Kinetics of albumin- and alpha-fetoprotein-production during rat liver development

Elmaouhoub, Abderrahim; Dudás, József; Ramadori, Giuliano

doi:10.1007/s00418-007-0338-y

Cited by 25 publications

(22 citation statements)

References 33 publications

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“…To determine whether the epithelioid cells in the freshly isolated HSC preparations were quiescent HSCs, we evaluated the cells for coexpression of albumin and quiescent HSC markers (desmin or GFAP), reasoning that albumin is a relatively specific marker for hepatocytes and their immediate precursors (45,46). Flow cytometry demonstrated coexpression of albumin and desmin in over 90% of the freshly isolated HSCs ( Figure 7C).…”

Section: Facs and Fate-mapping Evidence That Hscs And Mf-hscs Are Repmentioning

confidence: 99%

Smoothened is a master regulator of adult liver repair

Michelotti

Xie²,

Swiderska³

et al. 2013

J. Clin. Invest.

156

249

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Section: Facs and Fate-mapping Evidence That Hscs And Mf-hscs Are Repmentioning

confidence: 99%

Smoothened is a master regulator of adult liver repair

Michelotti

Xie²,

Swiderska³

et al. 2013

J. Clin. Invest.

156

249

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“…26 Briefly, mouse hepatocytes were kept in culture 24 h and successively labeled in methionin-free RPMI medium supplemented with 35 S-methionin (100 mCi/300 ml medium pro well) for 6 h. Part of the cells was further stimulated with murine IL-6 at a concentration of 100 ng/ml. After the labeling, cells were washed three times in PBS whereas the supernatants were collected and diluted in 50% lysis buffer containing 1% sodium dodecyl sulfate (SDS).…”

Section: Biosynthetic Labeling Immunoprecipitation and Nupage Analysmentioning

confidence: 99%

Cellular and molecular mechanisms regulating the hepatic erythropoietin expression during acute-phase response: a role for IL-6

Ramadori

Ahmad

Ramadori

2010

Laboratory Investigation

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The source of circulating erythropoietin (EPO), the mediators and the mechanisms involved in the upregulation of EPO gene expression during acute-phase reaction are still poorly understood. Acute-phase reaction was induced by either intramuscular turpentine oil (TO) or intraperitoneal lipopolysaccharide (LPS) administration into wild-type and interleukin (IL)-6 knockout (KO) mice. Animals were killed at different time points and blood, liver and muscle tissue were collected. Serum levels of EPO were measured by enzyme-linked immunoadsorbent assay; liver and injured muscle samples were processed for RNA isolation and for protein analysis. EPO, hypoxia-inducible factors 1a and 2a (HIF-1a and HIF-2a) mRNA were analyzed by RT-PCR and the protein levels were analyzed by western blot and electrophoretic mobility shift assay. HIF-1a and HIF-2a localization was performed through immunofluorescence staining. EPO, HIF-1 and HIF-2 gene and protein expression levels were also analyzed in isolated mouse hepatocytes after stimulation with IL-6. In the wild-type animals, EPO serum levels increased dramatically at 12 h after the insults together with the hepatic gene expression. In TO-treated animals, the EPO gene expression reached an 8.2-fold increase at 12 h, and in LPS-treated mice a similar induction was recorded at 6 h (about 4.5-fold increase). In the IL-6KO strain, the upregulation after the inflammatory stimuli was much lower (only 2.0-fold increase). A progressive upregulation of HIF-1a and HIF-2a was detectable until 6 h after the insults, but only HIF-1a upregulation was reduced in IL-6KO mice. In isolated hepatocytes, stimulation with a single dose of IL-6 induced a nuclear accumulation of HIF-1a, in parallel with an increase of EPO mRNA. No effect on HIF-2a expression was found. IL-6 appears to be the main regulator of EPO gene expression and a major contributor for HIF-1a induction in hepatocytes and Kupffer cells during acute-phase response. The increase of HIF-2a, predominantly expressed in endothelial cells and fibroblast-like cells, seems not to be affected by the lack of IL-6.

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“…Majority of plasmatic proteins is synthesized in hepatocytes, with albumin representing their largest quantitative part (Elmaouhoub et al 2007). Except for albumin (protein), all plasmatic proteins are glycoproteins.…”

mentioning

confidence: 99%

Levels of protein fractions in blood serum of periparturient goats

et al. 2011

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The aim of the study was to compare contents of individual protein fractions determined by electrophoresis in blood serum of healthy periparturient goats. Eight clinically healthy white shorthaired goats were examined. Blood samples of these goats were taken from v. jugularis three weeks and two weeks before the anticipated parturition, on the parturition day, 7 days after the parturition, and 28 days after the parturition. Individual protein fractions, albumins, α1-, α2-, β1-, β2-and γ-globulins were identified by electrophoretic analysis of blood serum. Percentage shares of individual protein fractions were converted from total protein values to g·l -1 values. The results indicated that individual serum protein fractions in periparturient goats showed significant dynamics of change. There were no significant differences between samples taken before the parturition and on the parturition day (except for the increase of β2 from 2.5 ± 0.3 g·l -1 observed 3 weeks before the parturition to 3.2 ± 0.4 g·l -1 measured 2 weeks before the parturition, and the decrease of γG levels from 9.5 ± 2.6 g·l -1 observed three weeks before the parturition to 8.1 ± 1.7 g·l -1 found on the parturition day), yet most indicators measured after the parturition were significantly higher (p < 0.05) than on the parturition day (values in g·l -1 observed on the parturition day versus values measured 28 days after the parturition: total protein 60.6 ± 4.3 vs. 71.3 ± 2.4, albumins 37.4 ± 2.9 vs. 42.7 ± 1.0, α2 3.4 ± 0.5 vs. 4.1 ± 0.8, β1 5.6 ± 0.5 vs. 6.3 ± 0.9, γG 8.1 ± 1.7 vs. 12.3 ± 1.9). The results bring new knowledge on the values of individual fractions of serum proteins in healthy goats, which is necessary for diagnostic interpretation of pathological findings in animals and also for experimental studies.

show abstract

Kinetics of albumin- and alpha-fetoprotein-production during rat liver development

Cited by 25 publications

References 33 publications

Smoothened is a master regulator of adult liver repair

Smoothened is a master regulator of adult liver repair

Cellular and molecular mechanisms regulating the hepatic erythropoietin expression during acute-phase response: a role for IL-6

Levels of protein fractions in blood serum of periparturient goats

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