nitrogen and sulfur can alter the production and oxidation of CH 4 respectively and thereby also CH 4 exchange. We used a long-term (12 years) factorial field experiment in a boreal oligotrophic mire to evaluate the effects of greenhouse cover and addition of ammonium nitrate and sodium sulfate on the production and oxidation of CH 4 by applying laboratory incubations of samples from five depths in the mire. The rates of CH 4 production were measured without amendments and after the addition of either glucose or sulfate. Twelve years of increased nitrogen deposition has changed the mire from a Sphagnum-dominated plant community to one dominated by sedges and dwarf shrubs. The deposition of nitrogen to the field plots caused increased production of CH 4 in incubations without amendments (34%), and also after amendments with glucose (40%) or sulfate (42%). This indicates increased substrate availability (without amendments) but also a greater abundance of methanogens (glucose amendment). The greenhouse cover caused a decrease in CH 4 production in incubations without amendments (34%), after glucose amendment (20%) and after sulfate amendment (31%). These responses indicate decreased substrate availability (without amendment) accompanied by the reduced abundance of methanogens (glucose amendment). The field application of sulfur had no effect on CH 4 production at the depth where maximal CH 4 production occurred. Closer to the mire surface, however, the rate of CH 4 production was significantly reduced by 32-45%. These results suggest that the deposition of sulfate has altered the vertical distribution of methanogens and sulfate-reducing bacteria. The oxidation of CH 4 was not significantly affected by any of the long-term field treatments.

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“…Nitrogen fertilizers stimulate crop growth and provide more carbon substrates (via organic root exudates and sloughed-off cells) to Methanogens for CH 4 production (Aulakh et al, 2001;van der Gon et al, 2002;Inubushi et al, 2003). In addition to crop growth, N fertilizers alter the activities of Methanotrophs in the soils (Schimel, 2000;Bodelier & Laanbroek, 2004).…”

Section: Introductionmentioning

confidence: 99%

Do nitrogen fertilizers stimulate or inhibit methane emissions from rice fields?

Banger

Tian

Lü

2012

Global Change Biology

239

119

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In rice cultivation, there are controversial reports on net impacts of nitrogen (N) fertilizers on methane (CH ) emissions. Nitrogen fertilizers increase crop growth as well as alter CH producing (Methanogens) and consuming (Methanotrophs) microbes, and thereby produce complex effects on CH emissions. Objectives of this study were to determine net impact of N fertilizers on CH emissions and to identify their underlying mechanisms in the rice soils. Database was obtained from 33 published papers that contained CH emissions observations from N fertilizer (28-406 kg N ha ) treatment and its control. Results have indicated that N fertilizers increased CH emissions in 98 of 155 data pairs in rice soils. Response of CH emissions per kg N fertilizer was significantly (P < 0.05) greater at < 140 kg N ha than > 140 kg N ha indicating that substrate switch from CH to ammonia by Methanotrophs may not be a dominant mechanism for increased CH emissions. On the contrary, decreased CH emission in intermittent drainage by N fertilizers has suggested the stimulation of Methanotrophs in rice soils. Effects of N fertilizer stimulated Methanotrophs in reducing CH emissions were modified by the continuous flood irrigation due to limitation of oxygen to Methanotrophs. Greater response of CH emissions per kg N fertilizer in urea than ammonia sulfate probably indicated the interference of sulfate in the CH production process. Overall, response of CH emissions to N fertilizers was correlated with N-induced crop yield (r = +0.39; P< 0.01), probably due to increased carbon substrates for Methanogens. Using CH emission observations, this meta-analysis has identified dominant microbial processes that control net effects of N fertilizers on CH emissions in rice soils. Finally, we have provided a conceptual model that included microbial processes and controlling factors to predict effects of N fertilizers on CH emissions in rice soils.

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Kinetics of inhibition of methane oxidation by nitrate, nitrite, and ammonium in a humisol

Cited by 224 publications

References 33 publications

Activity and community structure of methane-oxidising bacteria in a wet meadow soil

Activity and community structure of methane-oxidising bacteria in a wet meadow soil

Production and oxidation of methane in a boreal mire after a decade of increased temperature and nitrogen and sulfur deposition

Do nitrogen fertilizers stimulate or inhibit methane emissions from rice fields?

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