Kinetics of expression of herpes simplex virus type 1-specific glycoprotein species on the surfaces of infected murine, simian, and human cells: flow cytometric analysis

Jennings, Stephen R.; Lippe, P A; Pauza, K J; Spear, Patricia G.; Pereira, Lenore; Tevethia, Satvir S.

doi:10.1128/jvi.61.1.104-112.1987

Cited by 34 publications

(11 citation statements)

References 45 publications

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“…51Cr-labeled sheep erythrocytes were sensitized with antierythrocyte IgG (EAIgG) and used in a rosetting assay to quantitate binding to the HSV-1 FcR expressed on infected cells. These studies were performed at 18 h postinfection, at a point when gCl, gDl, and the FcR are abundantly expressed on the cell surface (24). Figure la demonstrates the typical rosetting pattern observed when human umbilical vein endothelial cells were infected with a clinical isolate of HSV-1, strain NS.…”

Section: Resultsmentioning

confidence: 99%

A novel function of the herpes simplex virus type 1 Fc receptor: participation in bipolar bridging of antiviral immunoglobulin G

Frank

Friedman

1989

J Virol

150

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We describe a novel function of the Fc receptor of herpes simplex virus type 1 (HSV-1), its ability to participate in antibody bipolar bridging. This refers to the binding of a single immunoglobulin G (IgG) molecule by its Fab end to its antigenic target and by its Fc end to an Fc receptor (FcR). We demonstrate that various immune IgG antibodies, including polyclonal rabbit antibodies to HSV-1 glycoproteins gCl and gDl and monoclonal human antibody to gDl blocked rosetting of IgG-coated erythrocytes at IgG concentrations 100to 2,000-fold lower than required for rosette inhibition with nonimmune IgG. Steric hindrance did not account for the observed differences between immune and nonimmune IgG since rabbit anti-gC1 F(ab')2 fragments did not block rosetting. Murine anti-gCl or anti-gD1 IgG, a species of IgG incapable of binding by its Fc end to the HSV-1 FcR, also did not block rosetting. When cells were infected with a gCl-deficient mutant, anti-gCl IgG inhibited rosetting to the same extent as nonimmune IgG. This indicates that binding by the Fab end of the IgG molecule was required for maximum inhibition of rosetting. Bipolar bridging was shown to occur even when small concentrations of immune IgG were present in physiologic concentrations of nonimmune IgG. The biologic relevance of antibody bipolar bridging was evaluated by comparing antibodyand complement-dependent virus neutralization of an FcR-negative mutant and its parent HSV-1 strain. By engaging the Fc end of antiviral IgG, the parent strain resisted neutralization mediated by the classical complement pathway. These observations provide insight into the role of the HSV-1 FcR in pathogenesis and may help explain the function of FcR detected on other microorganisms.

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Section: Resultsmentioning

confidence: 99%

A novel function of the herpes simplex virus type 1 Fc receptor: participation in bipolar bridging of antiviral immunoglobulin G

Frank

Friedman

1989

J Virol

150

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“…The envelope is essential for infectivity, and maximal glycoprotein expression corresponds with maximal levels of infectious virus isolated from cells (38, 39). Confirming the present immunocytochemical results, different expression of HSV‐1 glycoproteins depending on the origin of the host cells was observed both by flow cytometric analysis (39) and immunoscanning electron microscopy (40) together with an always higher labelling density of gD than that of gC (40). In contrast to gD‐1 (41), abundant expression of gC‐1 is cytotoxic (42).…”

Section: Discussionmentioning

confidence: 99%

The morphogenesis of herpes simplex virus type 1 in infected parental mouse L fibroblasts and mutant gro29 cells

Jensen

Norrild

2003

APMIS

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Mutants of cell lines and viruses are important biological tools. The pathway of herpesvirus particle maturation and egress are contentious issues. The mutant gro29 line of mouse L cells is defective for egress of herpes simplex virus type 1 (HSV-1) virions, and a candidate for studies of virus-cell interactions. The properties of uninfected and HSV-1-infected L fibroblasts and gro29 cells investigated by protein assay, immunoblot, titration assay, immunofluorescence light microscopy and immunogold cryosection electron microscopy are reported. The ultrastructure of both HSV-1-infected L and gro29 cells confirmed primary envelopment of virions at the nuclear membranes followed by maturing multiple de-envelopments and re-envelopments in the endoplasmic reticulum and in the Golgi complex. The gro29 cells presented changed cytoskeleton, abolished egress of virions, and were defective in the trafficking of glycoproteins, giving rise to accumulation of viral particles and glycoproteins in the endoplasmic reticulum and the Golgi complex. The results suggest that gro29 cells harbour a causal underlying defect of the cytoskeleton in addition to the HSV-1-induced cytoskeletal changes.

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“…In all of these studies, virus-infected cells were treated with antibody to the viral antigen in question, followed by an FITC-labeled second antibody, and then surface glycoprotein expression on cells was detected and quantitated by flow cytometry. In addition to determining the presence or absence of viral proteins on the cell surface, some of these flow cytometric studies include data on the kinetics of expression of the different viral glycoproteins, an analysis that would be impossible to do by fluorescence microscopy (90). The ability to readily quantitate the number of cells expressing surface viral antigens and the relative amount of antigen per cell demonstrates the advantage of using flow cytometry over fluorescence microscopy, which yields only qualitative data.…”

Section: Detection Of Viral Antigens On the Cell Surfacementioning

confidence: 99%

Uses of flow cytometry in virology.

McSharry

1994

Clinical Microbiology Reviews

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these will be critically reviewed. The application of quantitative flow cytometry in the analysis of many other virus-infected cell systems is less well defined; however, that literature will be reviewed to demonstrate the broad applications of this technology for detecting and quantitating virus-infected cells. BRIEF DESCRIPTION OF FLOW CYTOMETRY Cytometry can be defined as the measurement of physical and/or chemical properties of cells. These measurements are often performed with a light or fluorescence microscope. Microscopy is qualitative but, with the exception of computerized image analysis (77), seldom quantitative. Flow cytometry is the measurement of the physical and/or chemical characteristics of cells while they pass single file in a fluid stream through a measuring apparatus (185). The fluorescence-activated cell sorter (FACS) was developed in the early 1970s to study isolated populations of viable cells for immunology. The original instruments were large, contained powerful dual lasers that were water cooled, and were able to analyze and sort cells. Although flow cytometry continues to function in the field of immunology, its use in other fields such as cell biology, 576

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Kinetics of expression of herpes simplex virus type 1-specific glycoprotein species on the surfaces of infected murine, simian, and human cells: flow cytometric analysis

Cited by 34 publications

References 45 publications

A novel function of the herpes simplex virus type 1 Fc receptor: participation in bipolar bridging of antiviral immunoglobulin G

A novel function of the herpes simplex virus type 1 Fc receptor: participation in bipolar bridging of antiviral immunoglobulin G

The morphogenesis of herpes simplex virus type 1 in infected parental mouse L fibroblasts and mutant gro29 cells

Uses of flow cytometry in virology.

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