Kinetics of induced wound repair at 0°C in the Antarctic fish (Cabeçuda) Notothenia coriiceps

Silva, José Roberto Machado Cunha da; Sinhorini, I L; Jensch‐Junior, Bernard Ernesto; Porto-Neto, Laércio R.; Hernadez-Blazquez, Francisco Javier; Vellutini, Bruno Cossermelli; Pressinotti, Leandro Nogueira; Costa-Pinto, Frederico Azevedo da; Cooper, Edwin L.; Borges, João Carlos Shimada

doi:10.1007/s00300-004-0611-7

Cited by 12 publications

(14 citation statements)

References 17 publications

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“…However, although membrane specializations may help fortify the skin of G. acuticeps against the propagation of ice (Eastman and Hikida, 1991), the protection offered by the skin likely depends predominantly on its physical condition, because damaged skin probably offers little resistance to the propagation of ice. Even though larval skin is delicate and only a few cells thick at the time of hatching (Rombough, 1988), it may be less likely to suffer from lesions and other damage than that of the adult stages, which has a much larger surface area and may be slow at healing at the cold environmental temperatures (da Silva et al, 2004). This is consistent with the organismal FPs of adult fish in the presence of external ice being only slightly below the FPs of their body fluids, while excised skin sections, which have been carefully selected for their integrity (Valerio et al, 1992b), and the skin of larval G. acuticeps and P. antarcticum (Table·2), offer more protection.…”

Section: Environmental Ice and Freezing Resistancementioning

confidence: 99%

Freezing resistance of antifreeze-deficient larval Antarctic fish

Cziko

Evans

Cheng

et al. 2006

Journal of Experimental Biology

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Antarctic notothenioids, along with many other polar marine fishes, have evolved biological antifreeze proteins (AFPs) to survive in their icy environments. The larvae of Antarctic notothenioid fish hatch into the same frigid environment inhabited by the adults, suggesting that they must also be protected by sufficient AFPs, but this has never been verified. We have determined the contribution of AFPs to the freezing resistance of the larvae of three species: Gymnodraco acuticeps, Pagothenia borchgrevinki and Pleuragramma antarcticum. Of the three, only P. borchgrevinki larvae are protected by high, adult levels of AFPs. Hatchling G. acuticeps and P. antarcticum have drastically inadequate AFP concentrations to avoid freezing at the ambient seawater temperature (-1.91°C). We raised G. acuticeps larvae and measured the AFP levels in their blood for ~5 months post hatching. Larval serum freezing point was -1.34±0.04°C at the time of hatch; it began to decrease only after 30 days post hatch (d.p.h.), and finally reached the adult value (-2.61±0.03°C) by 147·d.p.h. Additionally, AFP concentrations in their intestinal fluids were very low at hatching, and did not increase with age throughout a sampling period of 84·d.p.h.Surviving in a freezing environment without adequate AFP protection suggests that other mechanisms of larval freezing resistance exist. Accordingly, we found that G. acuticeps hatchlings survived to -3.6±0.1°C while in contact with external ice, but only survived to -1.5±0.0°C when ice was artificially introduced into their tissues. P. antarcticum larvae were similarly resistant to organismal freezing. The gills of all three species were found to be underdeveloped at the time of hatch, minimizing the risk of ice introduction through these delicate structures. Thus, an intact integument, underdeveloped gill structures and other physical barriers to ice propagation may contribute significantly to the freezing resistance and survival of these larval fishes in the icy conditions of the Southern Ocean.

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Section: Environmental Ice and Freezing Resistancementioning

confidence: 99%

Freezing resistance of antifreeze-deficient larval Antarctic fish

Cziko

Evans

Cheng

et al. 2006

Journal of Experimental Biology

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“…A fagocitose está diretamente envolvida nos processos decorrentes da capacidade que os animais possuem de manter a integridade do tegumento após uma lesão. A maneira pela qual estes animais realizam os processos de cicatrização e regeneração para manterem sua integridade entre o ambiente interno (animal) e o externo (meio) também foi objeto de nossos estudos , Silva et al 2004.…”

Section: Cicatrizaçãounclassified

“…A importância da resposta inflamatória reside em muitos aspectos, tais como a provisão de células importantes para a remoção de debris celulares, a preparação para responder a uma possível infecção bacteriana, a secreção de substâncias necessárias para estimular a migração de fibroblastos (necessários para síntese de matriz extracelular) e proliferação de outras células (como as epiteliais, a partir das suas precursoras na camada basal); enfim, de quase todas as fases subseqüentes da reparação das lesões (Silva et al 2004). A restituição tecidual sempre visa o fechamento da área lesada, selando-a, se necessário, com uma cicatriz, e retomando sempre que possível, sua função original (Silva 1998a).…”

Section: Cicatrizaçãounclassified

“…Foi realizado um estudo da cicatrização em N. coriiceps pela primeira vez (Silva et al 2004(Silva et al , 2005, sendo o primeiro estudo de cicatrização sob temperaturas polares em ectotérmicos.…”

Section: Cicatrizaçãounclassified

“…Este estudo mostra pela primeira vez a capacidade germicida dos macrófagos de peixes antárticos, apesar da baixa temperatura em que vivem (0 o C). (Silva et al 2004, Silva et al 2005 O objetivo do presente projeto foi caracterizar histologicamente a cicatrização cutânea do peixe antártico Notothenia coriiceps, mantido a 0 a 2 o C. Após a anestesia foram realizadas incisões padronizadas para remoção de uma área de 4 cm 2 na região dorso lateral anterior. Verificamos um deslizamento do epitélio a partir das bordas da ferida e após 23 dias a lesão se fecha por completo, havendo uma concomitante retração dos bordos da ferida após 10 dias e continuando até o prazo máximo estudado.…”

Section: Fagocitose E Capacidade Germicida Dos Macrófagos (M∅) In Vitunclassified

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Resposta Imune Inespecífica De Animais Ectotérmicos Antárticos Sob Temperaturas Polares

et al. 2007

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RESUMOEsta revisão tem por objetivo apresentar os dados sobre a imunidade inespecífica de metazoários pecilotérmicos antárticos obtidos por nosso grupo de pesquisa com animais antárticos. Trataremos em particular dos processos de fagocitose, inflamação e cicatrização em peixes antárticos popularmente chamados "cabeçudas" (Notothenia coriiceps = N. neglecta), em estrelas-do-mar antárticas (Odontaster validus) e em ouriços-do-mar antárticos (Sterechinus neumayeri). Palavras-chaves: Fagocitose, inflamação, cicatrização, resposta imune inespecífica, Antártica. ABSTRACT UNSPECIFIC IMMUNE RESPONSE OF ANTARCTIC ECTOTERMIC ANIMALS UNDER POLAR TEMPERATURES.This review presents data on nonspecific immune system of Antarctic metazoans poekiloterms. The phagocytosis, inflammation and wound repair were analysed in Antarctic animals, the big head fish (Notothenia coriiceps = N. neglecta), Antarctic starfish (Odontaster validus) and in Antarctic sea urchin (Sterechinus neumayeri).

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Healing of cutaneous wounds in a freshwater teleost, Labeo rohita: Scanning electron microscopical investigation

Kumari¹,

Srivastava²,

Nigam³

et al. 2012

Microscopy Res & Technique

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In this study, healing of cutaneous wounds in Labeo rohita using scanning electron microscope is reported. Wound area could be divided into three regions. Immediately after infliction of wound, edges retract exposing underlying tissues in wound gap (Region I). Simultaneously, at region close to wound edge (Region II), mucous goblet cell openings are observed with copious mucous secretions. Within 1 h, Region I gets covered by mucous secretions, and epidermis at edges starts migrating. Opposing fronts gradually advance and by 4-6 h come in contact to epithelialize wound gap. Zone of contact of fronts is demarcated by epidermal ridge, which is relatively prominent at 8 h. It gradually diminishes and is not distinguished at 24 h and afterward. At 1-4 h, microridges on epithelial cell surfaces appear irregularly arranged, widely spaced, short with abrupt ends at Region I; relatively extensive at Region II; and similar to those in controls at region surrounding Region II (Region III). At 12 h and afterward, microridges appear similar to those in controls at Regions I and II. At 1-2 h, isolated swollen epithelial cells, often in process of detachment and exfoliation at surface, are observed at Regions I and II. Such cells are infrequent at 8 h and afterward. Region I covered by migrated epidermis appears trough like at 4 h to 2 days, level of which gradually rises and at Day 4, surface of epidermis appears at a level similar to that at Regions II and III. Changes have been associated with the imbalance of osmotic homeostasis due to disruption of barrier between internal and external environment of skin.

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Kinetics of induced wound repair at 0°C in the Antarctic fish (Cabeçuda) Notothenia coriiceps

Cited by 12 publications

References 17 publications

Freezing resistance of antifreeze-deficient larval Antarctic fish

Freezing resistance of antifreeze-deficient larval Antarctic fish

Resposta Imune Inespecífica De Animais Ectotérmicos Antárticos Sob Temperaturas Polares

Healing of cutaneous wounds in a freshwater teleost, Labeo rohita: Scanning electron microscopical investigation

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