Kinetics for the subgel phase formation in DPPC/DOPC mixed bilayers

Kinoshita, Masanao; Ito, Keisuke; Kato, Satoru

doi:10.1016/j.chemphyslip.2010.06.007

Cited by 6 publications

(4 citation statements)

References 42 publications

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“…The formation of these lamellae is corroborated with FFEM micrographs at 25 C. This subtransition could also be due to the fact that, for the formation of the DPPC/DHPC/FFA systems, more thermal treatments were performed than in the case of the DPPC/DHPC bicelles. This is in accordance with the results provided by Kinoshita et al 64 that reported that an incubation at low temperatures induces the formation of a subgel transition, namely, a subtransition, in our case. The pretransition double shoulder and the detected subtransition reinforce the hypothesis of the formation of new complexes of DPPC, DHPC and FFA or different combinations of the three.…”

Section: Discussionsupporting

confidence: 94%

Structural effects of flufenamic acid in DPPC/DHPC bicellar systems

et al. 2011

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The effect of the anti-inflammatory flufenamic acid (FFA) in dipalmitoylphosphatidylcholine (DPPC)/ dihexanoylphosphatidylcholine (DHPC) bicellar systems has been investigated. For this purpose, a combination of different techniques was used, including dynamic light scattering (DLS), freezefracture electron microscopy (FFEM), cryo-transmission electron microscopy (Cryo-TEM), X-ray scattering and differential scanning calorimetry (DSC). The incorporation of FFA induced changes in the size, morphology, thermotropic behaviour and self-assembly of the initial bicellar systems. At the three temperatures studied, different structures were formed as a consequence of the incorporation of the drug in the bicelles. At 25 C, the formation of large multilamellar sheets was observed, and at 32.5 C, the vesicle size decreased, and undulated structures and discoidal bicelles appeared. At 37 C, tubular, discoidal and spherical small structures were detected. Using this experimental approach, we have attempted to characterise these bicellar systems containing FFA, which may be very promising as new vehicles for the topical application of active principles.

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Section: Discussionsupporting

confidence: 94%

Structural effects of flufenamic acid in DPPC/DHPC bicellar systems

et al. 2011

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“…The T m gave rise to an intense, sharp peak at B42 1C, 13 the T p is a less intense broad peak at 31-33 1C, 13 whilst the T s is not observed in the thermogram, which is common for lipid bilayers. 28 This confirms that the phase transitions specific to DPPC are retained upon addition of the CPE and correlate well with the observed spectral changes. In addition, the negligible effect on the shape or position of the T m profile at B41 1C upon addition of P3Anionic signifies the absence of any substantial lipid reorganisation due to CPE binding.…”

supporting

confidence: 81%

“…Moreover, to the best of our knowledge, there is no COE/CPE probe reported to date that is able to detect the less energetically demanding liquid crystal to lamellar gel transformation, known as the sub-transition, T s . 28 Here, we report for the first time a red-emitting, anionic poly(thiophene) CPE, poly [3-(6-sulfothioatehexyl)thiophene], that can be used to sequentially monitor the three primary phase transitions in model lipid cell membranes using a single probe. P3Anionic (M n = 4990 g mol À1 , PDI = 1.13) was synthesised as previously reported (Fig.…”

mentioning

confidence: 99%

Sequential detection of multiple phase transitions in model biological membranes using a red-emitting conjugated polyelectrolyte

Houston

Kraft

Scherf

et al. 2016

Phys. Chem. Chem. Phys.

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The anionic conjugated polyelectrolyte, poly[3-(6-sulfothioatehexyl)thiophene] (P3Anionic), functions as a highly sensitive probe of membrane order, uniquely capable of sequentially detecting the three key phase transitions occurring within model phospholipid bilayers. The observed sensitivity is the result of charge-mediated, selective localisation of P3Anionic within the head-groups of the phospholipid bilayer.

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“…Since the pre-transition is a result of changes in acyl chain packing, its perturbation and/or vanishing indicates that TTA probably influences the structure of the lipid bilayer. On the other hand, compounds that incorporate into the polar-nonpolar region of the lipid bilayer abolish the DPPC pre-transition even at low concentrations [31][32][33]. Thus, we concluded that both TTA compounds interact with membrane phospholipids and may be localized rather on the surface of lipid bilayers and interact with the DPPC head group.…”

Section: Resultsmentioning

confidence: 86%

Lipid and DNA interaction with the triorganotin dimethylaminophenylazobenzoates studied by DSC and spectroscopy methods

Pruchnik

Kral

Hof

2018

J Therm Anal Calorim

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The aim of the study was to investigate and compare the interaction of tributyltin (TBTA) and triphenyltin (TPhTA) 2-[4 (dimethylamino)phenylazo]benzoates complexes with the lipid membrane and plasmid DNA. Both TBTA and TPhTA belong to the group of promising anticancer agents. Differential scanning calorimetry method was employed to examine the heat signal associated with phase transitions of liposomes formed from dipalmitoylphosphatidylcholine (DPPC) or DPPC/cholesterol and modified with TBTA and TPhTA. The investigated compounds influence the thermotropic parameters of the DPPC and DPPC/cholesterol lipid bilayer in a slightly different way: Although the temperature of the main phase transition of the DPPC remains almost unaffected, the addition of TPhTA can abolish sub-and pre-transition when added in sufficiently high concentrations. At the same time, the addition of the TBTA practically does not change the sub-transition and only slightly influences the pre-transition. The effect of organotin compounds on plasmid DNA was investigated using single-molecule florescence technique and time-correlated single-photon counting fluorescence correlation spectroscopy. Influence of TBTA and TPhTA on DNA resulted only in fractional conformation change which did not lead to the tightly packed conformation however, analysis of the diffusion times indicates that TPhTA interacts with DNA in a different mode than TBTA.

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Kinetics for the subgel phase formation in DPPC/DOPC mixed bilayers

Cited by 6 publications

References 42 publications

Structural effects of flufenamic acid in DPPC/DHPC bicellar systems

Structural effects of flufenamic acid in DPPC/DHPC bicellar systems

Sequential detection of multiple phase transitions in model biological membranes using a red-emitting conjugated polyelectrolyte

Lipid and DNA interaction with the triorganotin dimethylaminophenylazobenzoates studied by DSC and spectroscopy methods

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