Kinetics of Human and Bovine Serum Albumin Adsorption at Silica–Titania Surfaces

Kurrat, R.; Prenosil, J. E.; Ramsden, Jeremy J.

doi:10.1006/jcis.1996.4528

Cited by 171 publications

(179 citation statements)

References 6 publications

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“…5). Adsorption on the uncoated Si 0.6 Ti 0.4 O 2 chip resulted in an essentially stable monolayer as observed previously [59]. Adsorption was increased by 92 ± 16% on the nanostructured titanate coating, while still resulting in an essentially stable monolayer (as inferred from the saturation of the adsorption with minor desorption after removal of the protein from the bulk solution).…”

Section: Protein Adsorption Experiments On the Tnt-coated And Uncoatesupporting

confidence: 77%

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Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Nádor

Orgován

Fried

et al. 2014

Colloids and Surfaces B: Biointerfaces

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Please cite this article in press as: J. Nador, et al., Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips, Colloids Surf. a b s t r a c tA new type of titanate nanotube (TNT) coating is investigated for exploitation in biosensor applications. The TNT layers were prepared from stable but additive-free sols without applying any binding compounds. The simple, fast spin-coating process was carried out at room temperature, and resulted in well-formed films around 10 nm thick. The films are highly transparent as expected from their nanostructure and may, therefore, be useful as coatings for surface-sensitive optical biosensors to enhance the specific surface area. In addition, these novel coatings could be applied to medical implant surfaces to control cellular adhesion. Their morphology and structure was characterized by spectroscopic ellipsometry (SE) and atomic force microscopy (AFM), and their chemical state by X-ray photoelectron spectroscopy (XPS). For quantitative surface adhesion studies, the films were prepared on optical waveguides. The coated waveguides were shown to still guide light; thus, their sensing capability remains. Protein adsorption and cell adhesion studies on the titanate nanotube films and on smooth control surfaces revealed that the nanostructured titanate enhanced the adsorption of albumin; furthermore, the coatings considerably enhanced the adhesion of living mammalian cells (human embryonic kidney and preosteoblast).

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Section: Protein Adsorption Experiments On the Tnt-coated And Uncoatesupporting

confidence: 77%

“…orders of magnitude greater than the size of the protein molecule and there is no hydromechanical force on the protein [59,60].…”

Section: Protein Adsorption Experiments On the Tnt-coated And Uncoatementioning

confidence: 99%

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Nádor

Orgován

Fried

et al. 2014

Colloids and Surfaces B: Biointerfaces

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show abstract

“…Therefore, BSA was substituted for HSA in our study. 12,13 Despite excellent biological properties in using albumin nanoparticles as drug delivery vehicles, further investigations on optimizing their ability to selectively target cancer cells and releasing chemotherapeutical agents in a controlled manner are much needed. To solve the problem of site-specific targeting, some researchers have attempted to enhance tissue specificity of albumin-based drug carriers by coupling them with targeting agents, such as follic acid 14 and transferrin.…”

Section: Introductionmentioning

confidence: 99%

RGD-conjugated albumin nanoparticles as a novel delivery vehicle in pancreatic cancer therapy

Zhang

et al. 2012

Cancer Biology & Therapy

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These authors contributed equally to this work. Keywords: nanoparticles, albumin, RGD, FITC, delivery, pancreatic cancer, therapyIntegrin avb3 receptor is expressed on several types of cancer cells, including pancreatic cancer cells, and plays an important role in tumor growth and metastasis. The ability to target the integrin avb3 receptor on cancer cells increases the efficacy of targeted therapy and reduces the side effects. The aim of this study is to develop a novel arginine-glycineaspartic acid (RGD) peptide-conjugated albumin nanoparticle to enhance the intracellular uptake of anticancer drug into the pancreatic cancer cells through receptor-mediated endocytosis. In cellular uptake studies, the fluorescent signal of RGD-conjugated BSANPs in BxPC3 cells was higher than that of BSANPs without RGD conjugation as determined by fluorescence spectrophotometer. We also found that BSANPs bound to BxPC3 cells in a time-and concentration-dependent manner. The uptake of RGD-conjugated BSANPs by pancreatic cancer cells was inhibited by an excess amount of free RGD peptide, indicating that the binding and/or uptake were mediated by the avb3 receptor. Furthermore, the nanoparticles were found to be located close to the nuclei by using laser scanning confocal microscopy. Besides, no significant in vitro cytotoxicity was observed as measured with MTT assay. Both in vitro and in vivo antitumor efficacy was improved by targeting gemcitabine-loaded nanoparticles to BxPC-3 cells using RGD peptides. Therefore, the RGD-conjugated BSANPs hold great potential as an effective drug delivery system to deliver therapeutic agents to pancreatic cancer.

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“…2 This attribute has been demonstrated in the context of the adsorption of proteins at solid/liquid interfaces both theoretically 3 and experimentally. [4][5][6] Compact globular proteins ͑such as the abundant soluble proteins of the blood, e.g., albumin and transferrin͒ have been extensively investigated for decades, and many features of their behavior are understood; highly elongated fibrous proteins are known to follow more complicated multistructural trajectories ͑e.g., Refs. 7 and 8͒, and not all details of their adsorption and desorption processes have as yet been elucidated; even less is presently known about an even more complex class of objects, giant glycoproteins such as mucin, which have only recently become available in sufficiently high purity to permit truly quantitative experimentation with them.…”

mentioning

confidence: 99%

Structural hysteresis and hierarchy in adsorbed glycoproteins

Horváth

Yakubov

Ramsden

2008

The Journal of Chemical Physics

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The adsorption and desorption of the giant heavily glycosylated protein mucin from solutions of different bulk concentrations have been followed at the nanometer scale using high resolution molecular microscopy based on optical waveguide lightmode spectroscopy. Modeling the layer as a uniaxial thin film allowed the in situ determination of adsorbed mass, mean layer thickness, and structural anisotropy. These parameters manifest highly significant adsorption-desorption hysteresis, indicating at least two dominant glycoprotein conformational types (i.e., molecular states, structurally and kinetically distinguishable). One of them is proposed to be a conformationally extended state that engenders uniaxial symmetry and dominates layers generated from low bulk concentrations. The revealed structure and the mechanism by which it is formed are postulated to be a general feature of the self-assembly of large glycoproteins. We expect that, inter alia, this knowledge will be relevant for understanding the extraordinary effectiveness of mucin thin films as boundary lubricants.

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Kinetics of Human and Bovine Serum Albumin Adsorption at Silica–Titania Surfaces

Cited by 171 publications

References 6 publications

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

Enhanced protein adsorption and cellular adhesion using transparent titanate nanotube thin films made by a simple and inexpensive room temperature process: Application to optical biochips

RGD-conjugated albumin nanoparticles as a novel delivery vehicle in pancreatic cancer therapy

Structural hysteresis and hierarchy in adsorbed glycoproteins

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