Kinetics of appearance of hemorphins from bovine hemoglobin peptic hydrolysates by a direct coupling of reversed-phase high-performance liquid chromatography and electrospray ionization mass spectrometry

Ion-pairing separation of bioactive peptides using an aqueous/octan-1-ol micro-extraction system from bovine haemoglobin complex hydrolysates

“…These peptides contained an arginine basic residue in their sequence and had a hydrophobicity of about 1700 cal/mol, as the Bigelow scale [27], which was a high value compared with the average hydrophobicity of peptides produced during haemoglobin hydrolysis by pepsin which is about 1000 cal/mol. Moreover, these haemorphins were present in some very complex peptidic mixtures which contained more than hundreds of peptides with different sequence [29]. Fig.…”

Transformation of antimicrobial into bradykinin‐potentiating peptides during peptic hydrolysis of bovine haemoglobin: identification, release kinetics and reaction network of peptides

“…16 In the present work the biotransformation process from the antimicrobial peptide α107-136 to the bradykinin-potentiating peptide α110-125 was extensively investigated during peptic hydrolysis of bovine haemoglobin. Compared with the precursor haemorphin LVVH-7 (β31-40) studied previously, 7,8 the antibacterial peptide α107-136 has many more cleavage sites for pepsin in the sequence. It is for this reason that the diversity of the reaction components in our experiment is much greater than that in earlier studies.…”

“…Such was the case with the biotransformation of one haemorphin to another in previous studies. 7,8 These haemorphins were all found in the 31-40 region of the bovine haemoglobin β-chain. Their release kinetics was typically followed by liquid chromatography coupled with either photodiode array detection 7 or mass spectrometry.…”

“…Their release kinetics was typically followed by liquid chromatography coupled with either photodiode array detection 7 or mass spectrometry. 8 During the last decade, two types of bioactive peptides, namely antimicrobial and antihypertensive peptides, have been mostly studied by bulk experiments. Antimicrobial peptides were considered as an evident component in the innate immunity system of vertebrates to prevent microbial invasion.…”

Advancement of foam separation of bioactive peptides using an aeration column with a bubbling-draining method

“…Experimental procedure of peptidic hydrolysates coupled RP-HPLC-MS was performed according to the literature [10].…”