Kinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor

Nielsen, Marie Vejrup; Bollmann, Annette; Sliekers, Olav; Jetten, Mike S. M.; Schmid, Markus; Strous, Marc; Schmidt, Ingo; Larsen, Lars; Nielsen, Lars Peter; Revsbech, Niels Peter

doi:10.1016/j.femsec.2004.09.003

Cited by 183 publications

(90 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Assuming that these aerobic processes also occur in association with the particles, it is proposed that aerobic metabolisms occurring at the periphery of the oxygenated particle fuel the internal anaerobic pathways by the diffusion of their products (e.g. nitrite produced by AO) to the respiration-mediated anoxic core, similarly to the scenarios described for various other microbial aggregates in wastewater treatment plants (Nielsen et al, 2005), man-made model systems (Stief et al, 2016) and natural environments (Klawonn et al, 2015). On the other hand, the detection of nitrification during anoxic incubations has been attributed to the persistence of traces of O 2 in the samples (e.g.…”

Section: Environmental Variabilitymentioning

confidence: 99%

Vertical segregation among pathways mediating nitrogen loss (N2 and N2O production) across the oxygen gradient in a coastal upwelling ecosystem

2017

View full text Add to dashboard Cite

Abstract. The upwelling system off central Chile (36.5 • S) is seasonally subjected to oxygen (O 2 )-deficient waters, with a strong vertical gradient in O 2 (from oxic to anoxic conditions) that spans a few metres (30-50 m interval) over the shelf. This condition inhibits and/or stimulates processes involved in nitrogen (N) removal (e.g. anammox, denitrification, and nitrification). During austral spring (September 2013) and summer (January 2014), the main pathways involved in N loss and its speciation, in the form of N 2 and/or N 2 O, were studied using 15 N-tracer incubations, inhibitor assays, and the natural abundance of nitrate isotopes along with hydrographic information. Incubations were developed using water retrieved from the oxycline (25 m depth) and bottom waters (85 m depth) over the continental shelf off Concepción, Chile. Results of 15 N-labelled incubations revealed higher N removal activity during the austral summer, with denitrification as the dominant N 2 -producing pathway, which occurred together with anammox at all times. Interestingly, in both spring and summer maximum potential N removal rates were observed in the oxycline, where a greater availability of oxygen was observed (maximum O 2 fluctuation between 270 and 40 µmol L −1 ) relative to the hypoxic bottom waters (< 20 µmol O 2 L −1 ). Different pathways were responsible for N 2 O produced in the oxycline and bottom waters, with ammonium oxidation and dissimilatory nitrite reduction, respectively, as the main source processes. Ammonium produced by dissimilatory nitrite reduction to ammonium (DNiRA) could sustain both anammox and nitrification rates, including the ammonium utilized for N 2 O production. The temporal and vertical variability of δ 15 N-NO − 3 confirms that multiple N-cycling processes are modulating the isotopic nitrate composition over the shelf off central Chile during spring and summer. N removal processes in this coastal system appear to be related to the availability and distribution of oxygen and particles, which are a source of organic matter and the fuel for the production of other electron donors (i.e. ammonium) and acceptors (i.e. nitrate and nitrite) after its remineralization. These results highlight the links between several pathways involved in N loss. They also establish that different mechanisms supported by alternative N substratesPublished by Copernicus Publications on behalf of the European Geosciences Union. 4796A. Galán et al.: Vertical segregation among pathways mediating nitrogen loss are responsible for substantial accumulation of N 2 O, which are frequently observed as hotspots in the oxycline and bottom waters. Considering the extreme variation in oxygen observed in several coastal upwelling systems, these findings could help to understand the ecological and biogeochemical implications due to global warming where intensification and/or expansion of the oceanic OMZs is projected.

show abstract

Section: Environmental Variabilitymentioning

confidence: 99%

Vertical segregation among pathways mediating nitrogen loss (N2 and N2O production) across the oxygen gradient in a coastal upwelling ecosystem

2017

View full text Add to dashboard Cite

show abstract

“…Thickness of the biofilm or size of the granular sludge influences bacteria composition as well. Large aggregates (> 500 μm) account for 68% of the ANAMMOX bacteria, while small aggregates (<500 μm) only account for 35% (Nielsen et al, 2005). The reason is obvious.…”

Section: Completely Autotrophic Nitrogen Removal Over Nitrite (Canon)mentioning

confidence: 99%

“…Subsequently, the produced nitrite, an inhibitor to AOB, is used as electron acceptor by the ANAMMOX bacteria. Thus, a symbiosis between AOB and the ANAMMOX bacteria is finely established as indicated by fluorescence in situ hybridization (Sliekers et al, 2002;Nielsen et al, 2005). For ammonium-limited or DO-excess influent, ammonium is exhausted by the AOB rapidly.…”

Section: Completely Autotrophic Nitrogen Removal Over Nitrite (Canon)mentioning

confidence: 99%

“…Thus, the stoichiometry of ammonium and oxygen is the key control parameter in the CANON process. For fluctuating ammonium loading rates in engineering, DO can be regulated through on-line feed-back control (Nielsen et al, 2005).…”

Section: Completely Autotrophic Nitrogen Removal Over Nitrite (Canon)mentioning

confidence: 99%

“…For wastewaters with higher ammonium, it is worth sacrificing relatively high investment for a partial nitritation-ANAMMOX practice. The expenses will be compensated by lower operational costs and efficient nitrogen removal performance (Hao et al, 2001;Nielsen et al, 2005).…”

Section: Completely Autotrophic Nitrogen Removal Over Nitrite (Canon)mentioning

confidence: 99%

See 2 more Smart Citations

Anaerobic ammonium oxidation for treatment of ammonium-rich wastewaters

Zhang

Zheng

Tang

et al. 2008

J. Zhejiang Univ. Sci. B

View full text Add to dashboard Cite

Abstract:The concept of anaerobic ammonium oxidation (ANAMMOX) is presently of great interest. The functional bacteria belonging to the Planctomycete phylum and their metabolism are investigated by microbiologists. Meanwhile, the ANAMMOX is equally valuable in treatment of ammonium-rich wastewaters. Related processes including partial nitritation-ANAMMOX and completely autotrophic nitrogen removal over nitrite (CANON) have been developed, and lab-scale experiments proved that both processes were quite feasible in engineering with appropriate control. Successful full-scale practice in the Netherlands will accelerate application of the process in future. This review introduces the microbiology and more focuses on application of the ANAMMOX process.

show abstract

The Metabolism of Anammox

Kartal

Keltjens

Jetten

2008

Encyclopedia of Life Sciences

View full text Add to dashboard Cite

Anaerobic ammonium‐oxidizing (anammox) bacteria derive their energy for growth from the conversion of ammonium and nitrite into dinitrogen gas. The bacteria are the latest addition to the biogeochemical nitrogen cycle. The slowly growing micro‐organisms that belong to the Planctomycetes are characterized by a compartmentalized cell architecture featuring a central cell organelle, the ‘anammoxosome’. Membrane systems surrounding the different cellular compartments are composed of unique ‘ladderane’ lipid molecules. Although the metabolism has not been completely resolved, nitrogen formation seems to involve the intermediary formation of hydrazine, a most reactive and toxic compound. Anammox bacteria have been detected in many oxygen‐limited freshwater and marine ecosystems investigated. In the marine environment, over 50% of the nitrogen gas released is made by anammox bacteria. Application of the anammox process offers an attractive alternative to current wastewater treatment systems for the removal of fixed nitrogen. Key concepts Ammonium is converted under anaerobic conditions into dinitrogen gas by a newly discovered and specialized group of micro‐organisms, the anammox bacteria. Anammox bacteria are chemolithoautotrophic organisms that gain their energy for growth from the conversion of the inorganic substrates ammonium and nitrite. Cell carbon is derived from carbon dioxide. Anammox bacteria are characterised by a unique cell plan featuring a central cell organelle, the anammoxosome. The lipids from anammox bacteria are composed of highly unusual ‘ladderane’ molecules. The conversion of ammonium and nitrite into dinitrogen gas proceeds through the intermediary formation of the toxic and highly reactive hydrazine. Anammox bacteria synthesize adenosine triphosphate (ATP) by an electrochemical proton gradient most likely established across the anammoxosome membrane. In nature approximately 50% of the dinitrogen gas released into the atmosphere is made by anammox bacteria. Anammox bacteria offer an economically attractive and environmentally friendly alternative to current wastewater treatment processes for the removal of fixed nitrogen.

show abstract

Kinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor

Cited by 183 publications

References 41 publications

Vertical segregation among pathways mediating nitrogen loss (N<sub>2</sub> and N<sub>2</sub>O production) across the oxygen gradient in a coastal upwelling ecosystem

Vertical segregation among pathways mediating nitrogen loss (N<sub>2</sub> and N<sub>2</sub>O production) across the oxygen gradient in a coastal upwelling ecosystem

Anaerobic ammonium oxidation for treatment of ammonium-rich wastewaters

The Metabolism of Anammox

Contact Info

Product

Resources

About

Kinetics, diffusional limitation and microscale distribution of chemistry and organisms in a CANON reactor

Cited by 183 publications

References 41 publications

Vertical segregation among pathways mediating nitrogen loss (N&lt;sub&gt;2&lt;/sub&gt; and N&lt;sub&gt;2&lt;/sub&gt;O production) across the oxygen gradient in a coastal upwelling ecosystem

Vertical segregation among pathways mediating nitrogen loss (N&lt;sub&gt;2&lt;/sub&gt; and N&lt;sub&gt;2&lt;/sub&gt;O production) across the oxygen gradient in a coastal upwelling ecosystem

Anaerobic ammonium oxidation for treatment of ammonium-rich wastewaters

The Metabolism of Anammox

Contact Info

Product

Resources

About

Vertical segregation among pathways mediating nitrogen loss (N<sub>2</sub> and N<sub>2</sub>O production) across the oxygen gradient in a coastal upwelling ecosystem

Vertical segregation among pathways mediating nitrogen loss (N<sub>2</sub> and N<sub>2</sub>O production) across the oxygen gradient in a coastal upwelling ecosystem