Kinetics of gene expression of alkaline phosphatase during healing of alveolar bone in rats

Abstract

Immunohistochemical studies and molecular biology have enabled us to identify numerous proteins that are involved in the metabolism of bone, and their encoding genes. Among these is alkaline phosphatase (ALP), an enzyme that is responsible for the initiation of mineralisation of the extracellular matrix during alveolar bone repair. To evaluate the gene expression of ALP during this process, we studied nine healthy adult male rats, which had their maxillary central incisors extracted from the right side and were randomly divided into three groups. During three experimental periods, 7 days, 14 days, and 28 days, the alveoli were curetted, the rats killed, and samples analysed by real-time reverse transcription polymerase chain reaction (qRT-PCR). The RNAm that encodes the gene for the synthesis of ALP was expressed during the three periods analysed, but its concentration was significantly increased at 14 and 28 days compared with at 7 days. There was no significant difference between 14 and 28 days (p=0.0005). We conclude that genes related to ALP are expressed throughout the healing process and more intensively during the later periods (14 and 28 days), which coincides with the increased formation of mineralised bone.

The impact of Resolvin E1 on bone regeneration in critical‐sized calvarial defects of rat model—A gene expression and micro‐CT analysis

“…44 However, the mechanism for such ALP expression is unexplored. 45 In the current study, an increase in OCN expression in RvE1 + bovine xenograft, RvE1 and positive control groups represent the osteogenesis activity in these groups. A biomarker for bone formation, osteocalcin (OCN) expression reflects osteoblastic activity and bone remodelling.…”

The Impacts of Ramadan Intermittent Fasting on Saliva Flow-Rate and Metabolic Data: A Systematic Review

“…Alkaline phosphatase and aminotransferase are usually measured together to investigate the hepatic, cardiovascular, and renal functions ( 43 ). ALP is a protein produced by various cell types (e.g., polymorphonuclear leukocytes, osteoblasts, macrophages, and fibroblasts) within the alveolar bone and/or the salivary glands ( 44 , 45 ). ALP can be a salivary biomarker of periodontal diseases and caries ( 46 ), as it interferes in the balance of the remineralization-demineralization cycle since it is primarily involved in calcium and phosphate binding ( 47 ).…”

Assessment of the effect of silica calcium phosphate nanocomposite on mesenchymal stromal cell differentiation and bone regeneration in critical size defect

“…The expression of osteopontin, which is found in osteoblasts, osteocytes, and osteoclasts, was maintained until day 21, indicating ongoing bone remodeling activity ( Merry et al, 1993 ). Furthermore, ALP which initiates the mineralization process of the extracellular matrix, was upregulated on day 14 but declined thereafter ( Rodrigues et al, 2016 ). These results are in accordance with the findings of Aniket, who found a similar pattern of ALP expression on day 14 ( Aniket et al, 2015 ).…”