Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides

Shen, Hongwei; Gong, Zhiwei; Yang, Xiaobing; Jin, Guojie; Bai, Feng‐Wu; Zhao, Zongbao K.

doi:10.1016/j.jbiotec.2013.08.010

Cited by 65 publications

(51 citation statements)

References 28 publications

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“…Between the two tested species, R. babjevae seems to be the yeast of choice in terms of further research for biofuel production from lignocellulose. The obtained lipid yield- and -production rate were also relatively high compared to other oleaginous yeasts, cultivated on either lignocellulosic hydrolysate or a glucose-based model substrate (Slininger et al 2016, Shen et al 2013). In recent system analyses, we pointed out that rapid conversion of the substrate to lipids has great impact on the sustainability of the conversion, because aerobic cultivations, as required for lipid production, require much energy, implying that a faster fermentation will largely improve process efficiency (Karlsson et al 2016, Karlsson et al 2017).…”

Section: Discussionmentioning

confidence: 77%

Bioethanol and lipid production from the enzymatic hydrolysate of wheat straw after furfural extraction

Brandenburg

Poppele

Blomqvist

et al. 2018

Appl Microbiol Biotechnol

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This study investigates biofuel production from wheat straw hydrolysate, from which furfural was extracted using a patented method developed at the Latvian State Institute of Wood Chemistry. The solid remainder after furfural extraction, corresponding to 67.6% of the wheat straw dry matter, contained 69.9% cellulose of which 4% was decomposed during the furfural extraction and 26.3% lignin. Enzymatic hydrolysis released 44% of the glucose monomers in the cellulose. The resulting hydrolysate contained mainly glucose and very little amount of acetic acid. Xylose was not detectable. Consequently, the undiluted hydrolysate did not inhibit growth of yeast strains belonging to Saccharomyces cerevisiae, Lipomyces starkeyi, and Rhodotorula babjevae. In the fermentations, average final ethanol concentrations of 23.85 g/l were obtained, corresponding to a yield of 0.53 g ethanol per g released glucose. L. starkeyi generated lipids with a rate of 0.08 g/h and a yield of 0.09 g per g consumed glucose. R. babjevae produced lipids with a rate of 0.18 g/h and a yield of 0.17 per g consumed glucose. In both yeasts, desaturation increased during cultivation. Remarkably, the R. babjevae strain used in this study produced considerable amounts of heptadecenoic, α,- and γ-linolenic acid.

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Section: Discussionmentioning

confidence: 77%

Bioethanol and lipid production from the enzymatic hydrolysate of wheat straw after furfural extraction

Brandenburg

Poppele

Blomqvist

et al. 2018

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

show abstract

“…Lower total lipid cell content and lipid productivity were expected in continuous cultures (the cells are in the exponential growth phase) because of the nitrogen impact on lipid accumulation and cell growth. Many studies showed that microorganisms accumulate reserve materials including lipids when they are grown at lower dilution rates, limited usually by nitrogen source . The cultivations were performed at medium dilution rate of 0.1 h −1 in order to determine adequate conditions for lipid accumulation and cell growth.…”

Section: Resultsmentioning

confidence: 99%

“…During continuous culture, cells are in the exponential growth phase and consequently they need nitrogen source to insure synthesis of cell building blocks. However, Evans and Ratledge and Shen at al . obtained relatively high lipid content in continuous cultures of oleaginous yeasts at relatively low dilution rates, which is comparable with lipid content in batch cultures.…”

Section: Introductionmentioning

confidence: 93%

Effect of carbon and nitrogen source concentrations on the growth and lipid accumulation of yeastTrichosporon oleaginosusin continuous and batch culture

Šantek

Miškulin

Petrović

et al. 2016

J. Chem. Technol. Biotechnol

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BACKGROUND Biodiesel is an alternative to conventional fossil diesel fuel. Currently, it is mainly produced from vegetable oils and consequently it has an adverse impact on food price. Lipids from oleaginous microorganisms (e.g. microalgae, bacteria, fungi and yeasts) could be an alternative feedstock for biodiesel production and therefore the growth and lipid accumulation of Trichosporon oleaginosus was studied under different conditions. RESULTS The growth of T. oleaginosus on the xylose containing media enhanced the lipid accumulation and reduced cell growth, while on the glucose‐containing media the opposite effect was observed. Trichosporon oleaginosus can accumulate above 50% of total lipids in dry cell mass during continuous and batch culture in the nitrogen‐limited conditions. In the continuous culture relatively high productivity of total lipid accumulation (0.67 g dm−3 h−1) was observed on the glucose containing media. The type of substrate limitation has considerable impact on the fatty acid composition of yeast cell lipids. The predicted biodiesel properties (based on the fatty acids composition of lipids) were found to meet the CEN quality standards. CONCLUSION The results obtained show that T. oleaginosus has great potential for feasible biodiesel production, especially when sugars from industrial and agricultural waste are used as a substrate. © 2016 Society of Chemical Industry

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“…30 This could be a possible reason why the culture started washing out of the bioreactor when the dilution rate was increased from 1 to 1.25 day −1 . From Eqn (4) this corresponds to a specific growth rate of 1 day −1 or 0.042 h −1 as it is well known that a culture cannot grow faster than its maximum specific growth rate.…”

Section: Development Of Continuous Cultivation Process For Biomass Anmentioning

confidence: 99%

Development of continuous cultivation process for oil production through bioconversion of minimally treated waste streams from second‐generation bioethanol production

Singh

Mehta

Saxena

et al. 2018

J of Chemical Tech & Biotech

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BACKGROUND Efficient conversion of inhibitor containing pentose rich stream into bioethanol or biogas is still challenging due to several technological challenges; however, this stream can be efficiently assimilated into lipids by oleaginous yeasts such as Rhodotorula. In this work, a continuous and nonsterile process was developed for bioconversion of waste streams into lipids for biodiesel using growth‐inhibitor‐tolerant oleaginous yeast. RESULTS An inhibitor‐tolerating property of the strain was used to develop a cyclic and continuous near axenic cultivation process in a 2 L nonsterile bioreactor to enhance valorization of waste streams and to reduce energy requirements for media and vessel sterilization. After media optimization, continuous cultivation resulted in significant increase in biomass (14.90 g L−1 day−1) and lipid productivity (6.15 g L−1 day−1) as compared to flask (biomass ∼3 g L−1 day−1 and lipid productivity ∼0.6 g L−1 day−1) results. Palmitic acid (C16:0) and oleic acid (C18:1) were the major fatty acids, accounting for almost 80% of total lipids, thus making lipid produced in the process suitable for biodiesel production. CONCLUSION Inhibitors present in waste streams successfully maintained near axenic conditions during the cultivation. Maximum dilution rate of 1 was achieved after media optimization, which resulted in complete valorization of nutrients into biomass and lipids with high productivity. © 2018 Society of Chemical Industry

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Kinetics of continuous cultivation of the oleaginous yeast Rhodosporidium toruloides

Cited by 65 publications

References 28 publications

Bioethanol and lipid production from the enzymatic hydrolysate of wheat straw after furfural extraction

Bioethanol and lipid production from the enzymatic hydrolysate of wheat straw after furfural extraction

Effect of carbon and nitrogen source concentrations on the growth and lipid accumulation of yeastTrichosporon oleaginosusin continuous and batch culture

Development of continuous cultivation process for oil production through bioconversion of minimally treated waste streams from second‐generation bioethanol production

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