Kinetics of IFN-gamma, TNF-alpha, IL-10 and IL-4 production by mononuclear cells stimulated with gp43 peptides, in patients cured of paracoccidioidomycosis

Sadahiro, Aya; Diogo, Constância Lima; Oshiro, Telma Miyuki; Shikanai‐Yasuda, Maria Aparecida

doi:10.1590/s0037-86822007000200003

Cited by 11 publications

(7 citation statements)

References 23 publications

(13 reference statements)

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“…In patients with PCM, higher levels of IL‐10 have been associated with poorly organized granulomas in an adjuvant effect with IL‐5 [27]. However, this cytokine was also shown to control the number of apoptotic cells, with no difference in the levels between patients with active and treated PCM when assayed on PBMCs [4, 6, 28]. Conversely, monocytes from patients with active PCM endogenously produce high amounts of IL‐10 [29].…”

Section: Discussionmentioning

confidence: 99%

“…Conversely, the Th2 cytokine profile has been associated with acute PCM and with patients with disseminated disease, resulting in an imbalance in the cytokine profile and in transitory immunodepression in response to P. brasiliensis antigens and its immunodominant component, the 43‐kDa glycoprotein, with recovery of immune responses occurring after clinical cure. In this context, the chronic form of PCM could represent a mixed Th1 and Th2 immune response [5–7].…”

Section: Introductionmentioning

confidence: 99%

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Characterization of Monocyte‐Derived Dendritic Cells from Patients with Active and Treated Paracoccidioidomycosis

Sato

Oshiro²,

Diogo

et al. 2011

Scand J Immunol

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Cellular immune responses are a significant defence mechanism in human paracoccidioidomycosis (PCM), an endemic mycosis in Latin America; however, little is known about the role of dendritic cells (DCs) in human PCM. We investigated monocyte‐derived DCs from patients with treated (TP) and active PCM (AP) compared with healthy non‐PCM donors (CO). DCs from the TP group showed higher expression of HLA‐DR, CD86 and DC‐SIGN compared with CO, whereas AP showed similar expression to CO. Production of IL‐10 was downregulated by TNF‐α in all groups and lower levels were observed in untreated DCs from AP compared with CO. Conversely, IL‐12p40 was significantly upregulated in the DCs of the TP group. TNF‐α‐activated DCs from the CO group produced significantly lower levels of IL‐12p40 when differentiated from magnetic‐sorted monocytes (MACS) compared with adhered monocyte‐derived DCs. This comparison in the TP group revealed similar levels of IL‐12p40, suggesting a T cell–independent increase in the production of IL‐12p40. Higher expression of surface molecules with increased IL‐12p40 may indicate a better activation of DCs after the treatment of PCM. Our findings suggest that DCs may be crucial in the protective response to Paracoccidioides brasiliensis and that in vitro‐generated DCs might be useful in enhancing antifungal immunity, especially during active PCM.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization of Monocyte‐Derived Dendritic Cells from Patients with Active and Treated Paracoccidioidomycosis

Sato

Oshiro²,

Diogo

et al. 2011

Scand J Immunol

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“…In this regard, a recent report described the kinetics of the secretion of some cytokines in response to mixtures of gp43 peptides by cells from patients cured of PCM. There was considerable variability both in the patients' secretion levels and in secretion kinetics among the cytokines [74]. Peptide pools combining these peptides may represent a better alternative [73], but this awaits further studies.…”

Section: Modifying the Immune Responses?mentioning

confidence: 99%

An overview of the immunopathology of human paracoccidioidomycosis

2008

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We review here the advances in the understanding of the immunopathology of human paracoccidioidomycosis (PCM). Its investigation must take in account the intriguing natural history of the mycosis and its agent, providing clues to the mechanisms that lead to development of disease (unbalanced host-parasite relationship?) or to the clinically silent, chronic carrier state (balanced hostparasite relationship?), in exposed people living in endemic areas. Although the literature on this subject has progressed notably, the overall picture of what are the mechanisms of susceptibility or resistance continues to be fragmentary. Major advances were seen in the description of both the cytokines/chemokines associated to the different outcomes of the hostparasite interaction, and the fungus-monocyte/macrophage interaction, and cytokines released thereof by these cells. However, relatively few studies have attempted to modify, even in vitro, the patients' unbalanced immune reactivity. Consequently, the benefits of this improved knowledge did not yet reach clinical practice. Fortunately, the previous notion of the immune system as having two nearly independent arms, the innate and adaptive immunities, leaving a large gap between them, is now being overcome.Immunologists are now trying to dissect the connections between these two arms. This will certainly lead to more productive results. Current investigations should address the innate immunity events that trigger the IL-12/IFN-c axis and confer protection against PCM in those individuals living in endemic areas, who have been infected, but did not develop the mycosis.

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“…There is little modulation of Th-1 type cytokines during the immune response to acute phase disease and Th-2 type cytokines are predominantly expressed during this phase, leading to the activation and differentiation of B lymphocytes into plasma cells and an increase in the levels of immunoglobulins, IL-4, IL-5 and IL-10. However, during chronic and disseminated PCM, there are high levels of TNF-α, IL-1 and IL-6 and a normal level of IL-4 (Sadahiro et al 2007, Bernard 2008, Ramos & Saraiva 2008.…”

mentioning

confidence: 99%

Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet

Sargi

Dalalio

Visentainer

et al. 2012

Mem. Inst. Oswaldo Cruz

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Kinetics of IFN-gamma, TNF-alpha, IL-10 and IL-4 production by mononuclear cells stimulated with gp43 peptides, in patients cured of paracoccidioidomycosis

Abstract: We analyzed the kinetics of cytokine production by mononuclear cells from 17

Cited by 11 publications

References 23 publications

Characterization of Monocyte‐Derived Dendritic Cells from Patients with Active and Treated Paracoccidioidomycosis

Characterization of Monocyte‐Derived Dendritic Cells from Patients with Active and Treated Paracoccidioidomycosis

An overview of the immunopathology of human paracoccidioidomycosis

Production of TNF-α, nitric oxide and hydrogen peroxide by macrophages from mice with paracoccidioidomycosis that were fed a linseed oil-enriched diet

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