Kinetics of BSA release from poly(N-isopropylacrylamide) hydrogels

Naddaf, A.A.; Tsibranska, I.; Bart, H.-J.

doi:10.1016/j.cep.2010.05.008

Cited by 25 publications

(18 citation statements)

References 40 publications

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“…In [38], the authors followed the release of BSA from previously dried poly(N-isopropylacrylamide) hydrogels, which lasted barely 2 h at 40ºC, and obtained higher values for the k and n parameters of 0.112 and 0.462, respectively. Other BSA-gel systems have also been found to exhibit double kinetics of BSA release, which have been interpreted as an indication of the interference with diffusion-controlled transport of further mechanisms: swelling or shrinking of the gel matrix (sometimes triggered by pH or temperature changes in the environment) [38,39,41], chemical reactions (polymer degradation, drug-polymer interaction, etc.) [36,39,42], and processing (heterogeneous cross-linking of the gel, entrapment of the drug on the surface, use of solvents afterwards dried, etc.)…”

Section: Discussionmentioning

confidence: 99%

“…leading to an uneven drug distribution [43]. When hydrogel samples are dried after the protein loading, their swelling usually provokes a burst release [36,38], due to those molecules near the polymer surface and rapidly transferred to the medium. This is obviously not the case here, as the constructs were not dried after the protein loading.…”

Section: Discussionmentioning

confidence: 99%

“…The fitting of the power law to the second release regime gives n = 1 (linear growth with time of the amount released) and k = 0.0004 (plus the new parameter = 0.1493 needed to account for the initial release). Power laws such as the stated one are typically used to describe solvent release from hydrogels [38][39][40]; the parameter k is then interpreted as a structural/geometric factor to account for the different tortuosities of the transport path. In [38], the authors followed the release of BSA from previously dried poly(N-isopropylacrylamide) hydrogels, which lasted barely 2 h at 40ºC, and obtained higher values for the k and n parameters of 0.112 and 0.462, respectively.…”

Section: Discussionmentioning

confidence: 99%

“…Power laws such as the stated one are typically used to describe solvent release from hydrogels [38][39][40]; the parameter k is then interpreted as a structural/geometric factor to account for the different tortuosities of the transport path. In [38], the authors followed the release of BSA from previously dried poly(N-isopropylacrylamide) hydrogels, which lasted barely 2 h at 40ºC, and obtained higher values for the k and n parameters of 0.112 and 0.462, respectively. Other BSA-gel systems have also been found to exhibit double kinetics of BSA release, which have been interpreted as an indication of the interference with diffusion-controlled transport of further mechanisms: swelling or shrinking of the gel matrix (sometimes triggered by pH or temperature changes in the environment) [38,39,41], chemical reactions (polymer degradation, drug-polymer interaction, etc.)…”

Section: Discussionmentioning

confidence: 99%

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Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

Vallés-Lluch¹,

Arnal-Pastor²,

Martínez‐Ramos³

et al. 2013

Acta Biomaterialia

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ElsevierVallés Lluch, A.; Arnal Pastor, MP.; Martínez Ramos, C.; Vilariño Feltrer, G.; Vikingsson, L.; Castells Sala, C.; Semino, CE.... (2013). Combining self-assembling peptide gels with three-dimensional elastomer scaffolds. Acta Biomaterialia. 9 (12) Abstract: Some of the problems raised by the combination of porous scaffolds and self-assembling peptide (SAP) gels as constructs for tissue engineering applications are for the first time addressed. Scaffolds of poly(ethyl acrylate) and the SAP gel RAD16-I were employed. The in situ gelation of the SAP gel inside the pores of the scaffolds was studied. The scaffold-cum-gel constructs were characterized morphologically, physico-chemically and mechanically. The possibility of incorporating an active molecule (bovine serum albumin, taken here as a model molecule for others) in the gel within the scaffold's pores was assessed, and the kinetics of its release in PBS was followed. Cell seeding and colonization of these constructs was preliminary studied with L929 fibroblasts and checked afterwards with sheep adipose-tissue derived stem cells (ASCs) intended for further preclinical studies. Static (conventional) and dynamically assisted seedings were compared for bare scaffolds and the scaffoldcum-gel constructs. The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the 3D structure. These constructs could be of use in different advanced tissue engineering applications where, apart from a cell-friendly ECM-like aqueous environment, a larger-scale three-dimensional structure able to keep the cells in a specific place, give mechanical support and/or conduct spatially the tissue growth could be required. COMBINING SELF-ASSEMBLING PEPTIDE GELS WITH 3D ELASTOMER SCAFFOLDSA. AbstractSome of the problems raised by the combination of porous scaffolds and selfassembling peptide (SAP) gels as constructs for tissue engineering applications are for the first time addressed. Scaffolds of poly(ethyl acrylate) and the SAP gel RAD16-I were employed. The in situ gelation of the SAP gel inside the pores of the scaffolds was studied. The scaffold-cum-gel constructs were characterized morphologically, physicochemically and mechanically. The possibility of incorporating an active molecule (bovine serum albumin, taken here as a model molecule for others) in the gel within the scaffold's pores was assessed, and the kinetics of its release in PBS was followed. Cell intended for further preclinical studies. Static (conventional) and dynamically assisted seedings were compared for bare scaffolds and the scaffold-cum-gel constructs. The SAP gel inside the pores of the scaffold significantly improved the uniformity and density of cell colonization of the 3D structure. These constructs could be of use in different advanced tissue engineering applications where, apart from a cell-friendly ECM-like aqueous environment, a larger-scale three-dimensional structure able to keep the cells in a specific place, give mechanical support and/o...

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

Vallés-Lluch¹,

Arnal-Pastor²,

Martínez‐Ramos³

et al. 2013

Acta Biomaterialia

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“…A good fit of the first stage of the time-release curve to a power law was When hydrogel samples are dried after the protein loading, their swelling usually provokes a burst release [311,312], due to those molecules near the polymer surface and rapidly transferred to the medium. This is obviously not the case here, as the constructs were not dried after the protein loading.…”

mentioning

confidence: 99%

New scaffolding materials for the regeneration of infarcted myocardium

Pastor¹,

Pilar²

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Hydroxyapatite sonosensitization of ultrasound‐triggered, thermally responsive hydrogels: An on‐demand delivery system for bone repair applications

et al. 2021

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While bones have the innate capability to physiologically regenerate, in certain cases regeneration is suboptimal, too slow, or does not occur. Biomaterials‐based growth factor delivery systems have shown potential for the treatment of challenging bone defects, however, achieving controlled growth factor release remains a challenge. The objective of this study was to develop a thermally responsive hydrogel for bone regeneration capable of ultrasound‐triggered on‐demand delivery of therapeutic agents. Furthermore, it was hypothesized that incorporation of hydroxyapatite (HA) into the hydrogel could increase sonosensitization, augmenting ultrasound sensitivity to enable controlled therapeutic release to the target tissue. Alginate thermally responsive P(Alg‐g‐NIPAAm) hydrogels were fabricated and varying quantities of HA (1, 3, 5, and 7% wt./vol.) incorporated. All hydrogels were highly injectable (maximum injection force below 6.5 N) and rheological characterization demonstrated their ability to gel at body temperature. The study demonstrated the ultrasound‐triggered release of sodium fluorescein (NaF), bovine serum albumin (BSA), and bone morphogenetic protein 2 (BMP‐2) from the hydrogels. Release rates of BSA and BMP‐2 were significantly enhanced in the HA containing hydrogels, confirming for the first time the role of HA as a son sensitizer. Together these results demonstrate the potential of these ultrasound‐triggered thermally responsive hydrogels for on‐demand delivery of therapeutic agents for bone regeneration.

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Kinetics of BSA release from poly(N-isopropylacrylamide) hydrogels

Cited by 25 publications

References 40 publications

Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

Combining self-assembling peptide gels with three-dimensional elastomer scaffolds

New scaffolding materials for the regeneration of infarcted myocardium

Hydroxyapatite sonosensitization of ultrasound‐triggered, thermally responsive hydrogels: An on‐demand delivery system for bone repair applications

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