Kinetics of acetophenone reduction to (<i>R</i>)-1-phenylethanol by a whole-cell<i>Pichia capsulata</i>biocatalyst

Homola, Patrik; Kurák, Tomáš; Illeová, Viera; Polakovič, Milan

doi:10.3109/10242422.2016.1151007

Cited by 8 publications

(3 citation statements)

References 22 publications

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“…Higher yield at 45 °C compared to 25 °C was due to H. volcanii growing optimally at 45 °C. This is in keeping with previous whole cell biocatalysis studies where organism’s temperature optimum yields the highest 1-phenylethanol product (Homola et al 2015 ; Illeová et al 2015 ; Kurbanoglu et al 2010 ). Lowest yield found at 50 rpm was probably due to slow agitation resulting in insufficient contact between substrate and biocatalyst.…”

Section: Discussionsupporting

confidence: 89%

Haloferax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems

Haque

Paradisi

Allers

2019

Appl Microbiol Biotechnol

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Enzyme-mediated synthesis of pharmaceutical compounds is a ‘green’ alternative to traditional synthetic chemistry, and microbial engineering opens up the possibility of using whole cells as mini-factories. Whole-cell biocatalysis reduces cost by eliminating expensive enzyme purification and cofactor addition steps, as well as resulting in increased enzyme stability. Haloferax volcanii is a model halophilic archaeon encoding highly salt and organic solvent tolerant enzymes such as alcohol dehydrogenase ( Hv ADH2), which catalyses the reduction of aldehydes and ketone in the presence of NADPH/NADH cofactor. A H. volcanii strain for constitutive Hv ADH2 expression was generated using a strong synthetic promoter (p. syn ). The strain was immobilised in calcium alginate beads and repeatedly used as a whole-cell biocatalyst. The reduction of acetophenone, used as test substrate, was very successful and high yields were detected from immobilised whole cells over repeated biotransformation cycles. The immobilised H. volcanii retained stability and high product yields after 1 month of storage at room temperature. This newly developed system offers halophilic enzyme expression in its native environment, high product yield, stability and reusability without the addition of any expensive NADPH/NADH cofactor. This is the first report of whole cell–mediated biocatalysis by the halophilic archaeon H. volcanii .

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Section: Discussionsupporting

confidence: 89%

Haloferax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems

Haque

Paradisi

Allers

2019

Appl Microbiol Biotechnol

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“…Acetophenone (ACP) is a kind of important chemical raw material and intermediate for the synthesis of some spices, dyes, and medicines. − It is often produced by the oxidation of ethylbenzene in industry. , Meanwhile, in the production of dicumyl peroxide from cumene, ACP can be produced in the byproduct including cumene, ACP, and other impurities, which is mainly used as a mixture and thus not economically efficient. There are few reports about the separation of ACP and cumene.…”

Section: Introductionmentioning

confidence: 99%

Isobaric Vapor–Liquid Equilibrium for the Binary System of Cumene + Acetophenone at 101.3, 30.0, and 10.0 kPa

Zhai

Tian

et al. 2022

J. Chem. Eng. Data

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The isobaric binary vapor–liquid equilibrium (VLE) data for the mixture of cumene and acetophenone were studied at 101.3, 30.0, and 10.0 kPa in a modified Rose equilibrium still. This system exhibited no azeotropic behavior at the studied pressures. The consistency of the VLE data was verified by both the Fredenslund test and Redlich–Kister area test. Meanwhile, the experimental data were correlated by the nonrandom two-liquid, universal quasichemical, and Wilson models. The corresponding binary interaction parameters for the three models, which could contribute to the simulation and design for the separation process, were obtained using a maximum likelihood objective function. All three models showed good agreement with the experimental data.

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“…In many cases, the elimination of environmentally harmful chemical processes can be achieved using a biocatalyst. For this purpose, enzymes are often used in the form of various preparations such as concentrated solutions, powders, or biomass (Choi et al, 2019;Di Cosimo et al, 2013;Homola et al, 2015;Tozlu et al, 2019). However, the protein structure of enzymes makes them very sensitive to external factors, and they require strictly defined and controlled process conditions.…”

Section: Introductionmentioning

confidence: 99%

Use of the chemical permeabilization process in yeast cells: production of high-activity whole cell biocatalysts

Trawczyńska¹

2020

bta

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Yeast cells are popular microorganisms for use in various bioprocesses because of their ability to produce various enzymes. They are also known for their low price. However, the activity of these biocatalysts is limited by cell membrane diffusion. One of the possible solutions to this problem is permeabilization, which makes cell walls and membranes of microorganisms more permeable to allow for easier reagent diffusion, while also maintaining properties of the cells, such as their structure and enzymatic activity. The reusability of permeabilized baker's yeast cells is a major asset that allows them to be successfully used in biotransformation processes. Because of extensive research on this process, numerous procedures have been developed regarding the production of highly active biocatalysts in the form of permeabilized cells. The most commonly used technique is chemical permeabilization. Its effectiveness is based on the activity of various intracellular enzymes: one enzyme or several enzymes simultaneously. Multiple chemical substances, mostly organic solvents and detergents, were analyzed to determine their effectiveness as permeabilizing agents. This review provides a state-of-the-science analysis of substances used as permeabilizing agents for yeast permeabilization.

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Kinetics of acetophenone reduction to (R)-1-phenylethanol by a whole-cellPichia capsulatabiocatalyst

Cited by 8 publications

References 22 publications

Haloferax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems

Haloferax volcanii as immobilised whole cell biocatalyst: new applications for halophilic systems

Isobaric Vapor–Liquid Equilibrium for the Binary System of Cumene + Acetophenone at 101.3, 30.0, and 10.0 kPa

Use of the chemical permeabilization process in yeast cells: production of high-activity whole cell biocatalysts

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