Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry

Healy, Kevin E.; Thomas, Carson H.; Rezania, Alireza; Kim, Jung E.; McKeown, P.J.; Lom, Barbara; Hockberger, Philip E.

doi:10.1016/0142-9612(96)85764-4

Cited by 245 publications

(160 citation statements)

References 46 publications

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“…These results highlight the role of surface chemistry in organizing cells and forming mineralized tissue in vitro. 272 To study adhesion, spreading, and focal contact formation of bone cells, Rezania et al 273 immobilized a 15-aminoacid peptide that contained an RGD (Arg-Gly Asp) sequence unique to bone sialoprotein. The peptide surfaces were fabricated by using a heterobifunctional crosslinker to link the peptide to amine-functionalized quartz surfaces.…”

Section: Vg Bone Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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The ability to culture cells in vitro has revolutionized hypothesis testing in basic cell and molecular biology research and has become a standard methodology in drug screening and toxicology assays. However, the traditional cell culture methodology-consisting essentially of the immersion of a large population of cells in a homogeneous fluid medium-has become increasingly limiting, both from a fundamental point of view (cells in vivo are surrounded by complex spatiotemporal microenvironments) and from a practical perspective (scaling up the number of fluid handling steps and cell manipulations for high-throughput studies in vitro is prohibitively expensive). Micro fabrication technologies have enabled researchers to design, with micrometer control, the biochemical composition and topology of the substrate, the medium composition, as well as the type of neighboring cells surrounding the microenvironment of the cell. In addition, microtechnology is conceptually well suited for the development of fast, low-cost in vitro systems that allow for high-throughput culturing and analysis of cells under large numbers of conditions. Here we review a variety of applications of microfabrication in cell culture studies, with an emphasis on the biology of various cell types.

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Section: Vg Bone Cell Biology On a Chipmentioning

confidence: 99%

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Tourovskaia

Folch

2003

Crit Rev Biomed Eng

173

140

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“…Compared to bioactive glass, silicon dioxides are quite tissue-unfriendly (Hench and Wilson, 1986;Liu et al, 2004), inhibiting their use in devices designed to be integrated with the human body. It was hypothesized that cytocompatibility of the silicon-based materials could be enhanced by only partial surface micro-texturing with well-known biocompatible materials: cell adhesion and spreading of the cells were improved (p<0.001 for both biomaterial vs. silicon comparisons).…”

Section: Discussionmentioning

confidence: 99%

“…The physical and chemical properties of a material control which molecules are bound and at what concentrations, their ratios and the orientation of adsorption on the surface (Boyan et al, 1996;Healy et al, 1996;Keselowsky et al, 2003). In addition, surface topography at the nano-and microscales influences several aspects of cell behaviour (reviewed by Flemming et al, 1999).…”

Section: Introductionmentioning

confidence: 99%

Enhancement of silicon using micro-patterned surfaces of thin films

Kaivosoja

Myllymaa²,

Kouri³

et al. 2010

eCM

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Micro-textured biomaterials might enhance cytocompatibility of silicon-based micro-electromechanical system (bio-MEMS) dummies. Photolithography-physical vapour deposition was used to produce diamond-like carbon (DLC) or Ti squares and circles on silicon, and also their inverse replicas; then DLC and Ti were compared for their guiding potential, using a SaOS-2 cell model. Scanning electron microscopy at 48 hours indicated cells were well-spread on large-sized patterns (several cells on one pattern) and assumed the geometrical architecture of underlying features. Mediumsized patterns (slightly smaller than solitary indicator cells) were inhabited by singular cells, which stretched from one island to another, assuming longitudinal or branching morphologies. On small-sized patterns (much smaller than individual cells) cells covered large micro-textured areas, but cellular filopodia bypassed the bare silicon. Immunofluorescence and confocal laser scanning microscopy indicated that the actin cytoskeleton and vinculin-containing adhesion junctions were present on the patterned areas, but not on the bare silicon. Cell density/ coverage disclosed a 3.4-3.7-fold preference for the biomaterial patterns over silicon substrate (p < 0.001). Differences in the cellular response between materials were lost at 120 hours when cells were confluent. The working hypothesis was proven; enhancement by micro-patterning depends on the pattern size, shape and material and can be used to improve biocompatibility during the initial integration phase of the device.

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“…It was reported that the interaction between a biomaterial and MG-63 cells depends on the topography [49][50][51][52][53], wettability [53,54] and surface energy and charge [55,56] of the biomaterial surface. We assumed that the cell adhesion was suppressed by the surface structure of HAp-coated HNS during the initial stage of the culture.…”

Section: Mg-63 Cell Culture On Cap-coated Hnsmentioning

confidence: 99%

Preparation and biological evaluation of hydroxyapatite-coated nickel-free high-nitrogen stainless steel

Sasaki

Inoue

Katada

et al. 2012

Science and Technology of Advanced Materials

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Calcium phosphate was formed on nickel-free high-nitrogen stainless steel (HNS) by chemical solution deposition. The calcium phosphate deposition was enhanced by glutamic acid covalently immobilized on the surface of HNS with trisuccinimidyl citrate as a linker. X-ray diffraction patterns and Fourier transform infrared spectra showed that the material deposited on glutamic acid-immobilized HNS within 24 h was low-crystallinity calcium-deficient carbonate-containing hydroxyapatite (HAp). The biological activity of the resulting HAp-coated HNS was investigated by using a human osteoblast-like MG-63 cell culture. The HAp-coated HNS stimulated the alkaline-phosphate activity of the MG-63 culture after 7 days. Therefore, HAp-coated HNS is suitable for orthopedic devices and soft tissue adhesion materials.

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Kinetics of bone cell organization and mineralization on materials with patterned surface chemistry

Cited by 245 publications

References 46 publications

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Biology on a Chip: Microfabrication for Studying the Behavior of Cultured Cells

Enhancement of silicon using micro-patterned surfaces of thin films

Preparation and biological evaluation of hydroxyapatite-coated nickel-free high-nitrogen stainless steel

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