Kinetics of glucocorticoid exposure in developing zebrafish: A tracer study

Steenbergen, Peter J.; Bardine, Nabila; Sharif, Faiza

doi:10.1016/j.chemosphere.2017.05.059

Cited by 9 publications

(10 citation statements)

References 69 publications

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“…The overall weak effects caused by dexamethasone, however, could also be due to reduced bioavailability of the compound. It has been reported that embryonic concentrations reached only 20% of the exposure concentrations indicating a potential slow uptake and internal concentration not in equilibrium (Steenbergen et al , 2017). Uptake of the chemicals by zebrafish embryos was not analyzed in our study, as the focus was on feature detection and quantification of developmental toxicity.…”

Section: Discussionmentioning

confidence: 99%

Automated Morphological Feature Assessment for Zebrafish Embryo Developmental Toxicity Screens

Teixidó

Kießling

Krupp

et al. 2018

Toxicological Sciences

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Detection of developmental phenotypes in zebrafish embryos typically involves a visual assessment and scoring of morphological features by an individual researcher. Subjective scoring could impact results and be of particular concern when phenotypic effect patterns are also used as a diagnostic tool to classify compounds. Here we introduce a quantitative morphometric approach based on image analysis of zebrafish embryos. A software called FishInspector was developed to detect morphological features from images collected using an automated system to position zebrafish embryos. The analysis was verified and compared with visual assessments of 3 participating laboratories using 3 known developmental toxicants (methotrexate, dexamethasone, and topiramate) and 2 negative compounds (loratadine and glibenclamide). The quantitative approach exhibited higher sensitivity and made it possible to compare patterns of effects with the potential to establish a grouping and classification of developmental toxicants. Our approach improves the robustness of phenotype scoring and reliability of assay performance and, hence, is anticipated to improve the predictivity of developmental toxicity screening using the zebrafish embryo.

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Section: Discussionmentioning

confidence: 99%

Automated Morphological Feature Assessment for Zebrafish Embryo Developmental Toxicity Screens

Teixidó

Kießling

Krupp

et al. 2018

Toxicological Sciences

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“…We present evidence that PSNPs induce changes in both glucose and cortisol levels, as well as in gluconeogenesis activity in zebrafish larvae. Given the direct involvement of cortisol in increased activity 30 , we have subsequently examined behavioural changes by measuring distance moved during alternating light–dark cycles as a common behavioural trigger in fish using wild-type, hyperglycemic, and gr mutant larvae exposed to PSNPs. We confirmed that glucose homoeostasis, as well as the Gr, are likely mediating the observed changes in behaviour.…”

Section: Introductionmentioning

confidence: 99%

Polystyrene nanoplastics disrupt glucose metabolism and cortisol levels with a possible link to behavioural changes in larval zebrafish

et al. 2019

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Plastic nanoparticles originating from weathering plastic waste are emerging contaminants in aquatic environments, with unknown modes of action in aquatic organisms. Recent studies suggest that internalised nanoplastics may disrupt processes related to energy metabolism. Such disruption can be crucial for organisms during development and may ultimately lead to changes in behaviour. Here, we investigated the link between polystyrene nanoplastic (PSNP)-induced signalling events and behavioural changes. Larval zebrafish exhibited PSNP accumulation in the pancreas, which coincided with a decreased glucose level. By using hyperglycemic and glucocorticoid receptor (Gr) mutant larvae, we demonstrate that the PSNP-induced disruption in glucose homoeostasis coincided with increased cortisol secretion and hyperactivity in challenge phases. Our work sheds new light on a potential mechanism underlying nanoplastics toxicity in fish, suggesting that the adverse effect of PSNPs are at least in part mediated by Gr activation in response to disrupted glucose homeostasis, ultimately leading to aberrant locomotor activity.

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“…Eggs were exposed to these solutions for 6 hrs. It has been shown that both cortisol and dexamethasone diffuse inside the eggs in this period (37). In addition, we have already shown in earlier studies that this procedure is effective in eliciting changes in physiology and behavior at 3-5 dpf (10,36).…”

Section: Subjects Spawning and Carementioning

confidence: 55%

Early Life Glucocorticoid Exposure Modulates Immune Function in Zebrafish (Danio rerio) Larvae

et al. 2020

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In this study we have assessed the effects of increased cortisol levels during early embryonic development on immune function in zebrafish (Danio rerio) larvae. Fertilized eggs were exposed to either a cortisol-containing, a dexamethasone-containing (to stimulate the glucocorticoid receptor selectively) or a control medium for 6 h post-fertilization (0-6 hpf). First, we measured baseline expression of a number of immune-related genes (socs3a, mpeg1.1, mpeg1.2, and irg1l) 5 days post-fertilization (dpf) in larvae of the AB and TL strain to assess the effectiveness of our exposure procedure and potential strain differences. Cortisol and dexamethasone strongly up-regulated baseline expression of these genes independent of strain. The next series of experiments were therefore carried out in larvae of the AB strain only. We measured neutrophil/macrophage recruitment following tail fin amputation (performed at 3 dpf) and phenotypical changes as well as survival following LPS-induced sepsis (150 µg/ml; 4-5 dpf). Dexamethasone, but not cortisol, exposure at 0-6 hpf enhanced neutrophil recruitment 4 h post tail fin amputation. Cortisol and dexamethasone exposure at 0-6 hpf led to a milder phenotype (e.g., less tail fin damage) and enhanced survival following LPS challenge compared to control exposure. Gene-expression analysis showed accompanying differences in transcript abundance of tlr4bb, cxcr4a, myd88, il1β, and il10. These data show that early-life exposure to cortisol, which may be considered to be a model or proxy of maternal stress, induces an adaptive response to immune challenges, which seems mediated via the glucocorticoid receptor.

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Kinetics of glucocorticoid exposure in developing zebrafish: A tracer study

Cited by 9 publications

References 69 publications

Automated Morphological Feature Assessment for Zebrafish Embryo Developmental Toxicity Screens

Automated Morphological Feature Assessment for Zebrafish Embryo Developmental Toxicity Screens

Polystyrene nanoplastics disrupt glucose metabolism and cortisol levels with a possible link to behavioural changes in larval zebrafish

Early Life Glucocorticoid Exposure Modulates Immune Function in Zebrafish (Danio rerio) Larvae

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