Kinetics of antibodies in sera, saliva, and urine samples from adult patients with primary or secondary dengue 3 virus infections

Vázquez, Susana; Cabezas, Sheila; Pérez, Ana B.; Pupo, Maritza; Ruiz, Didye; Calzada, Naifi; Bernardo, Lídice; Castro, Osvaldo; González, Daniel; Serrano, Teresita; Sánchez, Amaury Pérez; Guzmán, María G.

doi:10.1016/j.ijid.2006.05.005

Cited by 76 publications

(77 citation statements)

References 33 publications

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“…In our extensive experience with developing UTI vaccines, we have found that protection in the bladder is more difficult to achieve in our murine model than protection in the kidneys, likely due to low levels of antigen-specific antibody detected in the bladder (20,42). This may be due to technical issues implicit in a murine model of UTI or the lower abundance of antibodies in the urine compared with the sera (20,(58)(59)(60). Alternatively, the lack of protection in the bladder in response to targeting Aer-mediated iron acquisition could be due to the different immune response elicited by vaccination with the low-molecular mass siderophores compared with the proteinaceous receptors.…”

Section: Discussionmentioning

confidence: 99%

Siderophore vaccine conjugates protect against uropathogenicEscherichia coliurinary tract infection

Mike

Smith

Sumner

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Uropathogenic Escherichia coli (UPEC) is the primary cause of uncomplicated urinary tract infections (UTIs). Whereas most infections are isolated cases, 1 in 40 women experience recurrent UTIs. The rise in antibiotic resistance has complicated the management of chronic UTIs and necessitates new preventative strategies. Currently, no UTI vaccines are approved for use in the United States, and the development of a highly effective vaccine remains elusive. Here, we have pursued a strategy for eliciting protective immunity by vaccinating with small molecules required for pathogenesis, rather than proteins or peptides. Small iron-chelating molecules called siderophores were selected as antigens to vaccinate against UTI for this vaccine strategy. These pathogen-associated stealth siderophores evade host immune defenses and enhance bacterial virulence. Previous animal studies revealed that vaccination with siderophore receptor proteins protects against UTI. The poor solubility of these integral outer-membrane proteins in aqueous solutions limits their practical utility. Because their cognate siderophores are water soluble, we hypothesized that these bacterial-derived small molecules are prime vaccine candidates. To test this hypothesis, we immunized mice with siderophores conjugated to an immunogenic carrier protein. The siderophore-protein conjugates elicited an adaptive immune response that targeted bacterial stealth siderophores and protected against UTI. Our study has identified additional antigens suitable for a multicomponent UTI vaccine and highlights the potential use of bacterial-derived small molecules as antigens in vaccine therapies.vaccine conjugate | urinary tract infection | siderophore | UPEC | iron acquisition

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Section: Discussionmentioning

confidence: 99%

Siderophore vaccine conjugates protect against uropathogenicEscherichia coliurinary tract infection

Mike

Smith

Sumner

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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Section: Clinical Signs and Immunological Responsementioning

confidence: 99%

“…Compared to the haemagglutination inhibition assay as the gold standard, MAC-ELISA shows a sensitivity and specificity of 90% and 98%, respectively, in samples collected after 5 days of fever 55 . In addition to serum, dengue-specific IgM can be detected in whole blood on filter paper (sensitivity 98.1% and specificity 98.5%) 77,78 and in saliva (sensitivity 90.3% and specificity 92.0%) 79 , but not in urine 68 . More than 50 commercial kits are available with variable sensitivity and specificity 65,[80][81][82] .…”

Section: Mac-elisamentioning

confidence: 99%

“…Following a dengue infection, IgG can be lifelong, which complicates the serodiagnosis of past, recent and current infections 65,67 . IgA and IgE responses have also been documented but the utility of detecting these immunoglobulins as markers for dengue serodiagnosis requires further study 68 .In areas where two or more flaviviruses are circulating, multiple and sequential flavivirus infections make differential diagnosis difficult owing to the presence of pre-existing antibodies and the phenomenon of original antigenic sin (during sequential flavivirus infections, B-cell clones responding to the first infection synthesize antibodies with higher affinity for the first infecting virus than for the second infecting virus) 69 . …”

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confidence: 99%

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Dengue: a continuing global threat

et al. 2010

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Dengue fever and dengue haemorrhagic fever are important arthropod-borne viral diseases. Each year, there are ~50 million dengue infections and ~500,000 individuals are hospitalized with dengue haemorrhagic fever, mainly in Southeast Asia, the Pacific and the Americas. Illness is produced by any of the four dengue virus serotypes. A global strategy aimed at increasing the capacity for surveillance and outbreak response, changing behaviours and reducing the disease burden using integrated vector management in conjunction with early and accurate diagnosis has been advocated. Antiviral drugs and vaccines that are currently under development could also make an important contribution to dengue control in the future.Dengue is the most important arthropod-borne viral infection of humans. Worldwide, an estimated 2.5 billion people are at risk of infection, approximately 975 million of whom live in urban areas in tropical and sub-tropical countries in Southeast Asia, the Pacific and the Americas 1 . Transmission also occurs in Africa and the Eastern Mediterranean, and rural Europe PMC Funders GroupAuthor Manuscript Nat Rev Microbiol. Author manuscript; available in PMC 2015 February 19. Published in final edited form as:Nat Rev Microbiol. 2010 December ; 8(12 0): S7-16. doi:10.1038/nrmicro2460. Europe PMC Funders Author ManuscriptsEurope PMC Funders Author Manuscripts communities are increasingly being affected. It is estimated that more than 50 million infections occur each year, including 500,000 hospitalizations for dengue haemorrhagic fever, mainly among children, with the case fatality rate exceeding 5% in some areas [1][2][3][4] . The geographical areas in which dengue transmission occurs have expanded in recent years (FIG. 1), and all four dengue virus serotypes (DENV-1-4) are now circulating in Asia, Africa and the Americas, a dramatically different scenario from that which prevailed 20 or 30 years ago (FIG. 2). The molecular epidemiology of these serotypes has been studied in an attempt to understand their evolutionary relationships 11 .This Review will provide an update on our understanding of the pathogenesis of this successful pathogen, how we diagnose and control infection and the progress that has been made in vaccine development. Dengue virus pathogenesisDengue viruses belong to the genus flavivirus within the Flaviviridae family. DENV-1-4 evolved in non-human primates from a common ancestor and each entered the urban cycle independently an estimated 500-1,000 years ago 12 . The virion comprises a spherical particle, 40-50 nm in diameter, with a lipopolysaccharide envelope. The positive singlestrand RNA genome (FIG. 3), which is approximately 11 kb in length, has a single open reading frame that encodes three structural proteins -the capsid (C), membrane (M) and envelope (E) glycoproteins -and seven non-structural proteins (NS1, NS2A, NS2B, NS3, NS4A, NS4B and NS5 [18][19][20] . ADE occurs when mononuclear phagocytes are infected through their Fc receptors by immune complexes that form between DE...

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“…Diagnosis of other viral infections in urine and saliva samples has been reported for human immunodeficiency virus (HIV), Hepatitis A, B, and C, and rubella [14][15][16][17][18][19][20], and more recently in other flaviviruses, West-Nile virus (WNV) [21][22][23] and Dengue (DENV) serotypes -1, -2, -3, and -4 [24][25][26][27][28][29][30][31][32][33][34][35]. WNV can be detected longer in urine than serum [21] and can be isolated from urine [22,23].…”

Section: Advantages Of Non-invasive Sample Collectionsmentioning

confidence: 99%