Kinetics of cytochrome c oxidase from R. sphaeroides initiated by direct electron transfer followed by tr-SEIRAS

Steininger, Christoph; Reiner‐Rozman, Ciril; Schwaighofer, Andreas; Knoll, Wolfgang; Naumann, Renate

doi:10.1016/j.bioelechem.2016.06.005

Cited by 13 publications

(11 citation statements)

References 49 publications

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“…This inhibition regulates the conversion of available serine to siderophore, enterobactin, which thereby increases iron acquisition (Salvail et al, 2010 ). The cytochrome c oxidase (Cco) family related to oxidation-reduction was also inhibited in response to iron limitation, which is comprised of four subunits, CcoN, CcoO, CcoP, and CcoQ, which act as the terminal enzyme of respiratory chain (Ahn et al, 2015 ; Steininger et al, 2016 ). The Cco family, a member of heme-copper oxidase superfamily, may play a role in iron- restricted conditions.…”

Section: Discussionmentioning

confidence: 99%

The Role of the Regulator Fur in Gene Regulation and Virulence of Riemerella anatipestifer Assessed Using an Unmarked Gene Deletion System

Guo

et al. 2017

Front. Cell. Infect. Microbiol.

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Riemerella anatipestifer, an avian pathogen, has resulted in enormous economic losses to the duck industry globally. Notwithstanding, little is known regarding the physiological, pathogenic and virulence mechanisms of Riemerella anatipestifer (RA) infection. However, the role of Ferric uptake regulator (Fur) in the virulence of R. anatipestifer has not, to date, been demonstrated. Using a genetic approach, unmarked gene deletion system, we evaluated the function of fur gene in the virulence of R. anatipestifer. For this purpose, we constructed a suicide vector containing pheS as a counter selectable marker for unmarked deletion of fur gene to investigate its role in the virulence. After successful transformation of the newly constructed vector, a mutant strain was characterized for genes regulated by iron and Fur using RNA-sequencing and a comparison was made between wild type and mutant strains in both iron restricted and enriched conditions. RNA-seq analysis of the mutant strain in a restricted iron environment showed the downregulation and upregulation of genes which were involved in either important metabolic pathways, transport processes, growth or cell membrane synthesis. Electrophoretic mobility shift assay was performed to identify the putative sequences recognized by Fur. The putative Fur-box sequence was 5′-GATAATGATAATCATTATC-3′. Lastly, the median lethal dose and histopathological investigations of animal tissues also illustrated mild pathological lesions produced by the mutant strain as compared to the wild type RA strain, hence showing declined virulence. Conclusively, an unmarked gene deletion system was successfully developed for RA and the role of the fur gene in virulence was explored comprehensively.

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Section: Discussionmentioning

confidence: 99%

The Role of the Regulator Fur in Gene Regulation and Virulence of Riemerella anatipestifer Assessed Using an Unmarked Gene Deletion System

Guo

et al. 2017

Front. Cell. Infect. Microbiol.

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“…It was recently found that the measured average SEIRA signal of slits is higher compared to their inverse rod structures for the same vibrational layer, showing their advantages for SEIRA experiments over the better-known solid nanoantennas [7,8]. Moreover, slits present yet another advantage with respect to rods which makes them best suited for the present work: the gold surface surrounding the slits remains conducting and allows combining SEIRA with electrochemical measurements, which is of great interest to study redox proteins [9,10].…”

Section: Introductionmentioning

confidence: 93%

Surface Enhanced Infrared Characterization Using Disordered Slit-Antenna Arrays for the Detection of Electrodeposited Cytochrome C

et al. 2021

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An approach which allows both electrochemical studies and surface enhanced infrared characterization of electrodeposited Cytochrome C is presented. This approach is based on in-plane disordered arrays of resonant slits engraved in Au substrates using focused ion beam. For light-polarized perpendicular to the slit, the reflectivity spectra of the slit arrays show dips related to the excitation of the slit plasmon resonance, whose position depends on the slit length. Due to the presence of the continuous Au layer around the slits, the very same substrates can be used to perform electrochemical studies. By varying the slit length, we have tuned the plasmon resonance to match the absorption bands of electrodeposited Cytochrome C, demonstrating the detection of minute amounts of this protein, all the way down to a single monolayer.

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“…The process is coupled to proton translocation across the membrane. Steininger et al used TR-SEIRA to investigate the conformational changes of R. sphaeroides CcO occurring in the ms timescale during the enzymatic turnover, i.e., in the presence of O 2 , and compared these results with previous studies under anaerobic conditions .…”

Section: Spectroelectrochemical Case Studiesmentioning

confidence: 99%

In Situ Spectroelectrochemical Investigations of Electrode-Confined Electron-Transferring Proteins and Redox Enzymes

Murgida

2021

ACS Omega

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This perspective analyzes recent advances in the spectroelectrochemical investigation of redox proteins and enzymes immobilized on biocompatible or biomimetic electrode surfaces. Specifically, the article highlights new insights obtained by surface-enhanced resonance Raman (SERR), surfaceenhanced infrared absorption (SEIRA), protein film infrared electrochemistry (PFIRE), polarization modulation infrared reflection−absorption spectroscopy (PMIRRAS), Forster resonance energy transfer (FRET), X-ray absorption spectroscopy (XAS), electron paramagnetic resonance (EPR), and differential electrochemical mass spectrometry (DMES)-based spectroelectrochemical methods on the structure, orientation, dynamics, and reaction mechanisms for a variety of immobilized species. This includes small heme and copper electron shuttling proteins, large respiratory complexes, hydrogenases, multicopper oxidases, alcohol dehydrogenases, endonucleases, NO-reductases, and dye decolorizing peroxidases, among other enzymes. Finally, I discuss the challenges and foreseeable future developments toward a better understanding of the functioning of these complex macromolecules and their exploitation in technological devices.

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Kinetics of cytochrome c oxidase from R. sphaeroides initiated by direct electron transfer followed by tr-SEIRAS

Cited by 13 publications

References 49 publications

The Role of the Regulator Fur in Gene Regulation and Virulence of Riemerella anatipestifer Assessed Using an Unmarked Gene Deletion System

The Role of the Regulator Fur in Gene Regulation and Virulence of Riemerella anatipestifer Assessed Using an Unmarked Gene Deletion System

Surface Enhanced Infrared Characterization Using Disordered Slit-Antenna Arrays for the Detection of Electrodeposited Cytochrome C

In Situ Spectroelectrochemical Investigations of Electrode-Confined Electron-Transferring Proteins and Redox Enzymes

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