Kinetics, cross-reactivity, and specificity of Bet v 1-specific IgG4 antibodies induced by immunotherapy with birch pollen

Subbarayal, Brinda; Schiller, Dirk; Möbs, Christian; Jong, Nicolette W. de; Ebner, Christof; Reider, Norbert; Bartel, Detlef; Lidholm, Jonas; Pfützner, Wolfgang; Wijk, Roy Gerth van; Vieths, Stefan; Bohle, Barbara

doi:10.1111/all.12236

Cited by 49 publications

(59 citation statements)

References 37 publications

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“…This pathophysiology is reminiscent of other, so‐called secondary food allergies, for example birch pollen‐related food allergy. Here, sensitization occurs to Bet v 1 via the respiratory tract and allergic reactions to foods are caused by IgE reactivity to similar conformational epitopes on structurally related food proteins, for example Mal d 1 in apple 29. We present 20 Austrian patients with delayed meat allergy and IgE reactivity to α‐gal on bovine IgG.…”

Section: Discussionmentioning

confidence: 99%

The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy

Kollmann

Nagl

Ebner³

et al. 2016

Allergy

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BackgroundIgG to galactose‐α‐1,3‐galactose (α‐gal) are highly abundant natural antibodies (Ab) in humans. α‐Gal‐specific IgE Ab cause a special form of meat allergy characterized by severe systemic reactions 3–7 h after consumption of red meat. We investigated 20 patients who experienced such reactions and characterized their α‐gal‐specific IgE and IgG responses in more detail.Methodsα‐Gal‐specific IgE was determined by ImmunoCAP. IgE reactivity to meat extract and bovine gamma globulin (BGG) was assessed by immunoblotting and ELISA, respectively. In some experiments, sera were pre‐incubated with α‐gal or protein G to deplete IgG Ab. α‐Gal‐specific IgG1–4 Ab in individuals with and without meat allergy were assessed by ELISA.ResultsIn immunoblots, BGG was the most frequently recognized meat protein. Binding of IgE and IgG to BGG was confirmed by ELISA and completely abolished after pre‐incubation with α‐gal. Neither the depletion of autologous α‐gal‐specific IgG Ab nor the addition of α‐gal‐specific IgG Ab from nonallergic individuals changed the IgE recognition of BGG of meat‐allergic patients. Meat‐allergic patients showed significantly higher α‐gal‐specific IgG1 and IgG3 Ab than nonallergic individuals, whereas the latter showed significantly higher levels of α‐gal‐specific IgG4 Ab.ConclusionPatients with delayed meat allergy display IgE and IgG Ab that selectively recognize the α‐gal epitope on BGG. Their enhanced α‐gal‐specific IgE levels are accompanied by high levels of α‐gal‐specific IgG1 devoid of IgE‐blocking activity. This subclass distribution is atypical for food allergies and distinct from natural α‐gal IgG responses in nonallergic individuals.

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Section: Discussionmentioning

confidence: 99%

The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy

Kollmann

Nagl

Ebner³

et al. 2016

Allergy

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“…1 In a different type of AIT, namely, subcutaneous treatment with birch pollen extract (BP-AIT) containing 5% to 10% of the sole major allergen Bet v 1, we have reported that most Bet v 1-specific IgG 4 antibodies induced after 36 months recognized IgE epitopes and only a minority represented de novo specificities. 2 In the present longitudinal study, we sought to investigate whether the diversity of Bet v 1-specific IgG 4 antibodies expands, decreases, or remains similar in the course of BP-AIT.…”

Section: To the Editormentioning

confidence: 99%

“…2 Briefly, each serum sample was preincubated with Bet v 1 and the same indicator serum containing more than 100 kU/L of Bet v 1-specific IgE with a presumed highly diverse IgE repertoire. Thereafter, the activity of each serum to reduce the binding of Bet v 1-IgE complexes to CD23 1 B cells was determined.…”

Section: To the Editormentioning

confidence: 99%

The diversity of Bet v 1–specific IgG 4 antibodies remains mostly constant during the course of birch pollen immunotherapy

Subbarayal

Schiller

Möbs

et al. 2015

Journal of Allergy and Clinical Immunology

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“…Although the BT-specific IgG 4 increase was small, it was statistically significant. This may explain part of the therapeutic effect, similar to the cross-reactive IgG 4 to tree species, such as alder and hazel, induced with birch pollen immunotherapy [35]. …”

Section: Discussionmentioning

confidence: 99%

Clinical Response to Subcutaneous Dermatophagoides pteronyssinus Immunotherapy in Children with Allergic Rhinitis and Asthma Is Independent of Sensitization to Blomia tropicalis Allergens

Shi

Zheng²,

Chen

et al. 2018

Int Arch Allergy Immunol

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Background: Dermatophagoides pteronyssinus (DP) and Blomia tropicalis (BT) are the dominant house dust mites inducing allergic diseases in tropical climates. It is not known whether the efficacy of DP subcutaneous immunotherapy (SCIT) is similar in patients sensitized to DP alone or to both DP and BT. Method: Ninety-five children (5–17 years old) affected by asthma with rhinitis and sensitized to both DP and BT received 3 years of DP-SCIT. Clinical symptom and medication scores, serum-specific IgE and IgG₄ were evaluated during DP-SCIT. Patients were grouped based on DP and BT co-sensitization or cross-reactivity, according to positive or negative IgE to BT major allergen (BTMA). Results: After 3 years of DP-SCIT, all patients had significant reductions in symptoms and medication use. In all, 65% of the patients were free of asthma symptoms and medication use; in addition, 3% was free of rhinitis symptoms. FEV₁ in all patients were greater than 95% of predicted. DP-SCIT induced significant increases in DP- and BT-specific IgG₄. In 50% of patients, DP-specific IgG₄ increased more than 67-fold. BT-specific IgG₄ increased more than 2.5 fold. A moderate correlation (r = 0.48–0.61, p < 0.01) was found between specific IgE against DP and BT in the BTMA– group (n = 34) before and after DP-SCIT, whereas no correlation was found in the BTMA+ group (n = 61). The 2 BTMA groups responded similarly with regard to clinical improvement and increase in specific IgG₄ to both DP and BT. No safety finding of concern were reported in either group. Conclusion: DP-SCIT may be of clinical benefit to patients with IgE sensitizations to both DP and BT. DP-SCIT induces IgG₄ that cross-react with BT allergens.

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Kinetics, cross-reactivity, and specificity of Bet v 1-specific IgG4 antibodies induced by immunotherapy with birch pollen

Abstract: Our results indicate that BP-SIT may induce Bet v 1-specific IgG4 antibodies that cross-react with related food allergens and inhibit IgE binding by epitope competition.

Cited by 49 publications

References 37 publications

The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy

The quantity and quality of α-gal-specific antibodies differ in individuals with and without delayed red meat allergy

The diversity of Bet v 1–specific IgG 4 antibodies remains mostly constant during the course of birch pollen immunotherapy

Clinical Response to Subcutaneous <b><i>Dermatophagoides pteronyssinus</i></b> Immunotherapy in Children with Allergic Rhinitis and Asthma Is Independent of Sensitization to <b><i>Blomia tropicalis</i></b> Allergens

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